Anti-MYBBP1A Antibody (RACO0547)
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주문- SKU:
- RACO0547
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- IF
- Application:
- ELISA
- Application:
- IHC
- Conjugation:
- Unconjugated
Description
Product Name: | MYBBP1A Antibody |
Product SKU: | RACO0547 |
Size: | 50ul |
Host Species: | Homo sapiens (Human) |
Tested Applications: | ELISA, IHC, IF |
Recommended Dilutions: | IHC:1:50-1:200, IF:1:20-1:200 |
Species Reactivity: | Human |
Immunogen: | A synthesized peptide derived from human MYBBP1A |
Form: | Liquid |
Storage Buffer: | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Purification Method: | Affinity-chromatography |
Clonality: | Monoclonal |
Isotype: | Rabbit IgG |
Conjugate: | Non-conjugated |
Background: | May activate or repress transcription via interactions with sequence specific DNA-binding proteins. Repression may be mediated at least in part by histone deacetylase activity (HDAC activity). Acts as a corepressor and in concert with CRY1, represses the transcription of the core circadian clock component PER2. Preferentially binds to dimethylated histone H3 'Lys-9' (H3K9me2) on the PER2 promoter. |
Synonyms: | Myb-binding protein 1A, MYBBP1A, P160 |
Immunofluorescence staining of HepG2 Cells with RACO0547 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L). | |
IHC image of RACO0547 diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. |
UniProt Protein Function: | MYBBP1A: may activate or repress transcription via interactions with sequence specific DNA-binding proteins. Repression may be mediated at least in part by histone deacetylase activity (HDAC activity). Binds to and represses JUN and MYB via the leucine zipper regions present in these proteins. Also binds to and represses PGC-1 (PPAR gamma coactivator-1): this interaction is abrogated when PGC-1 is phosphorylated by ERK1. Binds to and stimulates transcription by AhR. Binds to KPNA2. Shuttles between the nucleus and cytoplasm. Nuclear import may be mediated by KPNA2, while export appears to depend partially on exportin 1. Predominantly nucleolar. Two alternatively spliced isoforms have been described. |
UniProt Protein Details: | Protein type:Nucleolus; DNA replication; DNA-binding; RNA-binding Chromosomal Location of Human Ortholog: 17p13.3 Cellular Component: NLS-dependent protein nuclear import complex; intracellular membrane-bound organelle; membrane; cytoplasm; nucleolus; nucleus Molecular Function:protein binding; sequence-specific DNA binding; DNA-directed DNA polymerase activity; transcription corepressor activity; transcription factor binding Biological Process: osteoblast differentiation; cellular response to glucose starvation; regulation of transcription, DNA-dependent; transcription, DNA-dependent; nucleocytoplasmic transport; circadian regulation of gene expression; negative regulation of transcription, DNA-dependent |
NCBI Summary: | This gene encodes a nucleolar transcriptional regulator that was first identified by its ability to bind specifically to the Myb proto-oncogene protein. The encoded protein is thought to play a role in many cellular processes including response to nucleolar stress, tumor suppression and synthesis of ribosomal DNA. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013] |
UniProt Code: | Q9BQG0 |
NCBI GenInfo Identifier: | 71153825 |
NCBI Gene ID: | 10514 |
NCBI Accession: | Q9BQG0.2 |
UniProt Secondary Accession: | Q9BQG0,Q86VM3, Q9BW49, Q9P0V5, Q9UF99, |
UniProt Related Accession: | Q9BQG0 |
Molecular Weight: | 1328 |
NCBI Full Name: | Myb-binding protein 1A |
NCBI Synonym Full Names: | MYB binding protein (P160) 1a |
NCBI Official Symbol: | MYBBP1AÂ Â |
NCBI Official Synonym Symbols: | P160; PAP2Â Â |
NCBI Protein Information: | myb-binding protein 1A; p53-activated protein-2 |
UniProt Protein Name: | Myb-binding protein 1A |
Protein Family: | Myb-binding protein |
UniProt Gene Name: | MYBBP1AÂ Â |
UniProt Entry Name: | MBB1A_HUMAN |