Anti-DOT1L Antibody (RACO0228)
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주문- SKU:
- RACO0228
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- WB
- Application:
- ELISA
- Conjugation:
- Unconjugated
Description
Product Name: | DOT1L Antibody |
Product SKU: | RACO0228 |
Size: | 50ul |
Host Species: | Homo sapiens (Human) |
Tested Applications: | ELISA, WB |
Recommended Dilutions: | WB:1:500-1:5000 |
Species Reactivity: | Human |
Immunogen: | A synthesized peptide derived from human KMT4 / Dot1L |
Form: | Liquid |
Storage Buffer: | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Purification Method: | Affinity-chromatography |
Clonality: | Monoclonal |
Isotype: | Rabbit IgG |
Conjugate: | Non-conjugated |
Background: | Histone methyltransferase. Methylates 'Lys-79' of histone H3. Nucleosomes are preferred as substrate compared to free histones. Binds to DNA. |
Synonyms: | Histone-lysine N-methyltransferase, H3 lysine-79 specific (EC 2.1.1.43) (DOT1-like protein) (Histone H3-K79 methyltransferase) (H3-K79-HMTase) (Lysine N-methyltransferase 4), DOT1L, KIAA1814 KMT4 |
Western Blot. Positive WB detected in Hela whole cell lysate HL60 whole cell lysate K562 whole cell lysate SH-SY5Y whole cell lysate U251 whole cell lysate PC-3 whole cell lysate All lanes DOT1L antibody at 1:1500. Secondary. Goat polyclonal to rabbit IgG at 1:50000 dilution. Predicted band size: 165, 185 kDa. Observed band size: 185 kDa. |
UniProt Protein Function: | DOT1L: a protein-lysine N-methyltransferase of the seven beta-strand class. Catalyzes the mono-, di- and tri-methylation of H3K79. Methylated H3K79 is associated with active gene transcription. DOT1L activity drives proliferation of mixed lineage leukemia 1 protein (MLL)-rearranged leukemias, inducing the aberrant transcription of a number of leukemogenic genes including HOXA9 and MEIS1. Binds to DNA. Belongs to the class I-like SAM-binding methyltransferase superfamily. DOT1 family. 2 isoforms of the human protein are produced by alternative splicing. |
UniProt Protein Details: | Protein type:Amino Acid Metabolism - lysine degradation; EC 2.1.1.43; Methyltransferase; Methyltransferase, protein lysine Chromosomal Location of Human Ortholog: 19p13.3 Cellular Component: intracellular membrane-bound organelle; nucleoplasm; nucleus; protein complex Molecular Function:histone lysine N-methyltransferase activity (H3-K79 specific); histone methyltransferase activity; histone-lysine N-methyltransferase activity; protein binding Biological Process: chromatin silencing at telomere; DNA damage checkpoint; DNA repair; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; regulation of JAK-STAT cascade; telomere organization and biogenesis |
NCBI Summary: | The protein encoded by this gene is a histone methyltransferase that methylates lysine-79 of histone H3. It is inactive against free core histones, but shows significant histone methyltransferase activity against nucleosomes. [provided by RefSeq, Aug 2011] |
UniProt Code: | Q8TEK3 |
NCBI GenInfo Identifier: | 25090171 |
NCBI Gene ID: | 84444 |
NCBI Accession: | Q8TEK3.2 |
UniProt Secondary Accession: | Q8TEK3,O60379, Q96JL1, |
UniProt Related Accession: | Q8TEK3 |
Molecular Weight: | 185kDa |
NCBI Full Name: | Histone-lysine N-methyltransferase, H3 lysine-79 specific |
NCBI Synonym Full Names: | DOT1 like histone lysine methyltransferase |
NCBI Official Symbol: | DOT1LÂ Â |
NCBI Official Synonym Symbols: | DOT1; KMT4Â Â |
NCBI Protein Information: | histone-lysine N-methyltransferase, H3 lysine-79 specific |
UniProt Protein Name: | Histone-lysine N-methyltransferase, H3 lysine-79 specific |
UniProt Synonym Protein Names: | DOT1-like protein; Histone H3-K79 methyltransferase; H3-K79-HMTase; Lysine N-methyltransferase 4 |
UniProt Gene Name: | DOT1LÂ Â |