ATG3 Antibody (PACO25108)
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주문- SKU:
- PACO25108
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Antibody Name: | ATG3 Antibody (PACO25108) |
Antibody SKU: | PACO25108 |
Size: | 50ug |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB, IHC, IF |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:1000-1:2000, IF:1:200-1:500 |
Species Reactivity: | Human |
Immunogen: | Recombinant Human Ubiquitin-like-conjugating enzyme ATG3 protein (1-314AA) |
Form: | Liquid |
Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
Purification Method: | >95%, Protein G purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
Western Blot Positive WB detected in: K562 whole cell lysate, Hela whole cell lysate, Jurkat whole cell lysate, PC-3 whole cell lysate All lanes: ATG3 antibody at 4µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 36 kDa Observed band size: 36 kDa . | |
IHC image of PACO25108 diluted at 1:1200 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. | |
IHC image of PACO25108 diluted at 1:1200 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
Background: | E2 conjugating enzyme required for the cytoplasm to vacuole transport (Cvt), autophagy, and mitochondrial homeostasis. Responsible for the E2-like covalent binding of phosphatidylethanolamine to the C-terminal Gly of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A). The ATG12-ATG5 conjugate plays a role of an E3 and promotes the transfer of ATG8-like proteins from ATG3 to phosphatidylethanolamine (PE). This step is required for the membrane association of ATG8-like proteins. The formation of the ATG8-phosphatidylethanolamine conjugates is essential for autophagy and for the cytoplasm to vacuole transport (Cvt). Preferred substrate is MAP1LC3A. Also acts as an autocatalytic E2-like enzyme, catalyzing the conjugation of ATG12 to itself, ATG12 conjugation to ATG3 playing a role in mitochondrial homeostasis but not in autophagy. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway. |
Synonyms: | Ubiquitin-like-conjugating enzyme ATG3 (EC 2.3.2.-) (Autophagy-related protein 3) (APG3-like) (hApg3) (Protein PC3-96), ATG3, APG3 APG3L |
UniProt Protein Function: | ATG3: an E2-like enzyme essential for Apg8p lipidation. |
UniProt Protein Details: | Protein type:Ubiquitin conjugating system; Enzyme, cellular metabolism; Autophagy; EC 6.3.2.- Chromosomal Location of Human Ortholog: 3q13.2 Cellular Component: cytoplasmic ubiquitin ligase complex; cytosol Molecular Function:APG12 conjugating enzyme activity; APG8 conjugating enzyme activity; enzyme binding; protein binding; small conjugating protein ligase activity Biological Process: autophagic vacuole formation; macroautophagy; mitochondrial fragmentation during apoptosis; mitochondrion degradation; protein modification process; protein targeting to membrane; protein ubiquitination |
NCBI Summary: | This gene encodes a ubiquitin-like-conjugating enzyme and is a component of ubiquitination-like systems involved in autophagy, the process of degradation, turnover and recycling of cytoplasmic constituents in eukaryotic cells. This protein is known to play a role in regulation of autophagy during cell death. A pseudogene of this gene is located on chromosome 20. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2013] |
UniProt Code: | Q9NT62 |
NCBI GenInfo Identifier: | 61212142 |
NCBI Gene ID: | 64422 |
NCBI Accession: | Q9NT62.1 |
UniProt Secondary Accession: | Q9NT62,Q6PKC5, Q9H6L9, |
UniProt Related Accession: | Q9NT62 |
Molecular Weight: | 35,467 Da |
NCBI Full Name: | Ubiquitin-like-conjugating enzyme ATG3 |
NCBI Synonym Full Names: | autophagy related 3 |
NCBI Official Symbol: | ATG3 |
NCBI Official Synonym Symbols: | APG3; APG3L; PC3-96; APG3-LIKE |
NCBI Protein Information: | ubiquitin-like-conjugating enzyme ATG3 |
UniProt Protein Name: | Ubiquitin-like-conjugating enzyme ATG3 |
UniProt Synonym Protein Names: | Autophagy-related protein 3; APG3-like; hApg3; Protein PC3-96 |
Protein Family: | Autophagy-related protein |
UniProt Gene Name: | ATG3 |
UniProt Entry Name: | ATG3_HUMAN |