Anti-FOS Antibody (RACO0205)
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주문- SKU:
- RACO0205
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- WB
- Application:
- IF
- Application:
- ELISA
- Application:
- IHC
- Application:
- FC
- Conjugation:
- Unconjugated
Description
Product Name: | FOS Antibody |
Product SKU: | RACO0205 |
Size: | 50ul |
Host Species: | Human |
Tested Applications: | ELISA, WB, IHC, IF, FC |
Recommended Dilutions: | WB:1:500-1:5000, IHC:1:50-1:200, IF:1:20-1:200 |
Species Reactivity: | Human, Mouse |
Immunogen: | A synthesized peptide derived from human FOS |
Form: | Liquid |
Storage Buffer: | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Purification Method: | Affinity-chromatography |
Clonality: | Monoclonal |
Isotype: | Rabbit IgG |
Conjugate: | Non-conjugated |
Background: | Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation. In growing cells, activates phospholipid synthesis, possibly by activating CDS1 and PI4K2A. This activity requires Tyr-dephosphorylation and association with the endoplasmic reticulum. |
Synonyms: | Proto-oncogene c-Fos, Cellular oncogene fos, G0/G1 switch regulatory protein 7, FOS, G0S7 |
IHC image of RACO0205 diluted at 1:81 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. | |
IHC image of RACO0205 diluted at 1:81 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. | |
Western Blot. Positive WB detected in HepG2 whole cell lysate Hela whole cell lysate MCF-7 whole cell lysate Jurkat whole cell lysate A549 whole cell lysate 293 whole cell lysate NIH/3T3 whole cell lysate All lanes c-FOS antibody at 0. (1µg) ml. Secondary. Goat polyclonal to rabbit IgG at 1:50000 dilution. Predicted band size: 41, 29, 37 KDa. Observed band size: 62 KDa. |
UniProt Protein Function: | Fos: a proto-oncogenic transcription factor of the bZIP family. Dimerizes with proteins of the JUN family, thereby forming the transcription factor complex AP-1. FOS proteins function as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of FOS has also been associated with apoptotic cell death. Expression increases upon a variety of stimuli, including growth factors, cytokines, neurotransmitters, polypeptide hormones, stress and cell injury. |
UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; DNA-binding; Transcription factor; Oncoprotein Chromosomal Location of Human Ortholog: 14q24.3 Cellular Component: nucleoplasm; transcription factor complex; neuron projection; membrane; endoplasmic reticulum; nucleus; cytosol Molecular Function:protein binding; double-stranded DNA binding; transcription factor activity; transcription factor binding Biological Process: transcription from RNA polymerase II promoter; response to gravity; response to cAMP; positive regulation of osteoclast differentiation; positive regulation of transcription, DNA-dependent; response to toxin; stress-activated MAPK cascade; response to lipopolysaccharide; toll-like receptor 3 signaling pathway; female pregnancy; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; regulation of transcription factor activity; transforming growth factor beta receptor signaling pathway; conditioned taste aversion; DNA methylation; inflammatory response; toll-like receptor 4 signaling pathway; aging; response to corticosterone stimulus; response to drug; response to light stimulus; nervous system development; MyD88-independent toll-like receptor signaling pathway; sleep; toll-like receptor 2 signaling pathway; cellular response to hormone stimulus; regulation of transcription from RNA polymerase II promoter; MyD88-dependent toll-like receptor signaling pathway; response to mechanical stimulus; response to cytokine stimulus; cellular response to extracellular stimulus; toll-like receptor signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter; response to cold; toll-like receptor 9 signaling pathway; response to progesterone stimulus |
NCBI Summary: | The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death. [provided by RefSeq, Jul 2008] |
UniProt Code: | P01100 |
NCBI GenInfo Identifier: | 120470 |
NCBI Gene ID: | 2353 |
NCBI Accession: | P01100.1 |
UniProt Secondary Accession: | P01100,P18849, A8K4E2, B4DQ65, |
UniProt Related Accession: | P01100 |
Molecular Weight: | 380 |
NCBI Full Name: | Proto-oncogene c-Fos |
NCBI Synonym Full Names: | FBJ murine osteosarcoma viral oncogene homolog |
NCBI Official Symbol: | FOSÂ Â |
NCBI Official Synonym Symbols: | p55; AP-1; C-FOSÂ Â |
NCBI Protein Information: | proto-oncogene c-Fos; activator protein 1; cellular oncogene c-fos; G0/G1 switch regulatory protein 7; FBJ murine osteosarcoma viral (v-fos) oncogene homolog (oncogene FOS) |
UniProt Protein Name: | Proto-oncogene c-Fos |
UniProt Synonym Protein Names: | Cellular oncogene fos; G0/G1 switch regulatory protein 7 |
Protein Family: | Fos-related antigen |
UniProt Gene Name: | FOSÂ Â |
UniProt Entry Name: | FOS_HUMAN |