Anti-CCND1 Antibody (RACO0566)
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주문- SKU:
- RACO0566
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- WB
- Application:
- ELISA
- Conjugation:
- Unconjugated
Description
Product Name: | CCND1 Antibody |
Product SKU: | RACO0566 |
Size: | 50ul |
Host Species: | Homo sapiens (Human) |
Tested Applications: | ELISA, WB |
Recommended Dilutions: | WB:1:500-1:5000 |
Species Reactivity: | Human, Mouse |
Immunogen: | A synthesized peptide derived from human Cyclin D1 |
Form: | Liquid |
Storage Buffer: | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Purification Method: | Affinity-chromatography |
Clonality: | Monoclonal |
Isotype: | Rabbit IgG |
Conjugate: | Non-conjugated |
Background: | Regulatory component of the cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D1/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. Exhibits transcriptional corepressor activity with INSM1 on the NEUROD1 and INS promoters in a cell cycle-independent manner. |
Synonyms: | G1/S-specific cyclin-D1 (B-cell lymphoma 1 protein) (BCL-1) (BCL-1 oncogene) (PRAD1 oncogene), CCND1, BCL1 PRAD1 |
Western Blot. Positive WB detected in NIH/3T3 whole cell lysate SH-SY5Y whole cell lysate U-87 whole cell lysate All lanes Cyclin D1 antibody at 1:1000. Secondary. Goat polyclonal to rabbit IgG at 1:50000 dilution. Predicted band size: 34 kDa. Observed band size: 34 kDa. |
UniProt Protein Function: | CCND1: a member of the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. |
UniProt Protein Details: | Protein type:Activator; Cell cycle regulation; Nuclear receptor co-regulator; Oncoprotein Chromosomal Location of Human Ortholog: 11q13.3 Cellular Component: cyclin-dependent protein kinase holoenzyme complex; cytosol; intracellular; nucleoplasm; nucleus; transcriptional repressor complex Molecular Function:enzyme binding; histone deacetylase binding; protein binding; protein kinase binding; transcription corepressor activity; transcription factor binding Biological Process: G1 DNA damage checkpoint; G1/S transition of mitotic cell cycle; negative regulation of transcription from RNA polymerase II promoter; positive regulation of cell cycle; positive regulation of cyclin-dependent protein kinase activity; positive regulation of protein amino acid phosphorylation; response to DNA damage stimulus; response to drug Disease: Myeloma, Multiple; Von Hippel-lindau Syndrome |
NCBI Summary: | The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis. [provided by RefSeq, Jul 2008] |
UniProt Code: | P24385 |
NCBI GenInfo Identifier: | 116152 |
NCBI Gene ID: | 595 |
NCBI Accession: | P24385.1 |
UniProt Secondary Accession: | P24385,Q6LEF0, |
UniProt Related Accession: | P24385 |
Molecular Weight: | 34kDa |
NCBI Full Name: | G1/S-specific cyclin-D1 |
NCBI Synonym Full Names: | cyclin D1 |
NCBI Official Symbol: | CCND1Â Â |
NCBI Official Synonym Symbols: | BCL1; PRAD1; U21B31; D11S287EÂ Â |
NCBI Protein Information: | G1/S-specific cyclin-D1 |
UniProt Protein Name: | G1/S-specific cyclin-D1 |
UniProt Synonym Protein Names: | B-cell lymphoma 1 protein; BCL-1; BCL-1 oncogene; PRAD1 oncogene |
Protein Family: | G1/S-specific cyclin |
UniProt Gene Name: | CCND1Â Â |