Anti-PDCD6IP Antibody (RACO0423)
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주문- SKU:
- RACO0423
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Reactivity:
- Rat
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- WB
- Application:
- ELISA
- Application:
- FC
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Product Name: | PDCD6IP Antibody |
Product SKU: | RACO0423 |
Size: | 50ul |
Host Species: | Homo sapiens (Human) |
Tested Applications: | ELISA, WB, FC |
Recommended Dilutions: | WB:1:500-1:5000, FC:1:20-1:200 |
Species Reactivity: | Human, Rat |
Immunogen: | A synthesized peptide derived from human ALIX |
Form: | Liquid |
Storage Buffer: | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Purification Method: | Affinity-chromatography |
Clonality: | Monoclonal |
Isotype: | Rabbit IgG |
Conjugate: | Non-conjugated |
Background: | Multifunctional protein involved in endocytosis, multivesicular body biogenesis, membrane repair, cytokinesis, apoptosis and maintenance of tight junction integrity. Class E VPS protein involved in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome. Binds to the phospholipid lysobisphosphatidic acid (LBPA) which is abundant in MVBs internal membranes. The MVB pathway requires the sequential function of ESCRT-O, -I,-II and -III complexes (PubMed:14739459). The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis (PubMed:17853893, PubMed:17556548). Adapter for a subset of ESCRT-III proteins, such as CHMP4, to function at distinct membranes. Required for completion of cytokinesis (PubMed:17853893, PubMed:17556548, PubMed:18641129). May play a role in the regulation of both apoptosis and cell proliferation. Regulates exosome biogenesis in concert with SDC1/4 and SDCBP (PubMed:22660413). By interacting with F-actin, PARD3 and TJP1 secures the proper assembly and positioning of actomyosin-tight junction complex at the apical sides of adjacent epithelial cells that defines a spatial membrane domain essential for the maintenance of epithelial cell polarity and barrier (By similarity). |
Synonyms: | Programmed cell death 6-interacting protein (PDCD6-interacting protein) (ALG-2-interacting protein 1) (ALG-2-interacting protein X) (Hp95), PDCD6IP, AIP1 ALIX KIAA1375 |
Overlay histogram showing Jurkat cells stained with RACO0423 (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1µg) 1*106cells) for 1 h at 4°C.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4°C. Control antibody (green line) was Rabbit IgG (1µg) 1*106cells) used under the same conditions. Acquisition of >10,000 events was performed. | |
Western Blot. Positive WB detected in L02 whole cell lysate HepG2 whole cell lysate PC-3 whole cell lysate U-87 whole cell lysate Rat Liver whole cell lysate All lanes ALIX antibody at 1:1000. Secondary. Goat polyclonal to rabbit IgG at 1:50000 dilution. Predicted band size: 97, 97, 31 kDa. Observed band size: 97 kDa. |
UniProt Protein Function: | Alix: an adaptor protein that may regulate the function of receptor and cytoskeleton-associated tyrosine kinases. A class E vacuolar protein sorting (VPS) factor involved in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles. Fusion between endosomes and the vacuole will then target the cargo proteins to the vacuolar lumen. In case of infection, the HIV-1 virus directly takes advantage of its function for viral exocytosis and budding, via its interaction with HIV-1 p6 protein. May play a role in the regulation of both apoptosis and cell proliferation. |
UniProt Protein Details: | Protein type:Apoptosis; Adaptor/scaffold Chromosomal Location of Human Ortholog: 3p22.3 Cellular Component: focal adhesion; membrane; cytoplasm; melanosome; microtubule organizing center; immunological synapse; cytosol; vesicle Molecular Function:protein binding; protein homodimerization activity; proteinase activated receptor binding; SH3 domain binding; calcium-dependent protein binding Biological Process: protein transport; cell separation during cytokinesis; viral reproduction; apoptosis; viral infectious cycle; mitotic metaphase plate congression; nuclear organization and biogenesis |
NCBI Summary: | This gene encodes a protein that functions within the ESCRT pathway in the abscission stage of cytokinesis, in intralumenal endosomal vesicle formation, and in enveloped virus budding. Studies using mouse cells have shown that overexpression of this protein can block apoptosis. In addition, the product of this gene binds to the product of the PDCD6 gene, a protein required for apoptosis, in a calcium-dependent manner. This gene product also binds to endophilins, proteins that regulate membrane shape during endocytosis. Overexpression of this gene product and endophilins results in cytoplasmic vacuolization, which may be partly responsible for the protection against cell death. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. Related pseudogenes have been identified on chromosome 15. [provided by RefSeq, Jan 2012] |
UniProt Code: | Q8WUM4 |
NCBI GenInfo Identifier: | 31076831 |
NCBI Gene ID: | 10015 |
NCBI Accession: | Q8WUM4.1 |
UniProt Secondary Accession: | Q8WUM4,Q6NUS1, Q9BX86, Q9NUN0, Q9P2H2, Q9UKL5, C5MQH7 E9PFU1, |
UniProt Related Accession: | Q8WUM4 |
Molecular Weight: | 868 |
NCBI Full Name: | Programmed cell death 6-interacting protein |
NCBI Synonym Full Names: | programmed cell death 6 interacting protein |
NCBI Official Symbol: | PDCD6IPÂ Â |
NCBI Official Synonym Symbols: | AIP1; ALIX; HP95; DRIP4Â Â |
NCBI Protein Information: | programmed cell death 6-interacting protein; PDCD6-interacting protein; ALG-2 interacting protein 1; ALG-2-interacting protein X; dopamine receptor interacting protein 4; apoptosis-linked gene 2-interacting protein X |
UniProt Protein Name: | Programmed cell death 6-interacting protein |
UniProt Synonym Protein Names: | ALG-2-interacting protein 1; ALG-2-interacting protein X; Hp95 |
Protein Family: | Programmed cell death 6-interacting protein |
UniProt Gene Name: | PDCD6IPÂ Â |
UniProt Entry Name: | PDC6I_HUMAN |