Anti-CD4 Antibody (RACO0030)
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주문- SKU:
- RACO0030
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- WB
- Application:
- ELISA
- Application:
- IHC
- Conjugation:
- Unconjugated
Description
Product Name: | CD4 Antibody |
Product SKU: | RACO0030 |
Size: | 50ul |
Host Species: | Human |
Tested Applications: | ELISA, WB, IHC |
Recommended Dilutions: | WB:1:500-1:5000, IHC:1:50-1:500 |
Species Reactivity: | Human |
Immunogen: | A synthesized peptide |
Form: | Liquid |
Storage Buffer: | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Purification Method: | Affinity-chromatography |
Clonality: | Monoclonal |
Isotype: | Rabbit IgG |
Conjugate: | Non-conjugated |
Background: | Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts. |
Synonyms: | T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3, CD4, CD4 |
IHC image of RACO0030 diluted at 1:100 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. | |
IHC image of RACO0030 diluted at 1:100 and staining in paraffin-embedded human spleen tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. | |
Western Blot. Positive WB detected in Jurkat whole cell lysate All lanes CD4 antibody at 0.8µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1:50000 dilution. Predicted band size: 55 KDa. Observed band size: 55 KDa. |
UniProt Protein Function: | CD4: Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts. Associates with LCK. Binds to HIV-1 gp120 and to P4HB/PDI and upon HIV-1 binding to the cell membrane, is part of P4HB/PDI- CD4-CXCR4-gp120 complex. Interacts with HIV-1 Envelope polyprotein gp160 and protein Vpu. Interacts with Human Herpes virus 7 capsid proteins. Interacts with PTK2/FAK1; this interaction requires the presence of HIV-1 gp120. |
UniProt Protein Details: | Protein type:Membrane protein, integral; Cell surface Chromosomal Location of Human Ortholog: 12p13.31 Cellular Component: T cell receptor complex; endoplasmic reticulum membrane; endoplasmic reticulum lumen; early endosome; plasma membrane; integral to membrane; external side of plasma membrane; lipid raft Molecular Function:protein binding; enzyme binding; transmembrane receptor activity; protein homodimerization activity; zinc ion binding; extracellular matrix structural constituent; coreceptor activity; receptor activity; glycoprotein binding; protein kinase binding; MHC class II protein binding Biological Process: maintenance of cellular protein localization; viral reproduction; positive regulation of interleukin-2 biosynthetic process; cytokine production; T cell selection; positive regulation of calcium-mediated signaling; defense response to Gram-negative bacterium; signal transduction; induction by virus of cell-cell fusion in host; T cell receptor signaling pathway; enzyme linked receptor protein signaling pathway; regulation of defense response to virus by virus; positive regulation of peptidyl-tyrosine phosphorylation; cell surface receptor linked signal transduction; positive regulation of protein kinase activity; T cell costimulation; protein palmitoleylation; innate immune response; entry into host cell; immune response; transmembrane receptor protein tyrosine kinase signaling pathway; T cell differentiation; regulation of T cell activation Disease: Okt4 Epitope Deficiency |
NCBI Summary: | This gene encodes a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigenes and is also a receptor for the human immunodeficiency virus. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene. [provided by RefSeq, Aug 2010] |
UniProt Code: | P01730 |
NCBI GenInfo Identifier: | 116013 |
NCBI Gene ID: | 920 |
NCBI Accession: | P01730.1 |
UniProt Secondary Accession: | P01730,Q4ZGK2, Q5U066, Q9UDE5, B2R737, D3DUS5, |
UniProt Related Accession: | P01730 |
Molecular Weight: | 458 |
NCBI Full Name: | T-cell surface glycoprotein CD4 |
NCBI Synonym Full Names: | CD4 molecule |
NCBI Official Symbol: | CD4Â Â |
NCBI Official Synonym Symbols: | CD4mut  |
NCBI Protein Information: | T-cell surface glycoprotein CD4; CD4 receptor; CD4 antigen (p55); T-cell surface antigen T4/Leu-3 |
UniProt Protein Name: | T-cell surface glycoprotein CD4 |
UniProt Synonym Protein Names: | T-cell surface antigen T4/Leu-3; CD_antigen: CD4 |
Protein Family: | CD40 ligand |
UniProt Gene Name: | CD4Â Â |
UniProt Entry Name: | CD4_HUMAN |