Anti-FUBP1 Antibody (RACO0261)
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주문- SKU:
- RACO0261
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- IP
- Application:
- WB
- Application:
- IF
- Application:
- ELISA
- Application:
- IHC
- Application:
- FC
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Product Name: | FUBP1 Antibody |
Product SKU: | RACO0261 |
Size: | 50ul |
Host Species: | Homo sapiens (Human) |
Tested Applications: | ELISA, WB, IHC, IF, FC, IP |
Recommended Dilutions: | WB:1:500-1:5000, IHC:1:50-1:200, IF:1:20-1:200, FC:1:20-1:200, IP:1:200-1:1000 |
Species Reactivity: | Human |
Immunogen: | A synthesized peptide derived from human FUBP1 |
Form: | Liquid |
Storage Buffer: | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Purification Method: | Affinity-chromatography |
Clonality: | Monoclonal |
Isotype: | Rabbit IgG |
Conjugate: | Non-conjugated |
Background: | Regulates MYC expression by binding to a single-stranded far-upstream element (FUSE) upstream of the MYC promoter. May act both as activator and repressor of transcription. |
Synonyms: | Far upstream element-binding protein 1 (FBP) (FUSE-binding protein 1) (DNA helicase V) (hDH V), FUBP1 |
IHC image of RACO0261 diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. | |
IHC image of RACO0261 diluted at 1:100 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. | |
Western Blot. Positive WB detected in Jurkat whole cell lysate K562 whole cell lysate Hela whole cell lysate Raji whole cell lysate HepG2 whole cell lysate All lanes FUBP1 antibody at 1:2000. Secondary. Goat polyclonal to rabbit IgG at 1:50000 dilution. Predicted band size: 68, 69 kDa. Observed band size: 69 kDa. |
UniProt Protein Function: | FBP1: Regulates MYC expression by binding to a single-stranded far-upstream element (FUSE) upstream of the MYC promoter. May act both as activator and repressor of transcription. 2 isoforms of the human protein are produced by alternative splicing. |
UniProt Protein Details: | Protein type:DNA-binding; Transcription factor; RNA-binding; Motility/polarity/chemotaxis; RNA splicing Chromosomal Location of Human Ortholog: 1p31.1 Cellular Component: nucleoplasm; nucleus Molecular Function:protein binding; transcription factor activity; single-stranded DNA binding Biological Process: transcription from RNA polymerase II promoter; positive regulation of transcription from RNA polymerase II promoter |
NCBI Summary: | The protein encoded by this gene is a single stranded DNA-binding protein that binds to multiple DNA elements, including the far upstream element (FUSE) located upstream of c-myc. Binding to FUSE occurs on the non-coding strand, and is important to the regulation of c-myc in undifferentiated cells. This protein contains three domains, an amphipathic helix N-terminal domain, a DNA-binding central domain, and a C-terminal transactivation domain that contains three tyrosine-rich motifs. The N-terminal domain is thought to repress the activity of the C-terminal domain. This protein is also thought to bind RNA, and contains 3'-5' helicase activity with in vitro activity on both DNA-DNA and RNA-RNA duplexes. Aberrant expression of this gene has been found in malignant tissues, and this gene is important to neural system and lung development. Binding of this protein to viral RNA is thought to play a role in several viral diseases, including hepatitis C and hand, foot and mouth disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014] |
UniProt Code: | Q96AE4 |
NCBI GenInfo Identifier: | 116241370 |
NCBI Gene ID: | 8880 |
NCBI Accession: | Q96AE4.3 |
UniProt Secondary Accession: | Q96AE4,Q12828, |
UniProt Related Accession: | Q96AE4 |
Molecular Weight: | 653 |
NCBI Full Name: | Far upstream element-binding protein 1 |
NCBI Synonym Full Names: | far upstream element (FUSE) binding protein 1 |
NCBI Official Symbol: | FUBP1Â Â |
NCBI Official Synonym Symbols: | FBP; FUBP; hDH VÂ Â |
NCBI Protein Information: | far upstream element-binding protein 1; DNA helicase V |
UniProt Protein Name: | Far upstream element-binding protein 1 |
UniProt Synonym Protein Names: | DNA helicase V; hDH V |
Protein Family: | Far upstream element-binding protein |
UniProt Gene Name: | FUBP1Â Â |
UniProt Entry Name: | FUBP1_HUMAN |