Anti-NUP98 Antibody (RACO0466)
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주문- SKU:
- RACO0466
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- WB
- Application:
- IF
- Application:
- ELISA
- Application:
- IHC
- Conjugation:
- Unconjugated
Description
Product Name: | NUP98 Antibody |
Product SKU: | RACO0466 |
Size: | 50ul |
Host Species: | Homo sapiens (Human) |
Tested Applications: | ELISA, WB, IHC, IF |
Recommended Dilutions: | WB:1:500-1:5000, IHC:1:50-1:200, IF:1:20-1:200 |
Species Reactivity: | Human |
Immunogen: | A synthesized peptide derived from human NUP98 |
Form: | Liquid |
Storage Buffer: | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Purification Method: | Affinity-chromatography |
Clonality: | Monoclonal |
Isotype: | Rabbit IgG |
Conjugate: | Non-conjugated |
Background: | Plays a role in the nuclear pore complex (NPC) assembly and/or maintenance. Nup98 and Nup96 are involved in the bidirectional transport across the NPC. May anchor NUP153 and TPR to the NPC. |
Synonyms: | Nuclear pore complex protein Nup98-Nup96 (EC 3.4.21.-) [Cleaved into: Nuclear pore complex protein Nup98 (98 kDa nucleoporin) (Nucleoporin Nup98) (Nup98), Nuclear pore complex protein Nup96 (96 kDa nucleoporin) (Nucleoporin Nup96) (Nup96)], NUP98, ADAR2 |
IHC image of RACO0466 diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. | |
Immunofluorescence staining of Hela Cells with RACO0466 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L). | |
Western Blot. Positive WB detected in K562 whole cell lysate Hela whole cell lysate All lanes NUP98 antibody at 1:1000. Secondary. Goat polyclonal to rabbit IgG at 1:50000 dilution. Predicted band size: 198, 188, 98, 97, 196, 187 kDa. Observed band size: 100 kDa. |
UniProt Protein Function: | NUP98: Nup98 and Nup96 play a role in the bidirectional transport across the nucleoporin complex (NPC). The repeat domain in Nup98 has a direct role in the transport. Nup98 interacts directly with Nup96. Nup96 is part of the Nup160 subcomplex in the nuclear pore which is composed of Nup160, Nup133, Nup107 and Nup96. This complex plays a role in RNA export and in tethering Nup98 and Nup153 to the nucleus. May interact with RAE1. Interacts with vesicular stomatitis virus protein M. Belongs to the nucleoporin GLFG family. 6 isoforms of the human protein are produced by alternative splicing. |
UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; Nucleoporin Chromosomal Location of Human Ortholog: 11p15.5 Cellular Component: nucleoplasm; kinetochore; nuclear membrane; nuclear pore; nuclear envelope; cytosol; nuclear inclusion body Molecular Function:protein binding; structural constituent of nuclear pore; transporter activity; peptide binding Biological Process: nuclear pore complex assembly; mRNA transport; viral reproduction; protein import into nucleus, docking; cytokine and chemokine mediated signaling pathway; mitotic nuclear envelope disassembly; nucleocytoplasmic transport; pathogenesis; viral infectious cycle; glucose transport; nuclear pore organization and biogenesis; hexose transport; carbohydrate metabolic process; viral transcription; gene expression; mitotic cell cycle; DNA replication; transmembrane transport |
NCBI Summary: | Signal-mediated nuclear import and export proceed through the nuclear pore complex (NPC), which is comprised of approximately 50 unique proteins collectively known as nucleoporins. The 98 kDa nucleoporin is generated through a biogenesis pathway that involves synthesis and proteolytic cleavage of a 186 kDa precursor protein. This cleavage results in the 98 kDa nucleoporin as well as a 96 kDa nucleoporin, both of which are localized to the nucleoplasmic side of the NPC. Rat studies show that the 98 kDa nucleoporin functions as one of several docking site nucleoporins of transport substrates. The human gene has been shown to fuse to several genes following chromosome translocations in acute myelogenous leukemia (AML) and T-cell acute lymphocytic leukemia (T-ALL). This gene is one of several genes located in the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian, and breast cancer. Alternative splicing of this gene results in several transcript variants; however, not all variants have been fully described. [provided by RefSeq, May 2010] |
UniProt Code: | P52948 |
NCBI GenInfo Identifier: | 308153660 |
NCBI Gene ID: | 4928 |
NCBI Accession: | P52948.4 |
UniProt Secondary Accession: | P52948,Q8IUT2, Q8WYB0, Q96E54, Q9H3Q4, Q9NT02, Q9UF57 Q9UHX0, Q9Y6J4, Q9Y6J5, |
UniProt Related Accession: | P52948 |
Molecular Weight: | 1817 |
NCBI Full Name: | Nuclear pore complex protein Nup98-Nup96 |
NCBI Synonym Full Names: | nucleoporin 98kDa |
NCBI Official Symbol: | NUP98Â Â |
NCBI Official Synonym Symbols: | ADIR2; NUP96; NUP196Â Â |
NCBI Protein Information: | nuclear pore complex protein Nup98-Nup96; nucleoporin 98kD; GLFG-repeat containing nucleoporin |
UniProt Protein Name: | Nuclear pore complex protein Nup98-Nup96 |
UniProt Synonym Protein Names: | 98 kDa nucleoporin; Nucleoporin Nup98; Nup98 |
UniProt Gene Name: | NUP98Â Â |
UniProt Entry Name: | NUP98_HUMAN |