AS3MT Antibody (PACO57564)
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주문- SKU:
- PACO57564
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Antibody Name: | AS3MT Antibody (PACO57564) |
Antibody SKU: | PACO57564 |
Size: | 50ug |
Host Species: | Rabbit |
Tested Applications: | ELISA, IHC, IF |
Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:200-1:500, IF:1:50-1:200 |
Species Reactivity: | Human |
Immunogen: | Recombinant Human Arsenite methyltransferase protein (262-375AA) |
Form: | Liquid |
Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Purification Method: | >95%, Protein G purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
IHC image of PACO57564 diluted at 1:400 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. | |
Immunofluorescence staining of HepG2 cells with PACO57564 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
Background: | Catalyzes the transfer of a methyl group from AdoMet to trivalent arsenicals producing methylated and dimethylated arsenicals. It methylates arsenite to form methylarsonate, Me-AsO(3)H(2), which is reduced by methylarsonate reductase to methylarsonite, Me-As(OH)2. Methylarsonite is also a substrate and it is converted into the much less toxic compound dimethylarsinate (cacodylate), Me(2)As(O)-OH (By similarity). |
Synonyms: | Arsenite methyltransferase (EC 2.1.1.137) (Methylarsonite methyltransferase) (S-adenosyl-L-methionine:arsenic(III) methyltransferase), AS3MT, CYT19 |
UniProt Protein Function: | AS3MT: Catalyzes the transfer of a methyl group from AdoMet to trivalent arsenicals producing methylated and dimethylated arsenicals. It methylates arsenite to form methylarsonate, Me- AsO(3)H(2), which is reduced by methylarsonate reductase to methylarsonite, Me-As(OH)2. Methylarsonite is also a substrate and it is converted into the much less toxic compound dimethylarsinate (cacodylate), Me(2)As(O)-OH. Belongs to the methyltransferase superfamily. |
UniProt Protein Details: | Protein type:EC 2.1.1.137; Methyltransferase; Mitochondrial Chromosomal Location of Human Ortholog: 10q24.32 Cellular Component: mitochondrion; cytoplasm; cytosol Molecular Function:arsenite methyltransferase activity; methylarsonite methyltransferase activity; S-adenosylmethionine-dependent methyltransferase activity Biological Process: methylation; arsonoacetate metabolic process; toxin metabolic process |
NCBI Summary: | AS3MT catalyzes the transfer of a methyl group from S-adenosyl-L-methionine (AdoMet) to trivalent arsenical and may play a role in arsenic metabolism (Lin et al., 2002 [PubMed 11790780]).[supplied by OMIM, Mar 2008] |
UniProt Code: | Q9HBK9 |
NCBI GenInfo Identifier: | 109389364 |
NCBI Gene ID: | 57412 |
NCBI Accession: | NP_065733.2 |
UniProt Secondary Accession: | Q9HBK9,Q0VDK3, Q0VDK4, Q5PZ02, A6NP79, |
UniProt Related Accession: | Q9HBK9 |
Molecular Weight: | 31,098 Da |
NCBI Full Name: | arsenite methyltransferase |
NCBI Synonym Full Names: | arsenite methyltransferase |
NCBI Official Symbol: | AS3MT |
NCBI Official Synonym Symbols: | CYT19 |
NCBI Protein Information: | arsenite methyltransferase; methyltransferase cyt19; methylarsonite methyltransferase; arsenic (+3 oxidation state) methyltransferase; S-adenosylmethionine:arsenic (III) methyltransferase; S-adenosyl-L-methionine:arsenic(III) methyltransferase |
UniProt Protein Name: | Arsenite methyltransferase |
UniProt Synonym Protein Names: | Methylarsonite methyltransferase; S-adenosyl-L-methionine:arsenic(III) methyltransferase |
Protein Family: | Arsenite methyltransferase |
UniProt Gene Name: | AS3MT |
UniProt Entry Name: | AS3MT_HUMAN |
Antibodies |
Anti-AS3MT Antibody (CAB16138) |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
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