Anti-GDNF Antibody (RACO0166)
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주문- SKU:
- RACO0166
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- IP
- Application:
- WB
- Application:
- ELISA
- Application:
- IHC
- Application:
- FC
- Conjugation:
- Unconjugated
Description
Product Name: | GDNF Antibody |
Product SKU: | RACO0166 |
Size: | 50ul |
Host Species: | Human |
Tested Applications: | ELISA, WB, IHC, FC, IP |
Recommended Dilutions: | WB:1:500-1:5000, IHC:1:50-1:200, IP:1:200-1:1000 |
Species Reactivity: | Human |
Immunogen: | A synthesized peptide derived from human GDNF |
Form: | Liquid |
Storage Buffer: | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Purification Method: | Affinity-chromatography |
Clonality: | Monoclonal |
Isotype: | Rabbit IgG |
Conjugate: | Non-conjugated |
Background: | Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. |
Synonyms: | Glial cell line-derived neurotrophic factor, hGDNF, Astrocyte-derived trophic factor, ATF, GDNF |
IHC image of RACO0166 diluted at 1:107.5 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. | |
IHC image of RACO0166 diluted at 1:107.5 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. | |
Western Blot. Positive WB detected in HepG2 whole cell lysate 293T whole cell lysate SH-SY5Y whole cell lysate PC3 whole cell lysate Hela whole cell lysate MCF-7 whole cell lysate U251 whole cell lysate All lanes GDNF antibody at (1µg) ml. Secondary. Goat polyclonal to rabbit IgG at 1:50000 dilution. Predicted band size: 24, 21, 26, 23, 19 KDa. Observed band size: 24 KDa. |
UniProt Protein Function: | GDNF: Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. Defects in GDNF may be a cause of Hirschsprung disease type 3 (HSCR3). In association with mutations of RET gene, defects in GDNF may be involved in Hirschsprung disease. This genetic disorder of neural crest development is characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction. Defects in GDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. Belongs to the TGF-beta family. GDNF subfamily. 5 isoforms of the human protein are produced by alternative splicing. |
UniProt Protein Details: | Protein type:Secreted; Secreted, signal peptide Chromosomal Location of Human Ortholog: 5p13.1-p12 Cellular Component: extracellular region Molecular Function:protein homodimerization activity; growth factor activity; receptor binding Biological Process: positive regulation of dopamine secretion; axon guidance; nervous system development; peristalsis; adult locomotory behavior; mRNA stabilization; regulation of dopamine uptake; positive regulation of monooxygenase activity; signal transduction; enteric nervous system development; sympathetic nervous system development; ureteric bud branching; regulation of gene expression; induction of an organ; positive regulation of cell proliferation; postganglionic parasympathetic nervous system development; positive regulation of transcription from RNA polymerase II promoter; negative regulation of neuron apoptosis; positive regulation of cell differentiation; postsynaptic membrane organization; metanephros development; neural crest cell migration; neurite development; negative regulation of apoptosis Disease: Hirschsprung Disease, Susceptibility To, 3; Central Hypoventilation Syndrome, Congenital; Pheochromocytoma |
NCBI Summary: | This gene encodes a highly conserved neurotrophic factor. The recombinant form of this protein was shown to promote the survival and differentiation of dopaminergic neurons in culture, and was able to prevent apoptosis of motor neurons induced by axotomy. The encoded protein is processed to a mature secreted form that exists as a homodimer. The mature form of the protein is a ligand for the product of the RET (rearranged during transfection) protooncogene. Multiple transcript variants encoding different isoforms have been found for this gene. Mutations in this gene may be associated with Hirschsprung disease. [provided by RefSeq, Jun 2010] |
UniProt Code: | P39905 |
NCBI GenInfo Identifier: | 729567 |
NCBI Gene ID: | 2668 |
NCBI Accession: | P39905.1 |
UniProt Secondary Accession: | P39905,O95448, O95449, O95986, Q6FH33, Q96L44, Q9UD32 Q9UD33, Q9UMV2, Q9UP67, Q9UP97, B7WPK7, |
UniProt Related Accession: | P39905,AAB33493,AAB33494 |
Molecular Weight: | 18,123 Da |
NCBI Full Name: | Glial cell line-derived neurotrophic factor |
NCBI Synonym Full Names: | glial cell derived neurotrophic factor |
NCBI Official Symbol: | GDNFÂ Â |
NCBI Official Synonym Symbols: | ATF1; ATF2; HSCR3; HFB1-GDNFÂ Â |
NCBI Protein Information: | glial cell line-derived neurotrophic factor; ATF; astrocyte-derived trophic factor |
UniProt Protein Name: | Glial cell line-derived neurotrophic factor |
UniProt Synonym Protein Names: | Astrocyte-derived trophic factor; ATF |
Protein Family: | GDNF family receptor |
UniProt Gene Name: | GDNFÂ Â |
UniProt Entry Name: | GDNF_HUMAN |