Anti-MAP2K1 Antibody (RACO0276)
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주문- SKU:
- RACO0276
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Reactivity:
- Rat
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- IP
- Application:
- WB
- Application:
- ELISA
- Application:
- IHC
- Conjugation:
- Unconjugated
Description
Product Name: | MAP2K1 Antibody |
Product SKU: | RACO0276 |
Size: | 50ul |
Host Species: | Homo sapiens (Human) |
Tested Applications: | ELISA, WB, IHC, IP |
Recommended Dilutions: | WB:1:500-1:5000, IHC:1:50-1:200, IP:1:200-1:1000 |
Species Reactivity: | Human, Rat |
Immunogen: | A synthesized peptide derived from human MEK1 |
Form: | Liquid |
Storage Buffer: | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Purification Method: | Affinity-chromatography |
Clonality: | Monoclonal |
Isotype: | Rabbit IgG |
Conjugate: | Non-conjugated |
Background: | Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Binding of extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation. RAF1 then further activates the dual-specificity protein kinases MAP2K1/MEK1 and MAP2K2/MEK2. Both MAP2K1/MEK1 and MAP2K2/MEK2 function specifically in the MAPK/ERK cascade, and catalyze the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in the extracellular signal-regulated kinases MAPK3/ERK1 and MAPK1/ERK2, leading to their activation and further transduction of the signal within the MAPK/ERK cascade. Depending on the cellular context, this pathway mediates diverse biological functions such as cell growth, adhesion, survival and differentiation, predominantly through the regulation of transcription, metabolism and cytoskeletal rearrangements. One target of the MAPK/ERK cascade is peroxisome proliferator-activated receptor gamma (PPARG), a nuclear receptor that promotes differentiation and apoptosis. MAP2K1/MEK1 has been shown to export PPARG from the nucleus. The MAPK/ERK cascade is also involved in the regulation of endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC), as well as in the fragmentation of the Golgi apparatus during mitosis. |
Synonyms: | Dual specificity mitogen-activated protein kinase kinase 1 (MAP kinase kinase 1) (MAPKK 1) (MKK1) (EC 2.7.12.2) (ERK activator kinase 1) (MAPK/ERK kinase 1) (MEK 1), MAP2K1, MEK1 PRKMK1 |
IHC image of RACO0276 diluted at 1:100 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. | |
Immunoprecipitating MAP2K1 in Hela whole cell lysate Lane 1: Rabbit control IgG instead of RACO0276 in Hela whole cell lysate For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: RACO0276(2µg)+ Hela whole cell lysate (500µg). Lane 3: Hela whole cell lysate (10µg). | |
Western Blot. Positive WB detected in Hela whole cell lysate 293 whole cell lysate MCF-7 whole cell lysate 293T whole cell lysate A549 whole cell lysate U251 whole cell lysate Rat brain tissue. All lanes MAP2K1 antibody at 1:2000. Secondary. Goat polyclonal to rabbit IgG at 1:50000 dilution. Predicted band size: 44, 41 kDa. Observed band size: 44 kDa. |
UniProt Protein Function: | MEK1: a dual-specificity protein kinase of the STE7 kinase family. Phosphorylated and activated by Raf, Mos and Cot kinases. Phosphorylates a Thr and a Tyr residue in a Thr-Glu-Tyr sequence located in the activation loop of ERK1 and ERK2. An essential component of MAP kinase signal transduction pathways involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. |
UniProt Protein Details: | Protein type:EC 2.7.12.2; Protein kinase, dual-specificity (non-receptor); Kinase, protein; Protein kinase, STE; STE group; STE7 family Chromosomal Location of Human Ortholog: 15q22.1-q22.33 Cellular Component: dendrite cytoplasm; Golgi apparatus; microtubule; focal adhesion; mitochondrion; endoplasmic reticulum; early endosome; perikaryon; cell cortex; cytosol; axon; perinuclear region of cytoplasm; late endosome; cytoplasm; plasma membrane; microtubule organizing center; nucleus Molecular Function:MAP kinase kinase activity; protein serine/threonine kinase activity; protein serine/threonine kinase activator activity; protein binding; Ras GTPase binding; receptor signaling protein tyrosine phosphatase activity; protein-tyrosine kinase activity; protein serine/threonine/tyrosine kinase activity; mitogen-activated protein kinase kinase kinase binding; ATP binding; protein kinase activity Biological Process: axon guidance; peptidyl-tyrosine phosphorylation; activation of MAPKK activity; nerve growth factor receptor signaling pathway; activation of MAPK activity; protein heterooligomerization; negative regulation of homotypic cell-cell adhesion; response to glucocorticoid stimulus; stress-activated MAPK cascade; cell motility involved in cell locomotion; pathogenesis; toll-like receptor 3 signaling pathway; signal transduction; chemotaxis; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; neuron differentiation; negative regulation of cell proliferation; positive regulation of RNA elongation from RNA polymerase II promoter; small GTPase mediated signal transduction; vesicle transport along microtubule; response to axon injury; cell cycle arrest; toll-like receptor 4 signaling pathway; Golgi inheritance; epidermal growth factor receptor signaling pathway; mitosis; fibroblast growth factor receptor signaling pathway; MyD88-independent toll-like receptor signaling pathway; MAPKKK cascade; melanosome transport; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; keratinocyte differentiation; cell proliferation; regulation of stress-activated MAPK cascade; regulation of vascular smooth muscle contraction; Ras protein signal transduction; insulin receptor signaling pathway; toll-like receptor signaling pathway; innate immune response; toll-like receptor 9 signaling pathway; response to oxidative stress; positive regulation of Ras protein signal transduction; cell motility; positive regulation of cell differentiation; vascular endothelial growth factor receptor signaling pathway; positive regulation of cell migration Disease: Noonan Syndrome 1; Cardiofaciocutaneous Syndrome 3 |
NCBI Summary: | The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. [provided by RefSeq, Jul 2008] |
UniProt Code: | Q02750 |
NCBI GenInfo Identifier: | 400274 |
NCBI Gene ID: | 5604 |
NCBI Accession: | Q02750.2 |
UniProt Related Accession: | Q02750 |
Molecular Weight: | 393 |
NCBI Full Name: | Dual specificity mitogen-activated protein kinase kinase 1 |
NCBI Synonym Full Names: | mitogen-activated protein kinase kinase 1 |
NCBI Official Symbol: | MAP2K1Â Â |
NCBI Official Synonym Symbols: | CFC3; MEK1; MKK1; MAPKK1; PRKMK1Â Â |
NCBI Protein Information: | dual specificity mitogen-activated protein kinase kinase 1; MEK 1; MAPKK 1; MAPK/ERK kinase 1; ERK activator kinase 1; protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1) |
UniProt Protein Name: | Dual specificity mitogen-activated protein kinase kinase 1 |
UniProt Synonym Protein Names: | ERK activator kinase 1; MAPK/ERK kinase 1; MEK 1 |
UniProt Gene Name: | MAP2K1Â Â |
UniProt Entry Name: | MP2K1_HUMAN |