ARF1 Antibody (PACO17543)
제품 코드를 사용하여 Assay Genie 메인 사이트를 통해 주문합니다.
주문- SKU:
- PACO17543
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Description
Antibody Name: | ARF1 Antibody (PACO17543) |
Antibody SKU: | PACO17543 |
Size: | 50ul |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB, IHC |
Recommended Dilutions: | ELISA:1:1000-1:5000, WB:1:500-1:2000, IHC:1:25-1:100 |
Species Reactivity: | Human, Mouse, Rat |
Immunogen: | Synthetic peptide of human ARF1 |
Form: | Liquid |
Storage Buffer: | -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol |
Purification Method: | Antigen affinity purification |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
Gel: 12%SDS-PAGE, Lysate: 40 μg, Lane 1-2: Human fetal brain tissue, Human kidney tissue, Primary antibody: PACO17543(ARF1 Antibody) at dilution 1/500, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute. | |
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using PACO17543(ARF1 Antibody) at dilution 1/25, on the right is treated with synthetic peptide. (Original magnification: x200). |
Background: | ADP-ribosylation factor 1 (ARF1) is a member of the human ARF gene family. The family members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking as activators of phospholipase D. The gene products, including 6 ARF proteins and 11 ARF-like proteins, constitute a family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2 and ARF3), class II (ARF4 and ARF5) and class III (ARF6), and members of each class share a common gene organization. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. |
Synonyms: | ADP-ribosylation factor 1 |
UniProt Protein Function: | ARF1: GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP- ribosyltransferase. Involved in protein trafficking among different compartments. Modulates vesicle budding and uncoating within the Golgi complex. Deactivation induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, suggesting a crucial role in protein trafficking. In its GTP-bound form, its triggers the association with coat proteins with the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles. Belongs to the small GTPase superfamily. Arf family. |
UniProt Protein Details: | Protein type:G protein; G protein, monomeric, ARF; Motility/polarity/chemotaxis; G protein, monomeric Chromosomal Location of Human Ortholog: 1q42 Cellular Component: Golgi membrane; postsynaptic membrane; neuron projection; sarcomere; focal adhesion; perinuclear region of cytoplasm; postsynaptic density; plasma membrane; cytosol Molecular Function:GTPase activity; protein binding; GTP binding; receptor signaling protein activity Biological Process: protein transport; cellular copper ion homeostasis; COPI coating of Golgi vesicle; viral reproduction; regulation of receptor internalization; small GTPase mediated signal transduction; phospholipid metabolic process; phosphatidylinositol biosynthetic process; retrograde vesicle-mediated transport, Golgi to ER; antigen processing and presentation of exogenous peptide antigen via MHC class II; post-Golgi vesicle-mediated transport; regulation of defense response to virus by virus |
NCBI Summary: | ADP-ribosylation factor 1 (ARF1) is a member of the human ARF gene family. The family members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking as activators of phospholipase D. The gene products, including 6 ARF proteins and 11 ARF-like proteins, constitute a family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2 and ARF3), class II (ARF4 and ARF5) and class III (ARF6), and members of each class share a common gene organization. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008] |
UniProt Code: | P84077 |
NCBI GenInfo Identifier: | 51316985 |
NCBI Gene ID: | 375 |
NCBI Accession: | P84077.2 |
UniProt Secondary Accession: | P84077,P10947, P32889, |
UniProt Related Accession: | P84077 |
Molecular Weight: | 181 |
NCBI Full Name: | ADP-ribosylation factor 1 |
NCBI Synonym Full Names: | ADP-ribosylation factor 1 |
NCBI Official Symbol: | ARF1 |
NCBI Protein Information: | ADP-ribosylation factor 1 |
UniProt Protein Name: | ADP-ribosylation factor 1 |
Protein Family: | Auxin response factor |
UniProt Gene Name: | ARF1 |
UniProt Entry Name: | ARF1_HUMAN |
Antibodies |
Anti-ARF1 Antibody (CAB16345) |
Anti-ARF1 Antibody (CAB2821) |
Anti-ARF1 Antibody (CAB7643) |
Anti-ARF1 Antibody (CAB19977)[KO Validated] |
Anti-ARF1 Antibody (CAB9195) |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
Recommended Products |
Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
Anti-HRP-conjugated Beta Actin Antibody (CABC028) |