Anti-RIF1 Antibody (CAB15167)
- SKU:
- CAB15167
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Cycle
Frequently bought together:
Description
| Antibody Name: | Anti-RIF1 Antibody |
| Antibody SKU: | CAB15167 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 2173-2472 of human RIF1 (NP_060621.3). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human |
| Positive Samples: | U-87MG, HeLa, 293T, LO2 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 2173-2472 of human RIF1 (NP_060621.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | PLAS PSTS ILKR GLKR SQED EISS PVNK VRRV SFAD PIYQ AGLA DDID RRCS IVRS HSSN SSPI GKSV KTSP TTQS KHNT TSAK GFLS PGSR SPKF KSSK KCLI SEMA KESI PCPT ESVY PPLV NCVA PVDI ILPQ ITSN MWAR GLGQ LIRA KNIK TIGD LSTL TASE IKTL PIRS PKVS NVKK ALRI YHEQ QVKT RGLE EIPV FDIS EKTV NGIE NKSL SPDE ERLV SDII DPVA LEIP LSKN LLAQ ISAL ALQL DSED LHNY SGSQ LFEM HEKL SCMA NSVI KNLQ SRWR SPSH ENSI |
| Gene ID: | 55183 |
| Uniprot: | Q5UIP0 |
| Cellular Location: | Chromosome, Cytoplasm, Nucleus, cytoskeleton, spindle, telomere |
| Calculated MW: | 271kDa/274kDa |
| Observed MW: | 350kDa |
| Synonyms: | RIF1 |
| Background: | This gene encodes a protein that shares homology with the yeast teleomere binding protein, Rap1 interacting factor 1. This protein localizes to aberrant telomeres may be involved in DNA repair. Alternate splicing results in multiple transcript variants. |
| UniProt Protein Function: | RIF1: Required for checkpoint mediated arrest of cell cycle progression in response to DNA damage during S-phase (the intra-S- phase checkpoint). This checkpoint requires activation of at least 2 parallel pathways by the ATM kinase: one involves the MRN (MRE11A-RAD50-NBS1) complex, while the second requires CHEK2. RIF1 seems to act independently of both these pathways. Seems to play no role in either the G1/S or G2/M DNA damage checkpoints. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:DNA repair, damage Chromosomal Location of Human Ortholog: 2q23.3 Cellular Component: condensed chromosome; nucleoplasm; nucleus; plasma membrane; spindle midzone Biological Process: double-strand break repair via nonhomologous end joining; telomere maintenance in response to DNA damage |
| NCBI Summary: | This gene encodes a protein that shares homology with the yeast teleomere binding protein, Rap1 interacting factor 1. This protein localizes to aberrant telomeres may be involved in DNA repair. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Apr 2010] |
| UniProt Code: | Q5UIP0 |
| NCBI GenInfo Identifier: | 56676335 |
| NCBI Gene ID: | 55183 |
| NCBI Accession: | NP_060621.3 |
| UniProt Secondary Accession: | Q5UIP0,Q5H9R3, Q5UIP2, Q66YK6, Q6PRU2, Q8TE94, Q99772 Q9H830, Q9H9B9, Q9NVP5, A6NC27, C9JBR1, |
| UniProt Related Accession: | Q5UIP0 |
| Molecular Weight: | 271,689 Da |
| NCBI Full Name: | telomere-associated protein RIF1 isoform 1 |
| NCBI Synonym Full Names: | replication timing regulatory factor 1 |
| NCBI Official Symbol: | RIF1 |
| NCBI Protein Information: | telomere-associated protein RIF1 |
| UniProt Protein Name: | Telomere-associated protein RIF1 |
| UniProt Synonym Protein Names: | Rap1-interacting factor 1 homolog |
| Protein Family: | Telomere-associated protein |
| UniProt Gene Name: | RIF1 |
| UniProt Entry Name: | RIF1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using RIF1 antibody (CAB15167) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
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