Anti-LDLR Antibody (RACO0220)
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주문- SKU:
- RACO0220
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- IF
- Application:
- ELISA
- Application:
- IHC
- Application:
- FC
- Conjugation:
- Unconjugated
Description
Product Name: | LDLR Antibody |
Product SKU: | RACO0220 |
Size: | 50ul |
Host Species: | Homo sapiens (Human) |
Tested Applications: | ELISA, IHC, IF, FC |
Recommended Dilutions: | IHC:1:50-1:200, IF:1:20-1:200, FC:1:20-1:200 |
Species Reactivity: | Human |
Immunogen: | A synthesized peptide derived from human LDL Receptor |
Form: | Liquid |
Storage Buffer: | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Purification Method: | Affinity-chromatography |
Clonality: | Monoclonal |
Isotype: | Rabbit IgG |
Conjugate: | Non-conjugated |
Background: | Binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. |
Synonyms: | Low-density lipoprotein receptor (LDL receptor), LDLR |
IHC image of RACO0220 diluted at 1:100 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. | |
Immunofluorescence staining of Hela Cells with RACO0220 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L). | |
IHC image of RACO0220 diluted at 1:100 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. |
UniProt Protein Function: | LDLR: Binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. In case of HIV-1 infection, functions as a receptor for extracellular Tat in neurons, mediating its internalization in uninfected cells. Defects in LDLR are the cause of familial hypercholesterolemia (FH); a common autosomal semi- dominant disease that affects about 1 in 500 individuals. The receptor defect impairs the catabolism of LDL, and the resultant elevation in plasma LDL-cholesterol promotes deposition of cholesterol in the skin (xanthelasma), tendons (xanthomas), and coronary arteries (atherosclerosis). Belongs to the LDLR family. 4 isoforms of the human protein are produced by alternative splicing. |
UniProt Protein Details: | Protein type:Receptor, misc.; Membrane protein, integral; Cell surface Chromosomal Location of Human Ortholog: 19p13.2 Cellular Component: apical part of cell; basolateral plasma membrane; cell surface; coated pit; early endosome; endosome membrane; external side of plasma membrane; Golgi apparatus; integral to plasma membrane; late endosome; lysosome; membrane; plasma membrane; receptor complex Molecular Function:clathrin heavy chain binding; glycoprotein binding; identical protein binding; low-density lipoprotein receptor activity; protease binding; protein binding; receptor activity; very-low-density lipoprotein receptor activity Biological Process: cholesterol absorption; cholesterol homeostasis; cholesterol transport; endocytosis; lipid metabolic process; phospholipid transport; receptor-mediated endocytosis Disease: Hypercholesterolemia, Familial |
NCBI Summary: | The low density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. Low density lipoprotein (LDL) is normally bound at the cell membrane and taken into the cell ending up in lysosomes where the protein is degraded and the cholesterol is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. Mutations in this gene cause the autosomal dominant disorder, familial hypercholesterolemia. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Sep 2010] |
UniProt Code: | P01130 |
NCBI GenInfo Identifier: | 126073 |
NCBI Gene ID: | 3949 |
NCBI Accession: | P01130.1 |
UniProt Secondary Accession: | P01130,Q53ZD9, Q59FQ1, Q9UDH7, B4DII3, B4DJZ8, B4DR00 B4DTQ3, C0JYY8, H0YLU8, H0YNT7, |
UniProt Related Accession: | P01130 |
Molecular Weight: | 82,255 Da |
NCBI Full Name: | Low-density lipoprotein receptor |
NCBI Synonym Full Names: | low density lipoprotein receptor |
NCBI Official Symbol: | LDLRÂ Â |
NCBI Official Synonym Symbols: | FH; FHC; LDLCQ2Â Â |
NCBI Protein Information: | low-density lipoprotein receptor |
UniProt Protein Name: | Low-density lipoprotein receptor |
Protein Family: | LDLR chaperone |
UniProt Gene Name: | LDLRÂ Â |
UniProt Entry Name: | LDLR_HUMAN |