Anti-CD9 Antibody (RACO0011)
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주문- SKU:
- RACO0011
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- WB
- Application:
- IF
- Application:
- ELISA
- Application:
- IHC
- Application:
- FC
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Product Name: | CD9 Antibody |
Product SKU: | RACO0011 |
Size: | 50ul |
Host Species: | Human |
Tested Applications: | ELISA, WB, IHC, IF, FC |
Recommended Dilutions: | WB:1:500-1:5000, IHC:1:50-1:500, IF:1:30-1:200 |
Species Reactivity: | Human |
Immunogen: | A synthesized peptide |
Form: | Liquid |
Storage Buffer: | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Purification Method: | Affinity-chromatography |
Clonality: | Monoclonal |
Isotype: | Rabbit IgG |
Conjugate: | Non-conjugated |
Background: | Involved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis. Required for sperm-egg fusion. |
Synonyms: | CD9 antigen, 5H9 antigen, Cell growth-inhibiting gene 2 protein, Leukocyte antigen MIC3, Motility-related protein, MRP-1, Tetraspanin-29, Tspan-29, p24, CD9, CD9, MIC3, TSPAN29, GIG2 |
IHC image of RACO0011 diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. | |
Immunofluorescence staining of MCF-7 cells with RACO0011 at 1:34,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L). | |
Western Blot. Positive WB detected in U87 whole cell lysate All lanes CD9 antibody at 0.55µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1:50000 dilution. Predicted band size: 25 KDa. Observed band size: 25 KDa. |
UniProt Protein Function: | CD9: Involved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis. Required for sperm-egg fusion. Belongs to the tetraspanin (TM4SF) family. |
UniProt Protein Details: | Protein type:Cell adhesion; Membrane protein, integral; Membrane protein, multi-pass; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 12p13.31 Cellular Component: extracellular space; focal adhesion; integral to plasma membrane; membrane; plasma membrane; platelet alpha granule membrane Molecular Function:protein binding Biological Process: cell adhesion; cell motility; cell surface receptor linked signal transduction; fusion of sperm to egg plasma membrane; paranodal junction assembly; platelet degranulation; receptor internalization |
NCBI Summary: | This gene encodes a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Tetraspanins are cell surface glycoproteins with four transmembrane domains that form multimeric complexes with other cell surface proteins. The encoded protein functions in many cellular processes including differentiation, adhesion, and signal transduction, and expression of this gene plays a critical role in the suppression of cancer cell motility and metastasis. [provided by RefSeq, Jan 2011] |
UniProt Code: | P21926 |
NCBI GenInfo Identifier: | 231724 |
NCBI Gene ID: | 928 |
NCBI Accession: | P21926.4 |
UniProt Secondary Accession: | P21926,Q5J7W6, Q96ES4, D3DUQ9, |
UniProt Related Accession: | P21926 |
Molecular Weight: | |
NCBI Full Name: | CD9 antigen |
NCBI Synonym Full Names: | CD9 molecule |
NCBI Official Symbol: | CD9Â Â |
NCBI Official Synonym Symbols: | MIC3; MRP-1; BTCC-1; DRAP-27; TSPAN29; TSPAN-29Â Â |
NCBI Protein Information: | CD9 antigen |
UniProt Protein Name: | CD9 antigen |
UniProt Synonym Protein Names: | 5H9 antigen; Cell growth-inhibiting gene 2 protein; Leukocyte antigen MIC3; Motility-related protein; MRP-1; Tetraspanin-29; Tspan-29; p24; CD_antigen: CD9 |
Protein Family: | CD9 antigen |
UniProt Gene Name: | CD9Â Â |