Anti-IRAK4 Antibody (RACO0370)
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주문- SKU:
- RACO0370
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- WB
- Application:
- ELISA
- Application:
- FC
- Conjugation:
- Unconjugated
Description
Product Name: | IRAK4 Antibody |
Product SKU: | RACO0370 |
Size: | 50ul |
Host Species: | Homo sapiens (Human) |
Tested Applications: | ELISA, WB, FC |
Recommended Dilutions: | WB:1:500-1:5000, FC:1:20-1:200 |
Species Reactivity: | Human |
Immunogen: | A synthesized peptide derived from human IRAK4 |
Form: | Liquid |
Storage Buffer: | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Purification Method: | Affinity-chromatography |
Clonality: | Monoclonal |
Isotype: | Rabbit IgG |
Conjugate: | Non-conjugated |
Background: | Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways (PubMed:17878374). Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation to form the Myddosome together with IRAK2. Phosphorylates initially IRAK1, thus stimulating the kinase activity and intensive autophosphorylation of IRAK1. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates NCF1 and regulates NADPH oxidase activation after LPS stimulation suggesting a similar mechanism during microbial infections. |
Synonyms: | Interleukin-1 receptor-associated kinase 4 (IRAK-4) (EC 2.7.11.1) (Renal carcinoma antigen NY-REN-64), IRAK4 |
Overlay histogram showing Jurkat cells stained with RACO0370 (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1µg) 1*106cells) for 1 h at 4°C.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4°C. Control antibody (green line) was Rabbit IgG (1µg) 1*106cells) used under the same conditions. Acquisition of >10,000 events was performed. | |
Western Blot. Positive WB detected in THP-1 whole cell lysate U251 whole cell lysate All lanes IRAK4 antibody at 1:2000. Secondary. Goat polyclonal to rabbit IgG at 1:50000 dilution. Predicted band size: 52, 38 kDa. Observed band size: 55 kDa. |
UniProt Protein Function: | IRAK4: a TKL kinase of the IRAK family. The interleukin-1 receptor-associated kinases are important mediators in the signal transduction of Toll-like receptor and IL1R family members, collectively referred to as TIRs. |
UniProt Protein Details: | Protein type:EC 2.7.11.1; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, TKL; TKL group; IRAK family Chromosomal Location of Human Ortholog: 12q12 Cellular Component: extracellular space; cytoplasm; plasma membrane; endosome membrane; cytosol; nucleus Molecular Function:protein serine/threonine kinase activity; protein binding; interleukin-1 receptor binding; magnesium ion binding; ATP binding; protein kinase activity Biological Process: positive regulation of I-kappaB kinase/NF-kappaB cascade; cytokine and chemokine mediated signaling pathway; positive regulation of smooth muscle cell proliferation; cytokine production; protein amino acid phosphorylation; toll-like receptor 10 signaling pathway; toll-like receptor 2 signaling pathway; toll-like receptor 5 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; toll-like receptor signaling pathway; JNK cascade; innate immune response; toll-like receptor 9 signaling pathway; toll-like receptor 4 signaling pathway Disease: Invasive Pneumococcal Disease, Recurrent Isolated, 1; Irak4 Deficiency |
NCBI Summary: | This gene encodes a kinase that activates NF-kappaB in both the Toll-like receptor (TLR) and T-cell receptor (TCR) signaling pathways. The protein is essential for most innate immune responses. Mutations in this gene result in IRAK4 deficiency and recurrent invasive pneumococcal disease. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011] |
UniProt Code: | Q9NWZ3 |
NCBI GenInfo Identifier: | 50401181 |
NCBI Gene ID: | 51135 |
NCBI Accession: | Q9NWZ3.1 |
UniProt Secondary Accession: | Q9NWZ3,Q69FE1, Q8TDF7, Q9Y589, |
UniProt Related Accession: | Q9NWZ3 |
Molecular Weight: | 37,674 Da |
NCBI Full Name: | Interleukin-1 receptor-associated kinase 4 |
NCBI Synonym Full Names: | interleukin-1 receptor-associated kinase 4 |
NCBI Official Symbol: | IRAK4Â Â |
NCBI Official Synonym Symbols: | IPD1; REN64; IRAK-4; NY-REN-64Â Â |
NCBI Protein Information: | interleukin-1 receptor-associated kinase 4; renal carcinoma antigen NY-REN-64 |
UniProt Protein Name: | Interleukin-1 receptor-associated kinase 4 |
UniProt Synonym Protein Names: | Renal carcinoma antigen NY-REN-64 |
Protein Family: | Interleukin-1 receptor-associated kinase |
UniProt Gene Name: | IRAK4Â Â |
UniProt Entry Name: | IRAK4_HUMAN |