Anti-CCR9 Antibody (RACO0160)
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주문- SKU:
- RACO0160
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- IP
- Application:
- WB
- Application:
- IF
- Application:
- ELISA
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Product Name: | CCR9 Antibody |
Product SKU: | RACO0160 |
Size: | 50ul |
Host Species: | Human |
Tested Applications: | ELISA, WB, IF, IP |
Recommended Dilutions: | WB:1:500-1:5000, IF:1:20-1:200, IP:1:200-1:1000 |
Species Reactivity: | Human, Mouse |
Immunogen: | A synthesized peptide derived from human CCR9 |
Form: | Liquid |
Storage Buffer: | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Purification Method: | Affinity-chromatography |
Clonality: | Monoclonal |
Isotype: | Rabbit IgG |
Conjugate: | Non-conjugated |
Background: | Receptor for chemokine SCYA25/TECK. Subsequently transduces a signal by increasing the intracellular calcium ions level. |
Synonyms: | C-C chemokine receptor type 9, C-C CKR-9, CC-CKR-9, CCR-9, G-protein coupled receptor 28, GPR-9-6, CDw199, CCR9, GPR28 |
Immunofluorescence staining of HepG2 cells with RACO0160 at 1:33, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L). | |
Immunoprecipitating CCR9 in HL-60 whole cell lysate Lane 1: Rabbit control IgG instead of RACO0160 in HL-60 whole cell lysate For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: RACO0160 (3µg) + HL-60 whole cell lysate (500µg). Lane 3: HL-60 whole cell lysate (20µg). | |
Western Blot. Positive WB detected in SH-SY5Y whole cell lysate HepG2 whole cell lysate Jurkat whole cell lysate HL-60 whole cell lysate Hela whole cell lysate U87 whole cell lysate Mouse brain tissue. All lanes CCR9 antibody at (1µg) ml. Secondary. Goat polyclonal to rabbit IgG at 1:50000 dilution. Predicted band size: 43, 41 KDa. Observed band size: 43 KDa. |
UniProt Protein Function: | CCR9: Receptor for chemokine SCYA25/TECK. Subsequently transduces a signal by increasing the intracellular calcium ions level. Alternative coreceptor with CD4 for HIV-1 infection. Belongs to the G-protein coupled receptor 1 family. 2 isoforms of the human protein are produced by alternative splicing. |
UniProt Protein Details: | Protein type:Membrane protein, integral; Motility/polarity/chemotaxis; Receptor, GPCR; Membrane protein, multi-pass; GPCR, family 1 Chromosomal Location of Human Ortholog: 3p21.3 Cellular Component: cell surface; integral to plasma membrane; plasma membrane Molecular Function:C-C chemokine receptor activity; chemokine receptor activity Biological Process: G-protein coupled receptor protein signaling pathway; elevation of cytosolic calcium ion concentration; cellular defense response; immune response; chemotaxis |
NCBI Summary: | The protein encoded by this gene is a member of the beta chemokine receptor family. It is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. Chemokines and their receptors are key regulators of the thymocytes migration and maturation in normal and inflammation conditions. The specific ligand of this receptor is CCL25. It has been found that this gene is differentially expressed by T lymphocytes of small intestine and colon, suggested a role in the thymocytes recruitment and development that may permit functional specialization of immune responses in different segment of the gastrointestinal tract. This gene is mapped to the chemokine receptor gene cluster region. Two alternatively spliced transcript variants have been described. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012] |
UniProt Code: | P51686 |
NCBI GenInfo Identifier: | 114152781 |
NCBI Gene ID: | 10803 |
NCBI Accession: | P51686.2 |
UniProt Secondary Accession: | P51686,Q9WUT7, |
UniProt Related Accession: | P51686 |
Molecular Weight: | 40,713 Da |
NCBI Full Name: | C-C chemokine receptor type 9 |
NCBI Synonym Full Names: | chemokine (C-C motif) receptor 9 |
NCBI Official Symbol: | CCR9Â Â |
NCBI Official Synonym Symbols: | GPR28; CDw199; GPR-9-6; CC-CKR-9Â Â |
NCBI Protein Information: | C-C chemokine receptor type 9; G protein-coupled receptor 28 |
UniProt Protein Name: | C-C chemokine receptor type 9 |
UniProt Synonym Protein Names: | G-protein coupled receptor 28; GPR-9-6; CD_antigen: CDw199 |
Protein Family: | C-C chemokine receptor |
UniProt Gene Name: | CCR9Â Â |
UniProt Entry Name: | CCR9_HUMAN |