Anti-CDC5L Antibody (RACO0143)
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주문- SKU:
- RACO0143
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- IP
- Application:
- WB
- Application:
- IF
- Application:
- ELISA
- Application:
- IHC
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Product Name: | CDC5L Antibody |
Product SKU: | RACO0143 |
Size: | 50ul |
Host Species: | Human |
Tested Applications: | ELISA, WB, IHC, IF, IP |
Recommended Dilutions: | WB:1:500-1:5000, IHC:1:50-1:200, IF:1:20-1:200, IP:1:200-1:1000 |
Species Reactivity: | Human |
Immunogen: | A synthesized peptide derived from human CDC5L |
Form: | Liquid |
Storage Buffer: | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Purification Method: | Affinity-chromatography |
Clonality: | Monoclonal |
Isotype: | Rabbit IgG |
Conjugate: | Non-conjugated |
Background: | DNA-binding protein involved in cell cycle control. May act as a transcription activator. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. The PRP19-CDC5L complex may also play a role in the response to DNA damage (DDR). |
Synonyms: | Cell division cycle 5-like protein, Cdc5-like protein, Pombe cdc5-related protein, CDC5L, KIAA0432, PCDC5RP |
IHC image of RACO0143 diluted at 1:126.5 and staining in paraffin-embedded human appendix tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. | |
IHC image of RACO0143 diluted at 1:126.5 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. | |
Western Blot. Positive WB detected in Hela whole cell lysate MCF-7 whole cell lysate HepG2 whole cell lysate 293 whole cell lysate K562 whole cell lysate Jurkat whole cell lysate All lanes CDC5L antibody at 1 (3µg) ml. Secondary. Goat polyclonal to rabbit IgG at 1:50000 dilution. Predicted band size: 93 KDa. Observed band size: 100 KDa. |
UniProt Protein Function: | CDC5L: is a spliceosome protein that binds DNA and that also regulates transcription. Is a positive regulator of cell cycle G2/M progression. Is an essential component of non-snRNA spliceosomes and is required for the second catalytic step of pre-mRNA splicing. Binds adeno-pre-mRNA in an ATP-stimulated manner. Is part of a spliceosomal 'core' complex that includes CDC5L, SF2, PRLI, SPF27, HSP7C, PRPF19/SNEV, GCN1L. Identified in the spliceosome C complex. Has been associated with aneuploidy in aging cells and in tumorigenesis. The genomic region containing the gene for CDC5L (6p21.1) is frequently amplified in osteosarcomas. |
UniProt Protein Details: | Protein type:DNA-binding; RNA processing; Transcription factor; Spliceosome; Motility/polarity/chemotaxis; RNA splicing Chromosomal Location of Human Ortholog: 6p21 Cellular Component: nucleoplasm; DNA replication factor A complex; membrane; cytoplasm; nucleolus; nuclear speck; nucleus Molecular Function:protein binding; DNA binding; chromatin binding Biological Process: nuclear mRNA splicing, via spliceosome; transcription, DNA-dependent; regulation of transcription, DNA-dependent; RNA splicing; gene expression; DNA repair |
NCBI Summary: | The protein encoded by this gene shares a significant similarity with Schizosaccharomyces pombe cdc5 gene product, which is a cell cycle regulator important for G2/M transition. This protein has been demonstrated to act as a positive regulator of cell cycle G2/M progression. It was also found to be an essential component of a non-snRNA spliceosome, which contains at least five additional protein factors and is required for the second catalytic step of pre-mRNA splicing. [provided by RefSeq, Jul 2008] |
UniProt Code: | Q99459 |
NCBI GenInfo Identifier: | 73619933 |
NCBI Gene ID: | 988 |
NCBI Accession: | Q99459.2 |
UniProt Related Accession: | Q99459 |
Molecular Weight: | 100kDa |
NCBI Full Name: | Cell division cycle 5-like protein |
NCBI Synonym Full Names: | cell division cycle 5 like |
NCBI Official Symbol: | CDC5LÂ Â |
NCBI Official Synonym Symbols: | CDC5; CEF1; PCDC5RP; CDC5-LIKE; dJ319D22.1Â Â |
NCBI Protein Information: | cell division cycle 5-like protein |
UniProt Protein Name: | Cell division cycle 5-like protein |
UniProt Synonym Protein Names: | Pombe cdc5-related protein |
Protein Family: | CDC50 family protein |
UniProt Gene Name: | CDC5LÂ Â |
UniProt Entry Name: | CDC5L_HUMAN |