Anti-TPT1 Antibody (RACO0363)
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주문- SKU:
- RACO0363
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- IP
- Application:
- WB
- Application:
- IF
- Application:
- ELISA
- Application:
- IHC
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Product Name: | TPT1 Antibody |
Product SKU: | RACO0363 |
Size: | 50ul |
Host Species: | Homo sapiens (Human) |
Tested Applications: | ELISA, WB, IHC, IF, IP |
Recommended Dilutions: | WB:1:500-1:5000, IHC:1:50-1:200, IF:1:20-1:200, IP:1:200-1:1000 |
Species Reactivity: | Human, Mouse |
Immunogen: | A synthesized peptide derived from human TCTP |
Form: | Liquid |
Storage Buffer: | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Purification Method: | Affinity-chromatography |
Clonality: | Monoclonal |
Isotype: | Rabbit IgG |
Conjugate: | Non-conjugated |
Background: | Involved in calcium binding and microtubule stabilization. |
Synonyms: | Translationally-controlled tumor protein (TCTP) (Fortilin) (Histamine-releasing factor) (HRF) (p23), TPT1 |
IHC image of RACO0363 diluted at 1:100 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. | |
Immunofluorescence staining of SY5Y Cells with RACO0363 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L). | |
Western Blot. Positive WB detected in Hela whole cell lysate Jurkat whole cell lysate MCF-7 whole cell lysate RAW264.7 whole cell lysate HepG2 whole cell lysate PC-3 whole cell lysate Raji whole cell lysate 293 whole cell lysate Mouse liver tissue. All lanes TPT1 antibody at 1:2000. Secondary. Goat polyclonal to rabbit IgG at 1:50000 dilution. Predicted band size: 20, 16 kDa. Observed band size: 25 kDa. |
UniProt Protein Function: | TPT1: a novel heat shock protein with antiapoptotic and chaperone-like activity. Its levels are highly regulated in response to various cellular stimuli and stresses. Downregulated in response to proapoptotic treatments that induce Ca(++) stress, in a PKR-dependent manner. Colocalizes with CHFR to the mitotic spindle. Appears to be involved in calcium binding and microtubule stabilization. Its exosomal secretion is induced following DNA damage. Interacts with MCL1 and STEAP3, a p53-inducible transmembrane protein that facilitates the exosomal secretion of TPT1. Secreted TPT1 acts as a histamine-releasing factor, participates in inflammatory responses by inducing the release of histamine, and may play a role in tumor reversion. May play a direct role in placental calcium transport. Phosphorylation by Plk may decrease its microtubule-stabilizing activity and promote microtubule dynamics following metaphase. Critical for peripheral T cell maintenance and TCR-mediated cell proliferation. |
UniProt Protein Details: | Protein type:Calcium-binding; Microtubule-binding Chromosomal Location of Human Ortholog: 13q14 Cellular Component: multivesicular body; extracellular space; tubulin complex; cytoplasm; nucleus Molecular Function:protein binding; calcium ion binding Biological Process: regulation of apoptosis; cellular calcium ion homeostasis; response to virus; calcium ion transport; negative regulation of apoptosis |
UniProt Code: | P13693 |
NCBI GenInfo Identifier: | 136479 |
NCBI Gene ID: | 7178 |
NCBI Accession: | P13693.1 |
UniProt Secondary Accession: | P13693,Q6YLS2, Q7Z4J4, Q8TBK7, Q96EE2, Q9UC70, B2R7E5 |
UniProt Related Accession: | P13693 |
Molecular Weight: | 172 |
NCBI Full Name: | Translationally-controlled tumor protein |
NCBI Synonym Full Names: | tumor protein, translationally-controlled 1 |
NCBI Official Symbol: | TPT1Â Â |
NCBI Official Synonym Symbols: | HRF; p02; p23; TCTPÂ Â |
NCBI Protein Information: | translationally-controlled tumor protein; fortilin; histamine-releasing factor |
UniProt Protein Name: | Translationally-controlled tumor protein |
UniProt Synonym Protein Names: | Fortilin; Histamine-releasing factor; HRF; p23 |
Protein Family: | TPT1-like protein |
UniProt Gene Name: | TPT1Â Â |
UniProt Entry Name: | TCTP_HUMAN |