Anti-POU2AF1 Antibody (RACO0395)
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주문- SKU:
- RACO0395
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- WB
- Application:
- ELISA
- Application:
- IHC
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Product Name: | POU2AF1 Antibody |
Product SKU: | RACO0395 |
Size: | 50ul |
Host Species: | Homo sapiens (Human) |
Tested Applications: | ELISA, WB, IHC |
Recommended Dilutions: | WB:1:500-1:5000, IHC:1:50-1:200 |
Species Reactivity: | Human |
Immunogen: | A synthesized peptide derived from human BOB1 |
Form: | Liquid |
Storage Buffer: | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Purification Method: | Affinity-chromatography |
Clonality: | Monoclonal |
Isotype: | Rabbit IgG |
Conjugate: | Non-conjugated |
Background: | Transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers. |
Synonyms: | POU domain class 2-associating factor 1 (B-cell-specific coactivator OBF-1) (BOB-1) (OCA-B) (OCT-binding factor 1), POU2AF1, OBF1 |
IHC image of RACO0395 diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. | |
IHC image of RACO0395 diluted at 1:100 and staining in paraffin-embedded human appendix tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. | |
Western Blot. Positive WB detected in Raji whole cell lysate All lanes BOB1 antibody at 1:2000. Secondary. Goat polyclonal to rabbit IgG at 1:50000 dilution. Predicted band size: 28 kDa. Observed band size: 35 kDa. |
UniProt Protein Function: | POU2AF1: Transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers. A chromosomal aberration involving POU2AF1/OBF1 may be a cause of a form of B-cell leukemia. Translocation t(3;11)(q27;q23) with BCL6. Belongs to the POU2AF1 family. |
UniProt Protein Details: | Protein type:Oncoprotein; Transcription, coactivator/corepressor Chromosomal Location of Human Ortholog: 11q23.1 Cellular Component: nucleus Molecular Function:protein binding; DNA binding; transcription coactivator activity; transcription cofactor activity Biological Process: transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; humoral immune response |
UniProt Code: | Q16633 |
NCBI GenInfo Identifier: | 2833276 |
NCBI Gene ID: | 5450 |
NCBI Accession: | Q16633.1 |
UniProt Secondary Accession: | Q16633,Q14983, B2R8Z9, |
UniProt Related Accession: | Q16633,AAB34937 |
Molecular Weight: | 256 |
NCBI Full Name: | POU domain class 2-associating factor 1 |
NCBI Synonym Full Names: | POU class 2 associating factor 1 |
NCBI Official Symbol: | POU2AF1Â Â |
NCBI Official Synonym Symbols: | BOB1; OBF1; OCAB; OBF-1Â Â |
NCBI Protein Information: | POU domain class 2-associating factor 1; BOB-1; OCA-B; OCT-binding factor 1; B-cell-specific coactivator OBF-1; POU domain, class 2, associating factor 1 |
UniProt Protein Name: | POU domain class 2-associating factor 1 |
UniProt Synonym Protein Names: | B-cell-specific coactivator OBF-1; BOB-1; OCA-B; OCT-binding factor 1 |
Protein Family: | POU domain class 2-associating factor |
UniProt Gene Name: | POU2AF1Â Â |
UniProt Entry Name: | OBF1_HUMAN |