Anti-EIF4A1 Antibody (RACO0501)
제품 코드를 사용하여 Assay Genie 메인 사이트를 통해 주문합니다.
주문- SKU:
- RACO0501
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- WB
- Application:
- IF
- Application:
- ELISA
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Product Name: | EIF4A1 Antibody |
Product SKU: | RACO0501 |
Size: | 50ul |
Host Species: | Homo sapiens (Human) |
Tested Applications: | ELISA, WB, IF |
Recommended Dilutions: | WB:1:500-1:5000, IF:1:20-1:200 |
Species Reactivity: | Human |
Immunogen: | A synthesized peptide derived from human eIF4A1 |
Form: | Liquid |
Storage Buffer: | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Purification Method: | Affinity-chromatography |
Clonality: | Monoclonal |
Isotype: | Rabbit IgG |
Conjugate: | Non-conjugated |
Background: | ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon. |
Synonyms: | Eukaryotic initiation factor 4A-I (eIF-4A-I) (eIF4A-I) (EC 3.6.4.13) (ATP-dependent RNA helicase eIF4A-1), EIF4A1, DDX2A EIF4A |
Immunofluorescence staining of Hela Cells with RACO0501 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L). | |
Western Blot. Positive WB detected in K562 whole cell lysate 293 whole cell lysate RAW264.7 whole cell lysate All lanes eIF4A1 Antibody at 1:1000. Secondary. Goat polyclonal to rabbit IgG at 1:50000 dilution. Predicted band size: 40, 47 kDa. Observed band size: 50 kDa. |
UniProt Protein Function: | ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon. |
UniProt Code: | P60842 |
NCBI GenInfo Identifier: | 46397463 |
NCBI Gene ID: | 1973 |
NCBI Accession: | P60842.1 |
UniProt Secondary Accession: | P60842,P04765, Q5U018, Q61516, B2R6L8, D3DTP9, J3QLC4 |
UniProt Related Accession: | P60842 |
Molecular Weight: | 39,548 Da |
NCBI Full Name: | Eukaryotic initiation factor 4A-I |
NCBI Synonym Full Names: | eukaryotic translation initiation factor 4A1 |
NCBI Official Symbol: | EIF4A1Â Â |
NCBI Official Synonym Symbols: | DDX2A; EIF4A; EIF-4A; eIF4A-I; eIF-4A-IÂ Â |
NCBI Protein Information: | eukaryotic initiation factor 4A-I |
UniProt Protein Name: | Eukaryotic initiation factor 4A-I |
UniProt Synonym Protein Names: | ATP-dependent RNA helicase eIF4A-1 |
Protein Family: | Eukaryotic initiation factor |
UniProt Gene Name: | EIF4A1Â Â |
UniProt Entry Name: | IF4A1_HUMAN |