Anti-ATP5B Antibody (RACO0328)
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주문- SKU:
- RACO0328
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- WB
- Application:
- ELISA
- Application:
- IHC
- Conjugation:
- Unconjugated
Description
Product Name: | ATP5B Antibody |
Product SKU: | RACO0328 |
Size: | 50ul |
Host Species: | Homo sapiens (Human) |
Tested Applications: | ELISA, WB, IHC |
Recommended Dilutions: | WB:1:500-1:5000, IHC:1:50-1:200 |
Species Reactivity: | Human, Mouse, Rat |
Immunogen: | A synthesized peptide derived from human ATPB |
Form: | Liquid |
Storage Buffer: | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Purification Method: | Affinity-chromatography |
Clonality: | Monoclonal |
Isotype: | Rabbit IgG |
Conjugate: | Non-conjugated |
Background: | Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. |
Synonyms: | ATP synthase subunit beta, mitochondrial (EC 3.6.3.14), ATP5B, ATPMB ATPSB |
IHC image of RACO0328 diluted at 1:100 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. | |
IHC image of RACO0328 diluted at 1:100 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. | |
Western Blot. Positive WB detected in 293T whole cell lysate HT29 whole cell lysate HepG2 whole cell lysate Jurkat whole cell lysate 293 whole cell lysate Rat Heart tissue, Mouse Heart tissue. All lanes ATP5F1B antibody at 1:2000. Secondary. Goat polyclonal to rabbit IgG at 1:50000 dilution. Predicted band size: 57 kDa. Observed band size: 57 kDa. |
UniProt Protein Function: | ATP5B: Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. Belongs to the ATPase alpha/beta chains family. |
UniProt Protein Details: | Protein type:EC 3.6.3.14; Hydrolase; Mitochondrial; Energy Metabolism - oxidative phosphorylation Chromosomal Location of Human Ortholog: 12q13.13 Cellular Component: cell surface; membrane; mitochondrial matrix; mitochondrial membrane; mitochondrial proton-transporting ATP synthase complex; mitochondrion; nucleus; plasma membrane Molecular Function:ATPase activity; hydrogen ion transporting ATPase activity, rotational mechanism; MHC class I protein binding; protein binding; transmembrane transporter activity Biological Process: angiogenesis; ATP biosynthetic process; mitochondrial ATP synthesis coupled proton transport; mitochondrion organization and biogenesis; osteoblast differentiation; proton transport; regulation of intracellular pH |
NCBI Summary: | This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the beta subunit of the catalytic core. [provided by RefSeq, Jul 2008] |
UniProt Code: | P06576 |
NCBI GenInfo Identifier: | 114549 |
NCBI Gene ID: | 506 |
NCBI Accession: | P06576.3 |
UniProt Secondary Accession: | P06576,Q14283, A8K4X0, |
UniProt Related Accession: | P06576 |
Molecular Weight: | 56,560 Da |
NCBI Full Name: | ATP synthase subunit beta, mitochondrial |
NCBI Synonym Full Names: | ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide |
NCBI Official Symbol: | ATP5BÂ Â |
NCBI Official Synonym Symbols: | ATPMB; ATPSB; HEL-S-271Â Â |
NCBI Protein Information: | ATP synthase subunit beta, mitochondrial |
UniProt Protein Name: | ATP synthase subunit beta, mitochondrial |
UniProt Gene Name: | ATP5BÂ Â |
UniProt Entry Name: | ATPB_HUMAN |