Anti-Phospho-PAK1/PAK2/PAK3 (S144+S141+S139) Antibody (RACO0069)

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SKU:
RACO0069
Product type:
Recombinant Antibody
Reactivity:
Human
Host Species:
Human
Isotype:
IgG
Application:
WB
Application:
ELISA
Application:
IHC
Conjugation:
Unconjugated
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Description

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Product Name:Phospho-PAK1/PAK2/PAK3 (S144+S141+S139) Antibody
Product SKU:RACO0069
Size:50ul
Host Species:Human
Tested Applications:ELISA, WB, IHC
Recommended Dilutions:WB:1:500-1:5000, IHC:1:50-1:200
Species Reactivity:Human
Immunogen:A synthesized peptide derived from human Phospho-PAK1/PAK2/PAK3 (S144+S141+S139)
Form:Liquid
Storage Buffer:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:Affinity-chromatography
Clonality:Monoclonal
Isotype:Rabbit IgG
Conjugate:Non-conjugated
Background:Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, or cell cycle regulation. Plays a role in dendrite spine morphogenesis as well as synapse formation and plasticity. Acts as downstream effector of the small GTPases CDC42 and RAC1. Activation by the binding of active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates MAPK4 and MAPK6 and activates the downstream target MAPKAPK5, a regulator of F-actin polymerization and cell migration. Additionally, phosphorylates TNNI3/troponin I to modulate calcium sensitivity and relaxation kinetics of thin myofilaments. May also be involved in early neuronal development.
Synonyms:Serine/threonine-protein kinase PAK 3, Beta-PAK, Oligophrenin-3, p21-activated kinase 3, PAK-3, PAK3, OPHN3
Product ImageIHC image of RACO0069 diluted at 1:100 and staining in paraffin-embedded rat brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Product ImageWestern Blot. Positive WB detected in A549 whole cell lysate treated with EGF or not). All lanes Phospho-PAK1/PAK2/PAK3 antibody at 1(2µg)ml. Secondary. Goat polyclonal to rabbit IgG at 1:50000 dilution. Predicted band size: 65 KDa. Observed band size: 65 KDa.
UniProt Protein Function:PAK3: a protein kinase of the STE20 family that regulates synapse formation and plasticity in the hippocampus. May be necessary for dendritic development and for the rapid cytoskeletal reorganization in dendritic spines associated with synaptic plasticity. Forms an activated complex with GTP-bound P21, CDC2 and RAC1 proteins. Missense and truncation mutations linked to nonsyndromic mental retardation type 30 (MRX30). Two alternatively spliced human isoforms have been reported.
UniProt Protein Details:

Protein type:Protein kinase, STE; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.1; STE group; STE20 family; PAKA subfamily

Chromosomal Location of Human Ortholog: Xq23

Cellular Component: cytoplasm; cytosol; endosome; plasma membrane

Molecular Function:ATP binding; MAP kinase kinase activity; metal ion binding; protein binding; protein serine/threonine kinase activity; Rac GTPase binding; SH3 domain binding

Biological Process: actin cytoskeleton organization and biogenesis; activation of MAPK activity; axon guidance; axonogenesis; cell migration; dendrite development; ephrin receptor signaling pathway; innate immune response; MAPKKK cascade; positive regulation of neuron apoptosis; regulation of actin filament polymerization; regulation of apoptosis; regulation of mitotic cell cycle; Rho protein signal transduction; small GTPase mediated signal transduction; stimulatory C-type lectin receptor signaling pathway; stress-activated protein kinase signaling pathway; synapse organization and biogenesis; T cell costimulation; T cell receptor signaling pathway; vascular endothelial growth factor receptor signaling pathway

Disease: Mental Retardation, X-linked 30

NCBI Summary:PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. PAK proteins, a family of serine/threonine p21-activating kinases, serve as targets for the small GTP binding proteins Cdc42 and RAC and have been implicated in a wide range of biological activities. The protein encoded by this gene forms an activated complex with GTP-bound RAS-like (P21), CDC2 and RAC1 proteins which then catalyzes a variety of targets. This protein may be necessary for dendritic development and for the rapid cytoskeletal reorganization in dendritic spines associated with synaptic plasticity. Defects in this gene are the cause of non-syndromic mental retardation X-linked type 30 (MRX30), also called X-linked mental retardation type 47 (MRX47). Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
UniProt Code:O75914
NCBI GenInfo Identifier:47117818
NCBI Gene ID:5063
NCBI Accession:O75914.2
UniProt Secondary Accession:O75914,Q5JWX1, Q5JWX2, Q7Z2D6, Q7Z2E4, Q7Z3Z8, Q8WWK5 Q8WX23, A8K389, B1GX77, B1GX78, B1GX79,
UniProt Related Accession:O75914
Molecular Weight:62,913 Da
NCBI Full Name:Serine/threonine-protein kinase PAK 3
NCBI Synonym Full Names:p21 protein (Cdc42/Rac)-activated kinase 3
NCBI Official Symbol:PAK3  
NCBI Official Synonym Symbols:bPAK; MRX30; MRX47; OPHN3; PAK-3; PAK3beta; beta-PAK  
NCBI Protein Information:serine/threonine-protein kinase PAK 3
UniProt Protein Name:Serine/threonine-protein kinase PAK 3
UniProt Synonym Protein Names:Beta-PAK; Oligophrenin-3; p21-activated kinase 3; PAK-3
Protein Family:Serine/threonine-protein kinase
UniProt Gene Name:PAK3  
UniProt Entry Name:PAK3_HUMAN
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