Anti-CD19 Antibody (RACO0397)
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주문- SKU:
- RACO0397
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- IP
- Application:
- WB
- Application:
- ELISA
- Application:
- IHC
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Product Name: | CD19 Antibody |
Product SKU: | RACO0397 |
Size: | 50ul |
Host Species: | Homo sapiens (Human) |
Tested Applications: | ELISA, WB, IHC, IP |
Recommended Dilutions: | WB:1:500-1:5000, IHC:1:50-1:200, IP:1:200-1:1000 |
Species Reactivity: | Human |
Immunogen: | A synthesized peptide derived from human CD19 |
Form: | Liquid |
Storage Buffer: | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Purification Method: | Affinity-chromatography |
Clonality: | Monoclonal |
Isotype: | Rabbit IgG |
Conjugate: | Non-conjugated |
Background: | Assembles with the antigen receptor of B-lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. |
Synonyms: | B-lymphocyte antigen CD19 (B-lymphocyte surface antigen B4) (Differentiation antigen CD19) (T-cell surface antigen Leu-12) (CD antigen CD19), CD19 |
IHC image of RACO0397 diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. | |
Immunoprecipitating CD19 in Raji whole cell lysate Lane 1: Rabbit control IgG instead of RACO0397 in Raji whole cell lysate For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: RACO0397(2µg)+ Raji whole cell lysate (500µg). Lane 3: Raji whole cell lysate (10µg). | |
Western Blot. Positive WB detected in Daudi whole cell lysate Raji whole cell lysate All lanes CD19 antibody at 1:2000. Secondary. Goat polyclonal to rabbit IgG at 1:50000 dilution. Predicted band size: 62 kDa. Observed band size: 95 kDa. |
UniProt Protein Function: | Assembles with the antigen receptor of B-lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. |
NCBI Summary: | Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. This gene encodes a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. [provided by RefSeq, Jul 2008] |
UniProt Code: | P15391 |
NCBI GenInfo Identifier: | 160332376 |
NCBI Gene ID: | 930 |
NCBI Accession: | P15391.6 |
UniProt Secondary Accession: | P15391,Q96S68, Q9BRD6, A0N0P9, F5H635, |
UniProt Related Accession: | P15391 |
Molecular Weight: | |
NCBI Full Name: | B-lymphocyte antigen CD19 |
NCBI Synonym Full Names: | CD19 molecule |
NCBI Official Symbol: | CD19Â Â |
NCBI Official Synonym Symbols: | B4; CVID3Â Â |
NCBI Protein Information: | B-lymphocyte antigen CD19 |
UniProt Protein Name: | B-lymphocyte antigen CD19 |
UniProt Synonym Protein Names: | B-lymphocyte surface antigen B4; Differentiation antigen CD19; T-cell surface antigen Leu-12; CD_antigen: CD19 |
Protein Family: | B-lymphocyte antigen |
UniProt Gene Name: | CD19Â Â |