Anti-ARNT Antibody (RACO0173)
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주문- SKU:
- RACO0173
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- IP
- Application:
- WB
- Application:
- ELISA
- Application:
- IHC
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Product Name: | ARNT Antibody |
Product SKU: | RACO0173 |
Size: | 50ul |
Host Species: | Human |
Tested Applications: | ELISA, WB, IHC, IP |
Recommended Dilutions: | WB:1:500-1:5000, IHC:1:50-1:200, IP:1:200-1:1000 |
Species Reactivity: | Human |
Immunogen: | A synthesized peptide derived from human ARNT |
Form: | Liquid |
Storage Buffer: | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Purification Method: | Affinity-chromatography |
Clonality: | Monoclonal |
Isotype: | Rabbit IgG |
Conjugate: | Non-conjugated |
Background: | Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The complex then initiates transcription of genes involved in the activation of PAH procarcinogens. The heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters and functions as a transcriptional regulator of the adaptive response to hypoxia (By similarity). The heterodimer ARNT:AHR binds to core DNA sequence 5'-TGCGTG-3' within the dioxin response element (DRE) of target gene promoters and activates their transcription (PubMed:28396409). |
Synonyms: | Aryl hydrocarbon receptor nuclear translocator, ARNT protein, Class E basic helix-loop-helix protein 2, bHLHe2, Dioxin receptor, nuclear translocator, Hypoxia-inducible factor 1-beta, HIF-1-beta, HIF1-beta, ARNT, BHLHE2 |
IHC image of RACO0173 diluted at 1:183 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. | |
Immunoprecipitating HIF-1 beta in Hela whole cell lysate Lane 1: Rabbit control IgG instead of RACO0173 in Hela whole cell lysate For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: RACO0173 (3µg) + Hela whole cell lysate (500µg). Lane 3: Hela whole cell lysate (20µg). | |
Western Blot. Positive WB detected in Hela whole cell lysate MCF-7 whole cell lysate HepG2 whole cell lysate K562 whole cell lysate All lanes ARNT antibody at 1. (3µg) ml. Secondary. Goat polyclonal to rabbit IgG at 1:50000 dilution. Predicted band size: 87, 85, 86 KDa. Observed band size: 87 KDa. |
UniProt Protein Function: | Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The complex then initiates transcription of genes involved in the activation of PAH procarcinogens. The heterodimer with HIF1A or EPAS1/HIF2A functions as a transcriptional regulator of the adaptive response to hypoxia. |
NCBI Summary: | This gene encodes a protein containing a basic helix-loop-helix domain and two characteristic PAS domains along with a PAC domain. The encoded protein binds to ligand-bound aryl hydrocarbon receptor and aids in the movement of this complex to the nucleus, where it promotes the expression of genes involved in xenobiotic metabolism. This protein is also a co-factor for transcriptional regulation by hypoxia-inducible factor 1. Chromosomal translocation of this locus with the ETV6 (ets variant 6) gene on chromosome 12 have been described in leukemias. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2013] |
UniProt Code: | P27540 |
NCBI GenInfo Identifier: | 114163 |
NCBI Gene ID: | 405 |
NCBI Accession: | P27540.1 |
UniProt Secondary Accession: | P27540,Q59ED4, Q5QP39, Q8NDC7, B2R9H1, C4AMA1, F8WAP6 |
UniProt Related Accession: | P27540 |
Molecular Weight: | 86,366 Da |
NCBI Full Name: | Aryl hydrocarbon receptor nuclear translocator |
NCBI Synonym Full Names: | aryl hydrocarbon receptor nuclear translocator |
NCBI Official Symbol: | ARNTÂ Â |
NCBI Official Synonym Symbols: | HIF1B; TANGO; bHLHe2; HIF1BETA; HIF-1beta; HIF1-beta; HIF-1-beta  |
NCBI Protein Information: | aryl hydrocarbon receptor nuclear translocator |
UniProt Protein Name: | Aryl hydrocarbon receptor nuclear translocator |
UniProt Synonym Protein Names: | Class E basic helix-loop-helix protein 2; bHLHe2; Dioxin receptor, nuclear translocator; Hypoxia-inducible factor 1-beta; HIF-1-beta; HIF1-beta |
Protein Family: | Aryl hydrocarbon receptor nuclear translocator |
UniProt Gene Name: | ARNTÂ Â |