ANO2 Antibody (PACO57548)
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주문- SKU:
- PACO57548
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Antibody Name: | ANO2 Antibody (PACO57548) |
Antibody SKU: | PACO57548 |
Size: | 50ug |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB, IHC, IF |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:500-1:1000, IF:1:200-1:500 |
Species Reactivity: | Human, Rat |
Immunogen: | Recombinant Human Anoctamin-2 protein (1-83AA) |
Form: | Liquid |
Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Purification Method: | >95%, Protein G purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
IHC image of PACO57548 diluted at 1:600 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. | |
Western Blot. Positive WB detected in: Hela whole cell lysate, U87 whole cell lysate, U251 whole cell lysate, MCF-7 whole cell lysate, Rat heart tissue. All lanes: ANO2 antibody at 3.9µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 114 kDa. Observed band size: 114 kDa. | |
Immunofluorescence staining of HepG2 cells with PACO57548 at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
Background: | Calcium-activated chloride channel (CaCC) which may play a role in olfactory signal transduction. Odorant molecules bind to odor-sensing receptors (OSRs), leading to an increase in calcium entry that activates CaCC current which amplifies the depolarization of the OSR cells, ANO2 seems to be the underlying chloride channel involved in this process. May mediate light perception amplification in retina. |
Synonyms: | Anoctamin-2 (Transmembrane protein 16B), ANO2, C12orf3 TMEM16B |
UniProt Protein Function: | ANO2: Acts as a calcium-activated chloride channel (CaCC), mostly in photoreceptors. May mediate olfactory amplification in olfactory sensory neurons (OSNs) and light perception amplification in retina. Belongs to the anoctamin family. 3 isoforms of the human protein are produced by alternative splicing. |
UniProt Protein Details: | Protein type:Transporter, ion channel; Transporter; Membrane protein, integral; Membrane protein, multi-pass Chromosomal Location of Human Ortholog: 12p13.3 Cellular Component: nucleoplasm; nonmotile primary cilium; plasma membrane Molecular Function:protein homodimerization activity; intracellular calcium activated chloride channel activity; protein heterodimerization activity Biological Process: transmembrane transport |
NCBI Summary: | ANO2 belongs to a family of calcium-activated chloride channels (CaCCs) (reviewed by Hartzell et al., 2009 [PubMed 19015192]).[supplied by OMIM, Jan 2011] |
UniProt Code: | Q9NQ90 |
NCBI GenInfo Identifier: | 262527528 |
NCBI Gene ID: | 57101 |
NCBI Accession: | Q9NQ90.2 |
UniProt Secondary Accession: | Q9NQ90,Q9H847, C4N787, |
UniProt Related Accession: | Q9NQ90 |
Molecular Weight: | 1003 |
NCBI Full Name: | Anoctamin-2 |
NCBI Synonym Full Names: | anoctamin 2, calcium activated chloride channel |
NCBI Official Symbol: | ANO2 |
NCBI Official Synonym Symbols: | C12orf3; TMEM16B |
NCBI Protein Information: | anoctamin-2; transmembrane protein 16B (eight membrane-spanning domains) |
UniProt Protein Name: | Anoctamin-2 |
UniProt Synonym Protein Names: | Transmembrane protein 16B |
Protein Family: | Anoctamin |
UniProt Gene Name: | ANO2 |
UniProt Entry Name: | ANO2_HUMAN |