ARHGEF7 Antibody (PACO15517)
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주문- SKU:
- PACO15517
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Description
Antibody Name: | ARHGEF7 Antibody (PACO15517) |
Antibody SKU: | PACO15517 |
Size: | 50ul |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB, IHC |
Recommended Dilutions: | ELISA:1:2000-1:5000, WB:1:500-1:2000, IHC:1:50-1:200 |
Species Reactivity: | Human, Mouse, Rat |
Immunogen: | Fusion protein of human ARHGEF7 |
Form: | Liquid |
Storage Buffer: | -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol |
Purification Method: | Antigen affinity purification |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using PACO15517(ARHGEF7 Antibody) at dilution 1/40, on the right is treated with fusion protein. (Original magnification: x200). | |
Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane 1-3: Human brain malignant glioma tissue, A172 cells, 293T cells, Primary antibody: PACO15517(ARHGEF7 Antibody) at dilution 1/800, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 3 minutes. | |
The image on the left is immunohistochemistry of paraffin-embedded Human gastic cancer tissue using PACO15517(ARHGEF7 Antibody) at dilution 1/40, on the right is treated with fusion protein. (Original magnification: x200). |
Background: | Rho GTPases play a fundamental role in numerous cellular processes triggered by extracellular stimuli that work through G protein coupled receptors. The encoded protein belongs to a family of cytoplasmic proteins that activate the Ras-like family of Rho proteins by exchanging bound GDP for GTP. It forms a complex with the small GTP binding protein Rac1 and recruits Rac1 to membrane ruffles and to focal adhesions. This protein can induce membrane ruffling. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene. |
Synonyms: | Rho guanine nucleotide exchange factor (GEF) 7 |
UniProt Protein Function: | ARHGEF7: a RAC1 guanine nucleotide exchange factor (GEF). Can induce membrane ruffling. Interacts with PAK kinases through its SH3 domain. Acts both as an activator and a downstream target for Cdc42. Its EGF-dependent phosphorylation activates its nucleotide exchange factor for Cdc42 and regulates its interaction with the E3 ligase Cbl, thus modulating Cbl-catalysed EGFR degradation. Its phosphorylation status is necessary to maintain the balance between normal signaling by EGFR and Src versus aberrant growth and transformation. Four alternatively spliced isoforms have been described. |
UniProt Protein Details: | Protein type:GEFs; GEFs, Rac/Rho; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 13q34 Cellular Component: cell cortex; cell soma; cytosol; focal adhesion; lamellipodium; neuron projection; plasma membrane; protein complex; ruffle Molecular Function:guanyl-nucleotide exchange factor activity; protein binding; protein kinase binding; Rho guanyl-nucleotide exchange factor activity Biological Process: ephrin receptor signaling pathway; focal adhesion formation; Golgi organization and biogenesis; hemopoietic progenitor cell differentiation; lamellipodium biogenesis; negative regulation of epidermal growth factor receptor signaling pathway; nervous system development; positive regulation of apoptosis; positive regulation of growth hormone secretion; positive regulation of GTPase activity; positive regulation of protein binding; regulation of Rho protein signal transduction; regulation of small GTPase mediated signal transduction; signal transduction |
NCBI Summary: | This gene encodes a protein that belongs to a family of cytoplasmic proteins that activate the Ras-like family of Rho proteins by exchanging bound GDP for GTP. It forms a complex with the small GTP binding protein Rac1 and recruits Rac1 to membrane ruffles and to focal adhesions. Multiple alternatively spliced transcript variants encoding different isoforms have been observed for this gene. [provided by RefSeq, Mar 2016] |
UniProt Code: | Q14155 |
NCBI GenInfo Identifier: | 50403776 |
NCBI Gene ID: | 8874 |
NCBI Accession: | Q14155.2 |
UniProt Secondary Accession: | Q14155,Q3LIA4, Q5W9H0, Q6P9G3, Q6PII2, Q86W63, Q8N3M1 B1ALK6, B1ALK8, |
UniProt Related Accession: | Q14155 |
Molecular Weight: | 70,450 Da |
NCBI Full Name: | Rho guanine nucleotide exchange factor 7 |
NCBI Synonym Full Names: | Rho guanine nucleotide exchange factor 7 |
NCBI Official Symbol: | ARHGEF7 |
NCBI Official Synonym Symbols: | P50; P85; PAK3; PIXB; COOL1; P50BP; COOL-1; P85SPR; BETA-PIX; P85COOL1; Nbla10314 |
NCBI Protein Information: | rho guanine nucleotide exchange factor 7 |
UniProt Protein Name: | Rho guanine nucleotide exchange factor 7 |
UniProt Synonym Protein Names: | Beta-Pix; COOL-1; PAK-interacting exchange factor beta; p85 |
Protein Family: | Rho guanine nucleotide exchange factor |
UniProt Gene Name: | ARHGEF7 |
UniProt Entry Name: | ARHG7_HUMAN |