Anti-JUNB Antibody (RACO0490)
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주문- SKU:
- RACO0490
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- IF
- Application:
- ELISA
- Application:
- IHC
- Conjugation:
- Unconjugated
Description
Product Name: | JUNB Antibody |
Product SKU: | RACO0490 |
Size: | 50ul |
Host Species: | Homo sapiens (Human) |
Tested Applications: | ELISA, IHC, IF |
Recommended Dilutions: | IHC:1:50-1:200, IF:1:20-1:200 |
Species Reactivity: | Human |
Immunogen: | A synthesized peptide derived from human JunB |
Form: | Liquid |
Storage Buffer: | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Purification Method: | Affinity-chromatography |
Clonality: | Monoclonal |
Isotype: | Rabbit IgG |
Conjugate: | Non-conjugated |
Background: | Transcription factor involved in regulating gene activity following the primary growth factor response. Binds to the DNA sequence 5'-TGA[CG]TCA-3'. |
Synonyms: | Transcription factor jun-B, JUNB |
Immunofluorescence staining of MCF7 Cells with RACO0490 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L). | |
IHC image of RACO0490 diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. |
UniProt Protein Function: | JunB: Transcription factor involved in regulating gene activity following the primary growth factor response. Binds to the DNA sequence 5'-TGA[CG]TCA-3'. Binds DNA as an homodimer or as an heterodimer with another member of the Jun/Fos family. Interacts with ITCH (via its WW domains). By growth factors. Belongs to the bZIP family. Jun subfamily. |
UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; Transcription factor; Oncoprotein Chromosomal Location of Human Ortholog: 19p13.2 Cellular Component: chromatin; nuclear chromatin; nucleoplasm; transcription factor complex Molecular Function:DNA binding; protein binding; transcription coactivator activity; transcription corepressor activity; transcription factor binding Biological Process: cellular response to hormone stimulus; negative regulation of transcription from RNA polymerase II promoter; positive regulation of cell differentiation; positive regulation of transcription from RNA polymerase II promoter; regulation of cell cycle; regulation of cell proliferation; regulation of transcription from RNA polymerase II promoter; response to cAMP; response to cytokine stimulus; response to drug; response to lipopolysaccharide; response to mechanical stimulus; response to radiation |
UniProt Code: | P17275 |
NCBI GenInfo Identifier: | 135304 |
NCBI Gene ID: | 3726 |
NCBI Accession: | P17275.1 |
UniProt Secondary Accession: | P17275,Q96GH3, |
UniProt Related Accession: | P17275 |
Molecular Weight: | 35,879 Da |
NCBI Full Name: | Transcription factor jun-B |
NCBI Synonym Full Names: | JunB proto-oncogene, AP-1 transcription factor subunit |
NCBI Official Symbol: | JUNBÂ Â |
NCBI Official Synonym Symbols: | AP-1Â Â |
NCBI Protein Information: | transcription factor jun-B |
UniProt Protein Name: | Transcription factor jun-B |
Protein Family: | Transcription factor |
UniProt Gene Name: | JUNBÂ Â |
UniProt Entry Name: | JUNB_HUMAN |