Anti-ATG4C Antibody (CAB7396)
- SKU:
- CAB7396
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Autophagy
Frequently bought together:
Description
| Antibody Name: | Anti-ATG4C Antibody |
| Antibody SKU: | CAB7396 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human ATG4C (NP_116241.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human |
| Positive Samples: | Jurkat, HL-60 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human ATG4C (NP_116241.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MEAT GTDE VDKL KTKF ISAW NNMK YSWV LKTK TYFS RNSP VLLL GKCY HFKY EDED KTLP AESG CTIE DHVI AGNV EEFR KDFI SRIW LTYR EEFP QIEG SALT TDCG WGCT LRTG QMLL AQGL ILHF LGRA WTWP DALN IENS DSES WTSH TVKK FTAS FEAS LSGE REFK TPTI SLKE TIGK YSDD HEMR NEVY HRKI |
| Gene ID: | 84938 |
| Uniprot: | Q96DT6 |
| Cellular Location: | Cytoplasm |
| Calculated MW: | 52kDa |
| Observed MW: | 52kDa |
| Synonyms: | ATG4C, APG4-C, APG4C, AUTL1, AUTL3 |
| Background: | Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Alternate transcriptional splice variants, encoding the same protein, have been characterized. |
| UniProt Protein Function: | ATG4C: Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. Highly expressed in skeletal muscle, heart, liver and testis. Inhibited by N-ethylmaleimide. Belongs to the peptidase C54 family. |
| UniProt Protein Details: | Protein type:Autophagy; Protease; Ubiquitin conjugating system; EC 3.4.22.- Chromosomal Location of Human Ortholog: 1p31.3 Cellular Component: cytoplasm; cytosol; extracellular region Molecular Function:cysteine-type endopeptidase activity; peptidase activity Biological Process: autophagic vacuole formation; autophagy; C-terminal protein lipidation; mitochondrion degradation; protein delipidation; protein processing; protein targeting to membrane; proteolysis |
| NCBI Summary: | Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Alternate transcriptional splice variants, encoding the same protein, have been characterized. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q96DT6 |
| NCBI GenInfo Identifier: | 61211867 |
| NCBI Gene ID: | 84938 |
| NCBI Accession: | Q96DT6.1 |
| UniProt Secondary Accession: | Q96DT6,Q96K04, A6NLR8, D3DQ58, |
| UniProt Related Accession: | Q96DT6 |
| Molecular Weight: | 52,497 Da |
| NCBI Full Name: | Cysteine protease ATG4C |
| NCBI Synonym Full Names: | autophagy related 4C cysteine peptidase |
| NCBI Official Symbol: | ATG4C |
| NCBI Official Synonym Symbols: | APG4C; AUTL1; AUTL3; APG4-C |
| NCBI Protein Information: | cysteine protease ATG4C |
| UniProt Protein Name: | Cysteine protease ATG4C |
| UniProt Synonym Protein Names: | AUT-like 3 cysteine endopeptidase; Autophagin-3; Autophagy-related cysteine endopeptidase 3; Autophagy-related protein 4 homolog C |
| Protein Family: | Cysteine protease |
| UniProt Gene Name: | ATG4C |
| UniProt Entry Name: | ATG4C_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ATG4C antibody (CAB7396) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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