Anti-TOP2A Antibody (RACO0244)
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주문- SKU:
- RACO0244
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- WB
- Application:
- ELISA
- Application:
- IHC
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Product Name: | TOP2A Antibody |
Product SKU: | RACO0244 |
Size: | 50ul |
Host Species: | Homo sapiens (Human) |
Tested Applications: | ELISA, WB, IHC |
Recommended Dilutions: | WB:1:500-1:5000, IHC:1:50-1:200 |
Species Reactivity: | Human, Mouse |
Immunogen: | A synthesized peptide derived from human Topoisomerase II alpha |
Form: | Liquid |
Storage Buffer: | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Purification Method: | Affinity-chromatography |
Clonality: | Monoclonal |
Isotype: | Rabbit IgG |
Conjugate: | Non-conjugated |
Background: | Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Essential during mitosis and meiosis for proper segregation of daughter chromosomes. May play a role in regulating the period length of ARNTL/BMAL1 transcriptional oscillation (By similarity). |
Synonyms: | DNA topoisomerase 2-alpha (EC 5.99.1.3) (DNA topoisomerase II, alpha isozyme), TOP2A, TOP2 |
IHC image of RACO0244 diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. | |
Western Blot. Positive WB detected in Hela whole cell lysate Jurkat whole cell lysate K562 whole cell lysate NIH/3T3 whole cell lysate All lanes TOP2A antibody at 1:1500. Secondary. Goat polyclonal to rabbit IgG at 1:50000 dilution. Predicted band size: 175, 178, 179, 183 kDa. Observed band size: 175 kDa. |
UniProt Protein Function: | Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Essential during mitosis and meiosis for proper segregation of daughter chromosomes. May play a role in regulating the period length of ARNTL/BMAL1 transcriptional oscillation. |
NCBI Summary: | This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromosome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. [provided by RefSeq, Jul 2010] |
UniProt Code: | P11388 |
NCBI GenInfo Identifier: | 13959709 |
NCBI Gene ID: | 7153 |
NCBI Accession: | P11388.3 |
UniProt Secondary Accession: | P11388,Q71UN1, Q71UQ5, Q9HB24, Q9HB25, Q9HB26, Q9UP44 Q9UQP9, B2RTS1, |
UniProt Related Accession: | P11388 |
Molecular Weight: | 182,681 Da |
NCBI Full Name: | DNA topoisomerase 2-alpha |
NCBI Synonym Full Names: | topoisomerase (DNA) II alpha |
NCBI Official Symbol: | TOP2AÂ Â |
NCBI Official Synonym Symbols: | TOP2; TP2AÂ Â |
NCBI Protein Information: | DNA topoisomerase 2-alpha |
UniProt Protein Name: | DNA topoisomerase 2-alpha |
UniProt Synonym Protein Names: | DNA topoisomerase II, alpha isozyme |
Protein Family: | DNA topoisomerase |
UniProt Gene Name: | TOP2AÂ Â |
UniProt Entry Name: | TOP2A_HUMAN |