Anti-HDAC9 Antibody (RACO0135)
제품 코드를 사용하여 Assay Genie 메인 사이트를 통해 주문합니다.
주문- SKU:
- RACO0135
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- WB
- Application:
- IF
- Application:
- ELISA
- Application:
- IHC
- Conjugation:
- Unconjugated
Description
Product Name: | HDAC9 Antibody |
Product SKU: | RACO0135 |
Size: | 50ul |
Host Species: | Human |
Tested Applications: | ELISA, WB, IHC, IF |
Recommended Dilutions: | WB:1:500-1:5000, IHC:1:50-1:200, IF:1:20-1:200 |
Species Reactivity: | Human |
Immunogen: | A synthesized peptide derived from human HDAC9 |
Form: | Liquid |
Storage Buffer: | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Purification Method: | Affinity-chromatography |
Clonality: | Monoclonal |
Isotype: | Rabbit IgG |
Conjugate: | Non-conjugated |
Background: | Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Represses MEF2-dependent transcription. |
Synonyms: | Histone deacetylase 9, HD9, Histone deacetylase 7B, HD7, HD7b, Histone deacetylase-related protein, MEF2-interacting transcription repressor MITR, HDAC9, HDAC7, HDAC7B, HDRP, KIAA0744, MITR |
IHC image of RACO0135 diluted at 1:154 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. | |
IHC image of RACO0135 diluted at 1:154 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. | |
Western Blot. Positive WB detected in Hela whole cell lysate MCF-7 whole cell lysate 293T whole cell lysate K562 whole cell lysate All lanes HDAC9 antibody at 1.54µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1:50000 dilution. Predicted band size: 112, 102, 66, 98, 118, 113, 61, 63, 58 KDa. Observed band size: 160 KDa. |
UniProt Protein Function: | HDAC9: a transcriptional regulator of the histone deacetylase family, subfamily 2. Deacetylates lysine residues on the N-terminal part of the core histones H2A, H2B, H3 AND H4. Plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. |
UniProt Protein Details: | Protein type:Deacetylase; EC 3.5.1.98; Transcription, coactivator/corepressor Chromosomal Location of Human Ortholog: 7p21.1 Cellular Component: nucleoplasm; histone methyltransferase complex; transcription factor complex; cytoplasm; histone deacetylase complex; nucleus Molecular Function:NAD-dependent histone deacetylase activity (H3-K9 specific); protein binding; protein kinase C binding; NAD-dependent histone deacetylase activity (H3-K14 specific); metal ion binding; histone deacetylase binding; protein deacetylase activity; NAD-dependent histone deacetylase activity (H4-K16 specific); histone deacetylase activity; transcription corepressor activity; transcription factor binding Biological Process: regulation of skeletal muscle fiber development; Notch signaling pathway; B cell activation; cellular response to insulin stimulus; transcription, DNA-dependent; regulation of striated muscle cell differentiation; B cell differentiation; heart development; histone deacetylation; negative regulation of transcription from RNA polymerase II promoter; inflammatory response; negative regulation of transcription, DNA-dependent |
NCBI Summary: | Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene has sequence homology to members of the histone deacetylase family. This gene is orthologous to the Xenopus and mouse MITR genes. The MITR protein lacks the histone deacetylase catalytic domain. It represses MEF2 activity through recruitment of multicomponent corepressor complexes that include CtBP and HDACs. This encoded protein may play a role in hematopoiesis. Multiple alternatively spliced transcripts have been described for this gene but the full-length nature of some of them has not been determined. [provided by RefSeq, Jul 2008] |
UniProt Code: | Q9UKV0 |
NCBI GenInfo Identifier: | 19865267 |
NCBI Gene ID: | 9734 |
NCBI Accession: | Q9UKV0.2 |
UniProt Secondary Accession: | Q9UKV0,O94845, O95028, Q2M2R6, A7E2F3, B7Z4I4, B7Z917 B7Z928, B7Z940, C9JS87, E7EX34, F8W9E0, |
UniProt Related Accession: | Q9UKV0 |
Molecular Weight: | 1011 |
NCBI Full Name: | Histone deacetylase 9 |
NCBI Synonym Full Names: | histone deacetylase 9 |
NCBI Official Symbol: | HDAC9Â Â |
NCBI Official Synonym Symbols: | HD7; HD9; HD7b; HDAC; HDRP; MITR; HDAC7; HDAC7B; HDAC9B; HDAC9FLÂ Â |
NCBI Protein Information: | histone deacetylase 9; histone deacetylase 7B; histone deacetylase 4/5-related protein; MEF-2 interacting transcription repressor (MITR) protein |
UniProt Protein Name: | Histone deacetylase 9 |
UniProt Synonym Protein Names: | Histone deacetylase 7B; HD7; HD7b; Histone deacetylase-related protein; MEF2-interacting transcription repressor MITR |
UniProt Gene Name: | HDAC9Â Â |
UniProt Entry Name: | HDAC9_HUMAN |