Anti-PABPN1 Antibody (RACO0333)
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주문- SKU:
- RACO0333
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- WB
- Application:
- IF
- Application:
- ELISA
- Application:
- IHC
- Application:
- FC
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Product Name: | PABPN1 Antibody |
Product SKU: | RACO0333 |
Size: | 50ul |
Host Species: | Homo sapiens (Human) |
Tested Applications: | ELISA, WB, IHC, IF, FC |
Recommended Dilutions: | WB:1:500-1:5000, IHC:1:50-1:200, IF:1:20-1:200, FC:1:20-1:200 |
Species Reactivity: | Human |
Immunogen: | A synthesized peptide derived from human PABPN1 |
Form: | Liquid |
Storage Buffer: | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Purification Method: | Affinity-chromatography |
Clonality: | Monoclonal |
Isotype: | Rabbit IgG |
Conjugate: | Non-conjugated |
Background: | Involved in the 3'-end formation of mRNA precursors (pre-mRNA) by the addition of a poly(A) tail of 200-250 nt to the upstream cleavage product (By similarity). Stimulates poly(A) polymerase (PAPOLA) conferring processivity on the poly(A) tail elongation reaction and controls also the poly(A) tail length (By similarity). Increases the affinity of poly(A) polymerase for RNA (By similarity). Is also present at various stages of mRNA metabolism including nucleocytoplasmic trafficking and nonsense-mediated decay (NMD) of mRNA. Cooperates with SKIP to synergistically activate E-box-mediated transcription through MYOD1 and may regulate the expression of muscle-specific genes (PubMed:11371506). Binds to poly(A) and to poly(G) with high affinity (By similarity). May protect the poly(A) tail from degradation (By similarity). |
Synonyms: | Polyadenylate-binding protein 2 (PABP-2) (Poly(A)-binding protein 2) (Nuclear poly(A)-binding protein 1) (Poly(A)-binding protein II) (PABII) (Polyadenylate-binding nuclear protein 1), PABPN1, PAB2 PABP2 |
IHC image of RACO0333 diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. | |
IHC image of RACO0333 diluted at 1:100 and staining in paraffin-embedded human bladder cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. | |
Western Blot. Positive WB detected in 293 whole cell lysate MCF-7 whole cell lysate Raji whole cell lysate HepG2 whole cell lysate All lanes PABPN1 antibody at 1:2000. Secondary. Goat polyclonal to rabbit IgG at 1:50000 dilution. Predicted band size: 33, 32, 38 kDa. Observed band size: 50 kDa. |
UniProt Code: | Q86U42 |
NCBI GenInfo Identifier: | 46403176 |
NCBI Gene ID: | 8106 |
NCBI Accession: | Q86U42.3 |
UniProt Secondary Accession: | Q86U42,O43484, D3DS49, |
UniProt Related Accession: | Q86U42 |
Molecular Weight: | 306 |
NCBI Full Name: | Polyadenylate-binding protein 2 |
NCBI Synonym Full Names: | poly(A) binding protein, nuclear 1 |
NCBI Official Symbol: | PABPN1Â Â |
NCBI Official Synonym Symbols: | OPMD; PAB2; PABII; PABP2; PABP-2Â Â |
NCBI Protein Information: | polyadenylate-binding protein 2; poly(A) binding protein 2; poly(A) binding protein II |
UniProt Protein Name: | Polyadenylate-binding protein 2 |
UniProt Synonym Protein Names: | Nuclear poly(A)-binding protein 1; Poly(A)-binding protein II; PABII; Polyadenylate-binding nuclear protein 1 |
UniProt Gene Name: | PABPN1Â Â |
UniProt Entry Name: | PABP2_HUMAN |