Anti-PAK1 Antibody (RACO0400)
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주문- SKU:
- RACO0400
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- WB
- Application:
- IF
- Application:
- ELISA
- Conjugation:
- Unconjugated
Description
Product Name: | PAK1 Antibody |
Product SKU: | RACO0400 |
Size: | 50ul |
Host Species: | Homo sapiens (Human) |
Tested Applications: | ELISA, WB, IF |
Recommended Dilutions: | WB:1:500-1:5000, IF:1:20-1:200 |
Species Reactivity: | Human, Mouse |
Immunogen: | A synthesized peptide derived from human PAK1 |
Form: | Liquid |
Storage Buffer: | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Purification Method: | Affinity-chromatography |
Clonality: | Monoclonal |
Isotype: | Rabbit IgG |
Conjugate: | Non-conjugated |
Background: | Protein kinase involved in intracellular signaling pathways downstream of integrins and receptor-type kinases that plays an important role in cytoskeleton dynamics, in cell adhesion, migration, proliferation, apoptosis, mitosis, and in vesicle-mediated transport processes. Can directly phosphorylate BAD and protects cells against apoptosis. Activated by interaction with CDC42 and RAC1. Functions as GTPase effector that links the Rho-related GTPases CDC42 and RAC1 to the JNK MAP kinase pathway. Phosphorylates and activates MAP2K1, and thereby mediates activation of downstream MAP kinases. Involved in the reorganization of the actin cytoskeleton, actin stress fibers and of focal adhesion complexes. Phosphorylates the tubulin chaperone TBCB and thereby plays a role in the regulation of microtubule biogenesis and organization of the tubulin cytoskeleton. Plays a role in the regulation of insulin secretion in response to elevated glucose levels. Part of a ternary complex that contains PAK1, DVL1 and MUSK that is important for MUSK-dependent regulation of AChR clustering during the formation of the neuromuscular junction (NMJ). Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2. Phosphorylates MYL9/MLC2. Phosphorylates RAF1 at 'Ser-338' and 'Ser-339' resulting in: activation of RAF1, stimulation of RAF1 translocation to mitochondria, phosphorylation of BAD by RAF1, and RAF1 binding to BCL2. Phosphorylates SNAI1 at 'Ser-246' promoting its transcriptional repressor activity by increasing its accumulation in the nucleus. In podocytes, promotes NR3C2 nuclear localization. Required for atypical chemokine receptor ACKR2-induced phosphorylation of LIMK1 and cofilin (CFL1) and for the up-regulation of ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. In synapses, seems to mediate the regulation of F-actin cluster formation performed by SHANK3, maybe through CFL1 phosphorylation and inactivation. Plays a role in RUFY3-mediated facilitating gastric cancer cells migration and invasion (PubMed:25766321). |
Synonyms: | Serine/threonine-protein kinase PAK 1 (EC 2.7.11.1) (Alpha-PAK) (p21-activated kinase 1) (PAK-1) (p65-PAK), PAK1 |
Immunofluorescence staining of Hela Cells with RACO0400 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L). | |
Western Blot. Positive WB detected in NIH/3T3 whole cell lysate MCF-7 whole cell lysate ntera-2 whole cell lysate Mouse brain tissue. All lanes PAK1 antibody at 1:2000. Secondary. Goat polyclonal to rabbit IgG at 1:50000 dilution. Predicted band size: 61, 62 kDa. Observed band size: 61 kDa. |
UniProt Protein Function: | PAK1: a protein kinase of the STE20 family that regulates cell motility and morphology. Critical RhoGTPase effector that regulates cytoskeleton reorganization, the JNK MAPK pathway, and nuclear signaling. Binding of Rac/cdc42 to the PBD of PAK1 causes autophosphorylation and conformational change. Phosphorylated and activated by PDK. |
UniProt Protein Details: | Protein type:Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.1; Protein kinase, STE; STE group; STE20 family; PAKA subfamily Chromosomal Location of Human Ortholog: 11q13-q14 Cellular Component: axon; cytoplasm; cytosol; dendrite; filamentous actin; focal adhesion; Golgi apparatus; growth cone; intercellular junction; nuclear membrane; plasma membrane; protein complex; ruffle; Z disc Molecular Function:ATP binding; collagen binding; protein binding; protein kinase activity; protein kinase binding; protein serine/threonine kinase activity; Rac GTPase binding Biological Process: actin cytoskeleton reorganization; activation of protein kinase activity; amygdala development; apoptosis; axon guidance; branching morphogenesis of a tube; cell migration; cellular response to insulin stimulus; ephrin receptor signaling pathway; exocytosis; innate immune response; MAPKKK cascade; neurite morphogenesis; neuromuscular junction development; positive regulation of cell migration; positive regulation of estrogen receptor signaling pathway; positive regulation of JNK activity; positive regulation of peptidyl-serine phosphorylation; positive regulation of protein amino acid phosphorylation; positive regulation of stress fiber formation; protein amino acid autophosphorylation; protein amino acid phosphorylation; receptor clustering; regulation of apoptosis; regulation of gene expression; regulation of mitotic cell cycle; response to hypoxia; Rho protein signal transduction; small GTPase mediated signal transduction; stimulatory C-type lectin receptor signaling pathway; stress-activated protein kinase signaling pathway; T cell costimulation; T cell receptor signaling pathway; vascular endothelial growth factor receptor signaling pathway; wound healing |
NCBI Summary: | This gene encodes a family member of serine/threonine p21-activating kinases, known as PAK proteins. These proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling, and they serve as targets for the small GTP binding proteins Cdc42 and Rac. This specific family member regulates cell motility and morphology. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2010] |
UniProt Code: | Q13153 |
NCBI GenInfo Identifier: | 90111767 |
NCBI Gene ID: | 5058 |
NCBI Accession: | Q13153.2 |
UniProt Secondary Accession: | Q13153,O75561, Q13567, Q32M53, Q32M54, Q86W79, |
UniProt Related Accession: | Q13153 |
Molecular Weight: | |
NCBI Full Name: | Serine/threonine-protein kinase PAK 1 |
NCBI Synonym Full Names: | p21 protein (Cdc42/Rac)-activated kinase 1 |
NCBI Official Symbol: | PAK1Â Â |
NCBI Official Synonym Symbols: | PAKalpha  |
NCBI Protein Information: | serine/threonine-protein kinase PAK 1 |
UniProt Protein Name: | Serine/threonine-protein kinase PAK 1 |
UniProt Synonym Protein Names: | Alpha-PAK; p21-activated kinase 1; PAK-1; p65-PAK |
Protein Family: | Serine/threonine-protein kinase |
UniProt Gene Name: | PAK1Â Â |
UniProt Entry Name: | PAK1_HUMAN |