Anti-ACTA1 Antibody (RACO0047)
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주문- SKU:
- RACO0047
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- WB
- Application:
- IF
- Application:
- ELISA
- Application:
- IHC
- Application:
- FC
- Conjugation:
- Unconjugated
Description
Product Name: | ACTA1 Antibody |
Product SKU: | RACO0047 |
Size: | 50ul |
Host Species: | Human |
Tested Applications: | ELISA, WB, IHC, IF, FC |
Recommended Dilutions: | WB:1:500-1:5000, IHC:1:50-1:500, IF:1:30-1:200 |
Species Reactivity: | Human |
Immunogen: | A synthesized peptide |
Form: | Liquid |
Storage Buffer: | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Purification Method: | Affinity-chromatography |
Clonality: | Monoclonal |
Isotype: | Rabbit IgG |
Conjugate: | Non-conjugated |
Background: | Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. |
Synonyms: | Actin, alpha skeletal muscle, Alpha-actin-1, ACTA1, ACTA |
IHC image of RACO0047 diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. | |
Immunofluorescence staining of HepG2 cells with RACO0047 at 1:60,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L). | |
Western Blot. Positive WB detected in Hela whole cell lysate A549 whole cell lysate Raw264.7 whole cell lysate NIH/3T3 whole cell lysate HepG2 whole cell lysate Rat brain tissue, Rat heart tissue. All lanes Actin antibody at 0.95µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1:50000 dilution. Predicted band size: 42 KDa. Observed band size: 42 KDa. |
UniProt Protein Function: | ACTA1: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Interacts with TTID. Interacts (via its C-terminus) with USP25; the interaction occurs for all USP25 isoforms but is strongest for isoform USP25m in muscle differentiating cells. Belongs to the actin family. |
UniProt Protein Details: | Protein type:Cell development/differentiation; Motility/polarity/chemotaxis; Contractile; Cytoskeletal Chromosomal Location of Human Ortholog: 1q42.13 Cellular Component: extracellular space; sarcomere; stress fiber; actin filament; cytosol; striated muscle thin filament; actin cytoskeleton Molecular Function:protein binding; myosin binding; structural constituent of cytoskeleton; ADP binding; ATP binding Biological Process: skeletal muscle fiber adaptation; muscle contraction; muscle thin filament assembly; response to steroid hormone stimulus; response to mechanical stimulus; response to lithium ion; response to extracellular stimulus; skeletal muscle fiber development; cell growth; muscle filament sliding Disease: Nemaline Myopathy 3; Myopathy, Congenital, With Fiber-type Disproportion |
NCBI Summary: | The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects. [provided by RefSeq, Jul 2008] |
UniProt Code: | P68133 |
NCBI GenInfo Identifier: | 4501881 |
NCBI Gene ID: | 58 |
NCBI Accession: | NP_001091.1 |
UniProt Secondary Accession: | P68133,P02568, P99020, Q5T8M9, |
UniProt Related Accession: | P68133 |
Molecular Weight: | 42,051 Da |
NCBI Full Name: | actin, alpha skeletal muscle |
NCBI Synonym Full Names: | actin, alpha 1, skeletal muscle |
NCBI Official Symbol: | ACTA1Â Â |
NCBI Official Synonym Symbols: | ACTA; ASMA; CFTD; MPFD; NEM1; NEM2; NEM3; CFTD1; CFTDMÂ Â |
NCBI Protein Information: | actin, alpha skeletal muscle; nemaline myopathy type 3 |
UniProt Protein Name: | Actin, alpha skeletal muscle |
UniProt Synonym Protein Names: | Alpha-actin-1 |
UniProt Gene Name: | ACTA1Â Â |
UniProt Entry Name: | ACTS_HUMAN |