Anti-NPC1L1 Antibody (RACO0556)
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주문- SKU:
- RACO0556
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- IHC
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Product Name: | NPC1L1 Antibody |
Product SKU: | RACO0556 |
Size: | 50ul |
Host Species: | Homo sapiens (Human) |
Tested Applications: | ELISA, IHC |
Recommended Dilutions: | IHC:1:50-1:200 |
Species Reactivity: | Human |
Immunogen: | A synthesized peptide derived from human NPC1L1 |
Form: | Liquid |
Storage Buffer: | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Purification Method: | Affinity-chromatography |
Clonality: | Monoclonal |
Isotype: | Rabbit IgG |
Conjugate: | Non-conjugated |
Background: | Plays a major role in cholesterol homeostasis. Is critical for the uptake of cholesterol across the plasma membrane of the intestinal enterocyte. Is the direct molecular target of ezetimibe, a drug that inhibits cholesterol absorption. Lack of activity leads to multiple lipid transport defects. The protein may have a function in the transport of multiple lipids and their homeostasis, and may play a critical role in regulating lipid metabolism. Acts as a negative regulator of NPC2 and down-regulates its expression and secretion by inhibiting its maturation and accelerating its degradation. |
Synonyms: | NPC1-like intracellular cholesterol transporter 1 (Niemann-Pick C1-like protein 1), NPC1L1 |
IHC image of RACO0556 diluted at 1:100 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. |
UniProt Protein Function: | NPC1L1: Play a major role in cholesterol homeostasis. Is critical for the uptake of cholesterol across the plasma membrane of the intestinal enterocyte. Is the direct molecular target of ezetimibe, a drug that inhibits cholesterol absorbtion. Lack of activity leads to multiple lipid transport defects. The protein may have a function in the transport of multiple lipids and their homeostasis, and may play a critical role in regulating lipid metabolism. Interacts with RAB11A, MYO5B and RAB11FIP2. Interaction with RAB11A, MYO5B and RAB11FIP2 is required for proper transport to the plasma membrane upon cholesterol depletion. Expression is decreased in Caco-2 cells upon PPARD activation. Widely expressed. Expressed in liver. Also expressed in small intestine, pancreas, kidney, lung, pancreas, spleen, heart, gall bladder, brain, testis, stomach and muscle. Belongs to the patched family. 3 isoforms of the human protein are produced by alternative splicing. |
UniProt Protein Details: | Protein type:Membrane protein, integral; Membrane protein, multi-pass Chromosomal Location of Human Ortholog: 7p13 Cellular Component: apical plasma membrane; brush border membrane; cytoplasmic vesicle membrane; integral component of membrane; plasma membrane Molecular Function:drug binding; myosin V binding; protein binding; Rab GTPase binding Biological Process: cholesterol biosynthetic process; cholesterol transport; intestinal cholesterol absorption; lipoprotein metabolic process; response to drug |
NCBI Summary: | The protein encoded by this gene is a multi-pass membrane protein. It contains a conserved N-terminal Niemann-Pick C1 (NPC1) domain and a putative sterol-sensing domain (SSD) which includes a YQRL motif functioning as a plasma membrane to trans-Golgi network transport signal in other proteins. This protein takes up free cholesterol into cells through vesicular endocytosis and plays a critical role in the absorption of intestinal cholesterol. It also has the ability to transport alpha-tocopherol (vitamin E). The drug ezetimibe targets this protein and inhibits the absorption of intestinal cholesterol and alpha-tocopherol. In addition, this protein may play a critical role in regulating lipid metabolism. Polymorphic variations in this gene are associated with plasma total cholesterol and low-density lipoprotein cholesterol (LDL-C) levels and coronary heart disease (CHD) risk. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009] |
UniProt Code: | Q9UHC9 |
NCBI GenInfo Identifier: | 156231353 |
NCBI Gene ID: | 29881 |
NCBI Accession: | NP_001095118.1 |
UniProt Related Accession: | Q9UHC9 |
Molecular Weight: | 149kDa |
NCBI Full Name: | NPC1-like intracellular cholesterol transporter 1 isoform 2 |
NCBI Synonym Full Names: | NPC1 like intracellular cholesterol transporter 1 |
NCBI Official Symbol: | NPC1L1Â Â |
NCBI Official Synonym Symbols: | LDLCQ7; NPC11L1; SLC65A2Â Â |
NCBI Protein Information: | NPC1-like intracellular cholesterol transporter 1 |
UniProt Protein Name: | Niemann-Pick C1-like protein 1 |
Protein Family: | Niemann-Pick C1-like protein |
UniProt Gene Name: | NPC1L1Â Â |