Anti-PIK3CB Antibody (RACO0243)
제품 코드를 사용하여 Assay Genie 메인 사이트를 통해 주문합니다.
주문- SKU:
- RACO0243
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- IP
- Application:
- WB
- Application:
- ELISA
- Conjugation:
- Unconjugated
Description
Product Name: | PIK3CB Antibody |
Product SKU: | RACO0243 |
Size: | 50ul |
Host Species: | Homo sapiens (Human) |
Tested Applications: | ELISA, WB, IP |
Recommended Dilutions: | WB:1:500-1:5000, IP:1:200-1:1000 |
Species Reactivity: | Human |
Immunogen: | A synthesized peptide derived from human PI3 Kinase p110 beta |
Form: | Liquid |
Storage Buffer: | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Purification Method: | Affinity-chromatography |
Clonality: | Monoclonal |
Isotype: | Rabbit IgG |
Conjugate: | Non-conjugated |
Background: | Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4-phosphate) and PtdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Involved in the activation of AKT1 upon stimulation by G-protein coupled receptors (GPCRs) ligands such as CXCL12, sphingosine 1-phosphate, and lysophosphatidic acid. May also act downstream receptor tyrosine kinases. Required in different signaling pathways for stable platelet adhesion and aggregation. Plays a role in platelet activation signaling triggered by GPCRs, alpha-IIb/beta-3 integrins (ITGA2B/ ITGB3) and ITAM (immunoreceptor tyrosine-based activation motif)-bearing receptors such as GP6. Regulates the strength of adhesion of ITGA2B/ ITGB3 activated receptors necessary for the cellular transmission of contractile forces. Required for platelet aggregation induced by F2 (thrombin) and thromboxane A2 (TXA2). Has a role in cell survival. May have a role in cell migration. Involved in the early stage of autophagosome formation. Modulates the intracellular level of PtdIns3P (Phosphatidylinositol 3-phosphate) and activates PIK3C3 kinase activity. May act as a scaffold, independently of its lipid kinase activity to positively regulate autophagy. May have a role in insulin signaling as scaffolding protein in which the lipid kinase activity is not required. May have a kinase-independent function in regulating cell proliferation and in clathrin-mediated endocytosis. Mediator of oncogenic signal in cell lines lacking PTEN. The lipid kinase activity is necessary for its role in oncogenic transformation. Required for the growth of ERBB2 and RAS driven tumors. |
Synonyms: | Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform (PI3-kinase subunit beta) (PI3K-beta) (PI3Kbeta) (PtdIns-3-kinase subunit beta) (EC 2.7.1.153) (Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit beta) (PtdIns-3-kinase subunit p110-beta) (p110beta), PIK3CB, PIK3C1 |
Immunoprecipitating PIK3CB in K562 whole cell lysate Lane 1: Rabbit control IgG instead of RACO0243 in K562 whole cell lysate For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: RACO0243(2µg)+ K562 whole cell lysate (500µg). Lane 3: K562 whole cell lysate (10µg). | |
Western Blot. Positive WB detected in Hela whole cell lysate K562 whole cell lysate All lanes PIK3CB antibody at 1:1500. Secondary. Goat polyclonal to rabbit IgG at 1:50000 dilution. Predicted band size: 123 kDa. Observed band size: 110 kDa. |
UniProt Protein Function: | PIK3CB: catalytic subunit of phosphoinositide-3-kinase beta. Catalyzes the production of phosphatidylinositol-3,4,5-triphosphate by phosphorylating phosphatidylinositol (PI), phosphatidylinositol-4-phosphate (PIP) and phosphatidylinositol-4,5-bisphosphate (PIP2). Growth factors and hormones trigger this phosphorylation event, which in turn coordinates cell growth, cell cycle entry, cell migration and cell survival. PTEN reverses this process, and the PI3K signaling pathway is constitutively activated in human cancers that have loss of function of PTEN. Expressed ubiquitously. |
UniProt Protein Details: | Protein type:Carbohydrate Metabolism - inositol phosphate; Motility/polarity/chemotaxis; EC 2.7.1.153; Kinase, lipid Chromosomal Location of Human Ortholog: 3q22.3 Cellular Component: cytosol; intercellular bridge; nucleolus; nucleus; phosphoinositide 3-kinase complex; plasma membrane Molecular Function:1-phosphatidylinositol-3-kinase activity; phosphatidylinositol-4,5-bisphosphate 3-kinase activity; phosphatidylinositol-4-phosphate 3-kinase activity; phosphoinositide 3-kinase activity; protein binding Biological Process: activation of MAPK activity; cell migration; chemotaxis; G-protein coupled receptor protein signaling pathway; leukocyte migration; phosphatidylinositol biosynthetic process; phosphoinositide 3-kinase cascade; phosphoinositide-mediated signaling; platelet activation; positive regulation of autophagy; regulation of cell-matrix adhesion; regulation of phosphoinositide 3-kinase cascade; T cell receptor signaling pathway; transmembrane receptor protein tyrosine kinase signaling pathway; vascular endothelial growth factor receptor signaling pathway |
NCBI Summary: | This gene encodes an isoform of the catalytic subunit of phosphoinositide 3-kinase (PI3K). These kinases are important in signaling pathways involving receptors on the outer membrane of eukaryotic cells and are named for their catalytic subunit. The encoded protein is the catalytic subunit for PI3Kbeta (PI3KB). PI3KB has been shown to be part of the activation pathway in neutrophils which have bound immune complexes at sites of injury or infection. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2011] |
UniProt Code: | P42338 |
NCBI GenInfo Identifier: | 1171955 |
NCBI Gene ID: | 5291 |
NCBI Accession: | P42338.1 |
UniProt Secondary Accession: | P42338,Q24JU2, D3DNF0, |
UniProt Related Accession: | P42338 |
Molecular Weight: | 122,762 Da |
NCBI Full Name: | Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform |
NCBI Synonym Full Names: | phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit beta |
NCBI Official Symbol: | PIK3CBÂ Â |
NCBI Official Synonym Symbols: | PI3K; PIK3C1; P110BETA; PI3KBETAÂ Â |
NCBI Protein Information: | phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform |
UniProt Protein Name: | Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform |
UniProt Synonym Protein Names: | Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit beta; PtdIns-3-kinase subunit p110-beta; p110beta |
Protein Family: | Phosphatidylinositol 4,5-bisphosphate 3-kinase |
UniProt Gene Name: | PIK3CBÂ Â |
UniProt Entry Name: | PK3CB_HUMAN |