Anti-Phospho-LAT (Y191) Antibody (RACO0125)
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주문- SKU:
- RACO0125
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- IP
- Application:
- WB
- Application:
- IF
- Application:
- ELISA
- Application:
- IHC
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Product Name: | Phospho-LAT (Y191) Antibody |
Product SKU: | RACO0125 |
Size: | 50ul |
Host Species: | Human |
Tested Applications: | ELISA, WB, IHC, IF, IP |
Recommended Dilutions: | WB:1:500-1:5000, IHC:1:50-1:200, IF:1:20-1:200, IP:1:200-1:1000 |
Species Reactivity: | Human |
Immunogen: | A synthesized peptide derived from human Phospho-LAT (Y191) |
Form: | Liquid |
Storage Buffer: | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Purification Method: | Affinity-chromatography |
Clonality: | Monoclonal |
Isotype: | Rabbit IgG |
Conjugate: | Non-conjugated |
Background: | Required for TCR (T-cell antigen receptor)- and pre-TCR-mediated signaling, both in mature T-cells and during their development. Involved in FCGR3 (low affinity immunoglobulin gamma Fc region receptor III)-mediated signaling in natural killer cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Couples activation of these receptors and their associated kinases with distal intracellular events such as mobilization of intracellular calcium stores, PKC activation, MAPK activation or cytoskeletal reorganization through the recruitment of PLCG1, GRB2, GRAP2, and other signaling molecules. |
Synonyms: | Linker for activation of T-cells family member 1, 36 kDa phospho-tyrosine adapter protein, pp36, p36-38, LAT |
IHC image of RACO0125 diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. | |
IHC image of RACO0125 diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. | |
Western Blot. Positive WB detected in Jurkat whole cell lysate Hela whole cell lysate HepG2 whole cell lysate treated with EGF or Pervanadate) . All lanes Phospho-LAT antibody at 2.9µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1:50000 dilution. Predicted band size: 38 KDa. Observed band size: 38 KDa. |
UniProt Protein Function: | Required for TCR (T-cell antigen receptor)- and pre-TCR-mediated signaling, both in mature T-cells and during their development. Involved in FCGR3 (low affinity immunoglobulin gamma Fc region receptor III)-mediated signaling in natural killer cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Couples activation of these receptors and their associated kinases with distal intracellular events such as mobilization of intracellular calcium stores, PKC activation, MAPK activation or cytoskeletal reorganization through the recruitment of PLCG1, GRB2, GRAP2, and other signaling molecules. |
NCBI Summary: | The protein encoded by this gene is phosphorylated by ZAP-70/Syk protein tyrosine kinases following activation of the T-cell antigen receptor (TCR) signal transduction pathway. This transmembrane protein localizes to lipid rafts and acts as a docking site for SH2 domain-containing proteins. Upon phosphorylation, this protein recruits multiple adaptor proteins and downstream signaling molecules into multimolecular signaling complexes located near the site of TCR engagement. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] |
UniProt Code: | O43561 |
NCBI GenInfo Identifier: | 14194891 |
NCBI Gene ID: | 27040 |
NCBI Accession: | O43561.1 |
UniProt Secondary Accession: | O43561,O43919, B7WPI0, C7C5T6, G5E9K3, |
UniProt Related Accession: | O43561 |
Molecular Weight: | 27,774 Da |
NCBI Full Name: | Linker for activation of T-cells family member 1 |
NCBI Synonym Full Names: | linker for activation of T-cells |
NCBI Official Symbol: | LATÂ Â |
NCBI Official Synonym Symbols: | LAT1; pp36Â Â |
NCBI Protein Information: | linker for activation of T-cells family member 1 |
UniProt Protein Name: | Linker for activation of T-cells family member 1 |
UniProt Synonym Protein Names: | 36 kDa phospho-tyrosine adapter protein; pp36; p36-38 |
Protein Family: | Latexin |
UniProt Gene Name: | LATÂ Â |
UniProt Entry Name: | LAT_HUMAN |