Anti-HNRNPK Antibody (RACO0157)
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주문- SKU:
- RACO0157
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- IP
- Application:
- WB
- Application:
- IF
- Application:
- ELISA
- Application:
- IHC
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Product Name: | HNRNPK Antibody |
Product SKU: | RACO0157 |
Size: | 50ul |
Host Species: | Human |
Tested Applications: | ELISA, WB, IHC, IF, IP |
Recommended Dilutions: | WB:1:500-1:5000, IHC:1:50-1:200, IF:1:20-1:200, IP:1:200-1:1000 |
Species Reactivity: | Human |
Immunogen: | A synthesized peptide derived from human HNRNPK |
Form: | Liquid |
Storage Buffer: | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Purification Method: | Affinity-chromatography |
Clonality: | Monoclonal |
Isotype: | Rabbit IgG |
Conjugate: | Non-conjugated |
Background: | One of the major pre-mRNA-binding proteins. Binds tenaciously to poly(C) sequences. Likely to play a role in the nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich sequences. Can also bind poly(C) single-stranded DNA. Plays an important role in p53/TP53 response to DNA damage, acting at the level of both transcription activation and repression. When sumoylated, acts as a transcriptional coactivator of p53/TP53, playing a role in p21/CDKN1A and 14-3-3 sigma/SFN induction (By similarity). As far as transcription repression is concerned, acts by interacting with long intergenic RNA p21 (lincRNA-p21), a non-coding RNA induced by p53/TP53. This interaction is necessary for the induction of apoptosis, but not cell cycle arrest. |
Synonyms: | Heterogeneous nuclear ribonucleoprotein K, hnRNP K, Transformation up-regulated nuclear protein, TUNP, HNRNPK, HNRPK |
IHC image of RACO0157 diluted at 1:130.5 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. | |
IHC image of RACO0157 diluted at 1:130.5 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. | |
Western Blot. Positive WB detected in Hela whole cell lysate MCF-7 whole cell lysate HepG2 whole cell lysate 293T whole cell lysate 293 whole cell lysate U87 whole cell lysate SH-SY5Y whole cell lysate All lanes HNRNPK antibody at 1 (3µg) ml. Secondary. Goat polyclonal to rabbit IgG at 1:50000 dilution. Predicted band size: 51, 52, 49 KDa. Observed band size: 60 KDa. |
UniProt Protein Function: | hnRNP K: a heterogeneous nuclear ribonucleoprotein (hnRNP). One of the major pre-mRNA-binding proteins. Binds tenaciously to poly(C) sequences. Likely to play a role in the nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich sequences. Can also bind poly(C) single-stranded DNA. Two alternatively spliced isoforms have been described. |
UniProt Protein Details: | Protein type:RNA splicing; RNA-binding; Spliceosome Chromosomal Location of Human Ortholog: 9q21.32 Cellular Component: cell-cell adherens junction; extracellular matrix; focal adhesion; membrane; nuclear chromatin; nucleoplasm; nucleus Molecular Function:protein binding; RNA binding; single-stranded DNA binding Biological Process: gene expression; negative regulation of apoptosis; nuclear mRNA splicing, via spliceosome; positive regulation of low-density lipoprotein receptor biosynthetic process; positive regulation of receptor-mediated endocytosis; positive regulation of transcription from RNA polymerase II promoter; protein sumoylation; RNA processing; signal transduction Disease: Au-kline Syndrome |
NCBI Summary: | This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene is located in the nucleoplasm and has three repeats of KH domains that binds to RNAs. It is distinct among other hnRNP proteins in its binding preference; it binds tenaciously to poly(C). This protein is also thought to have a role during cell cycle progession. Several alternatively spliced transcript variants have been described for this gene, however, not all of them are fully characterized. [provided by RefSeq, Jul 2008] |
UniProt Code: | P61978 |
NCBI GenInfo Identifier: | 48429103 |
NCBI Gene ID: | 3190 |
NCBI Accession: | P61978.1 |
UniProt Secondary Accession: | P61978,Q07244, Q15671, Q59F98, Q5T6W4, Q60577, Q922Y7 Q96J62, |
UniProt Related Accession: | P61978 |
Molecular Weight: | 48,562 Da |
NCBI Full Name: | Heterogeneous nuclear ribonucleoprotein K |
NCBI Synonym Full Names: | heterogeneous nuclear ribonucleoprotein K |
NCBI Official Symbol: | HNRNPKÂ Â |
NCBI Official Synonym Symbols: | AUKS; CSBP; TUNP; HNRPKÂ Â |
NCBI Protein Information: | heterogeneous nuclear ribonucleoprotein K |
UniProt Protein Name: | Heterogeneous nuclear ribonucleoprotein K |
UniProt Synonym Protein Names: | Transformation up-regulated nuclear protein; TUNP |
Protein Family: | Heterogeneous nuclear ribonucleoprotein |
UniProt Gene Name: | HNRNPKÂ Â |