Anti-KLF4 Antibody (RACO0307)
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주문- SKU:
- RACO0307
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- IP
- Application:
- WB
- Application:
- IF
- Application:
- ELISA
- Application:
- IHC
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Product Name: | KLF4 Antibody |
Product SKU: | RACO0307 |
Size: | 50ul |
Host Species: | Homo sapiens (Human) |
Tested Applications: | ELISA, WB, IHC, IF, IP |
Recommended Dilutions: | WB:1:500-1:5000, IHC:1:50-1:200, IF:1:20-1:200, IP:1:200-1:1000 |
Species Reactivity: | Human |
Immunogen: | A synthesized peptide derived from human KLF4 |
Form: | Liquid |
Storage Buffer: | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Purification Method: | Affinity-chromatography |
Clonality: | Monoclonal |
Isotype: | Rabbit IgG |
Conjugate: | Non-conjugated |
Background: | Transcription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription. |
Synonyms: | Krueppel-like factor 4 (Epithelial zinc finger protein EZF) (Gut-enriched krueppel-like factor), KLF4, EZF GKLF |
IHC image of RACO0307 diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. | |
IHC image of RACO0307 diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. | |
Western Blot. Positive WB detected in Hela whole cell lysate 293T whole cell lysate PC-3 whole cell lysate MCF-7 whole cell lysate A549 whole cell lysate U87 whole cell lysate Brain tissue. All lanes KLF4 antibody at 1:1500. Secondary. Goat polyclonal to rabbit IgG at 1:50000 dilution. Predicted band size: 55, 52, 46, 13, 7 kDa. Observed band size: 55 kDa. |
UniProt Code: | O43474 |
NCBI GenInfo Identifier: | 194248077 |
NCBI Gene ID: | 9314 |
NCBI Accession: | NP_004226.3 |
UniProt Secondary Accession: | O43474,P78338, Q5T3J8, Q5T3J9, Q8N717, Q9UNP3, B2R8S4 B3KT79, L0R3I6, L0R4N5, |
UniProt Related Accession: | O43474 |
Molecular Weight: | 51kDa |
NCBI Full Name: | Krueppel-like factor 4 |
NCBI Synonym Full Names: | Kruppel-like factor 4 (gut) |
NCBI Official Symbol: | KLF4Â Â |
NCBI Official Synonym Symbols: | EZF; GKLFÂ Â |
NCBI Protein Information: | Krueppel-like factor 4; gut-enriched krueppel-like factor; epithelial zinc finger protein EZF; endothelial Kruppel-like zinc finger protein |
UniProt Protein Name: | Krueppel-like factor 4 |
UniProt Synonym Protein Names: | Epithelial zinc finger protein EZF; Gut-enriched krueppel-like factor |
Protein Family: | Krueppel-like factor |
UniProt Gene Name: | KLF4Â Â |
UniProt Entry Name: | KLF4_HUMAN |