Anti-ATG5 항체 (CAB19677)

Antibody Name:	Anti-ATG5 Antibody [KO Validated]
Antibody SKU:	CAB19677
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthesized peptide derived from human ATG5.

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, SH-SY5Y, Mouse brain, Mouse lung, 293T

Immunogen:	A synthesized peptide derived from human ATG5.
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	9474
Uniprot:	Q9H1Y0
Cellular Location:
Calculated MW:	55kDa
Observed MW:	55KDa

	Western blot analysis of extracts from normal (control) and ATG5 knockout (KO) 293T cells, using ATG5 antibody (CAB19677) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Western blot analysis of extracts of various cell lines, using ATG5 antibody (CAB19677) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunohistochemistry of paraffin-embedded human placenta using ATG5 antibody (CAB19677) at dilution of 1:100 (40x lens).

Synonyms:

ATG5, APG5, APG5-LIKE, APG5L, ASP, hAPG5

Background:

The protein encoded by this gene, in combination with autophagy protein 12, functions as an E1-like activating enzyme in a ubiquitin-like conjugating system. The encoded protein is involved in several cellular processes, including autophagic vesicle formation, mitochondrial quality control after oxidative damage, negative regulation of the innate antiviral immune response, lymphocyte development and proliferation, MHC II antigen presentation, adipocyte differentiation, and apoptosis. Several transcript variants encoding different protein isoforms have been found for this gene. [provided by RefSeq, Sep 2015]

UniProt Code:	Q9H1Y0
NCBI GenInfo Identifier:	17366828
NCBI Gene ID:	9474
NCBI Accession:	Q9H1Y0.2
UniProt Secondary Accession:	Q9H1Y0,O60875, Q5JVR2, Q68DI4, Q9H2B8, Q9HCZ7,
UniProt Related Accession:	Q9H1Y0
Molecular Weight:	275
NCBI Full Name:	Autophagy protein 5
NCBI Synonym Full Names:	autophagy related 5
NCBI Official Symbol:	ATG5
NCBI Official Synonym Symbols:	ASP; APG5; APG5L; hAPG5; APG5-LIKE
NCBI Protein Information:	autophagy protein 5; apoptosis-specific protein; ATG5 autophagy related 5 homolog
UniProt Protein Name:	Autophagy protein 5
UniProt Synonym Protein Names:	APG5-like; Apoptosis-specific protein
Protein Family:	Autophagy protein
UniProt Gene Name:	ATG5
UniProt Entry Name:	ATG5_HUMAN

Antibodies	ELISA Kits
Anti-ATG5 Antibody (CAB0203)	Human ATG5 / Autophagy protein 5 ELISA Kit
Anti-ATG5 Antibody (CAB11427)	Human Autophagy protein 5 (ATG5) ELISA Kit
ATG5 Antibody (PACO07470)
ATG5 Antibody (PACO07906)

Secondary Antibody

Anti-HRP Goat Anti-Mouse IgG (H+L) Antibody (CABS003)