ALK Antibody (PACO23031)
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주문- SKU:
- PACO23031
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Description
Antibody Name: | ALK Antibody (PACO23031) |
Antibody SKU: | PACO23031 |
Size: | 100ul |
Host Species: | Rabbit |
Tested Applications: | ELISA, IHC, IF |
Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:50-1:100, IF:1:100-1:500 |
Species Reactivity: | Human |
Immunogen: | Synthesized peptide derived from Internal of human ALK. |
Form: | Liquid |
Storage Buffer: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Purification Method: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
Immunohistochemistry analysis of paraffin-embedded human brain tissue using ALK antibody. | |
Immunofluorescence analysis of HeLa cells, using ALK antibody. |
Background: | Neuronal orphan receptor tyrosine kinase that is essentially and transiently expressed in specific regions of the central and peripheral nervous systems and plays an important role in the genesis and differentiation of the nervous system. Transduces signals from ligands at the cell surface, through specific activation of the mitogen-activated protein kinase (MAPK) pathway. Phosphorylates almost exclusively at the first tyrosine of the Y-x-x-x-Y-Y motif. Following activation by ligand, ALK induces tyrosine phosphorylation of CBL, FRS2, IRS1 and SHC1, as well as of the MAP kinases MAPK1/ERK2 and MAPK3/ERK1. Acts as a receptor for ligands pleiotrophin (PTN), a secreted growth factor, and midkine (MDK), a PTN-related factor, thus participating in PTN and MDK signal transduction. PTN-binding induces MAPK pathway activation, which is important for the anti-apoptotic signaling of PTN and regulation of cell proliferation. MDK-binding induces phosphorylation of the ALK target insulin receptor substrate (IRS1), activates mitogen-activated protein kinases (MAPKs) and PI3-kinase, resulting also in cell proliferation induction. Drives NF-kappa-B activation, probably through IRS1 and the activation of the AKT serine/threonine kinase. Recruitment of IRS1 to activated ALK and the activation of NF-kappa-B are essential for the autocrine growth and survival signaling of MDK. |
Synonyms: | ALK tyrosine kinase receptor precursor; Anaplastic lymphoma kinase; CD246; EC 2.7.10.1; kinase ALK |
UniProt Protein Function: | ALK: a tyrosine kinase of the ALK family. Plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system. Translocated and expressed as a fusion protein in anaplastic lymphoma. About one third of large-cell lymphomas are caused by a t(2;5)(p23;q35) translocation that fuses ALK to nucleophosmin (NPM1A). Other cases caused by fusions of ALK to moesin, non-muscle myosin heavy chain 9, clathrin heavy chain and other genes. Several fusions also seen in inflammatory myofibroblastic tumors, and expression has been briefly noted in a range of tumors |
UniProt Protein Details: | Protein type:EC 2.7.10.1; Protein kinase, TK; Membrane protein, integral; Kinase, protein; Protein kinase, tyrosine (receptor); Oncoprotein; TK group; Alk family Chromosomal Location of Human Ortholog: 2p23 Cellular Component: protein complex; integral to plasma membrane Molecular Function:NF-kappaB-inducing kinase activity; protein binding; protein-tyrosine kinase activity; transmembrane receptor protein tyrosine kinase activity; ATP binding Biological Process: regulation of apoptosis; cell proliferation; peptidyl-tyrosine phosphorylation; activation of MAPK activity; protein amino acid autophosphorylation; neuron development; signal transduction; phosphorylation; transmembrane receptor protein tyrosine kinase signaling pathway; activation of NF-kappaB transcription factor Disease: Neuroblastoma, Susceptibility To, 3 |
NCBI Summary: | This gene encodes a receptor tyrosine kinase, which belongs to the insulin receptor superfamily. This protein comprises an extracellular domain, an hydrophobic stretch corresponding to a single pass transmembrane region, and an intracellular kinase domain. It plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system. This gene has been found to be rearranged, mutated, or amplified in a series of tumours including anaplastic large cell lymphomas, neuroblastoma, and non-small cell lung cancer. The chromosomal rearrangements are the most common genetic alterations in this gene, which result in creation of multiple fusion genes in tumourigenesis, including ALK (chromosome 2)/EML4 (chromosome 2), ALK/RANBP2 (chromosome 2), ALK/ATIC (chromosome 2), ALK/TFG (chromosome 3), ALK/NPM1 (chromosome 5), ALK/SQSTM1 (chromosome 5), ALK/KIF5B (chromosome 10), ALK/CLTC (chromosome 17), ALK/TPM4 (chromosome 19), and ALK/MSN (chromosome X).[provided by RefSeq, Jan 2011] |
UniProt Code: | Q9UM73 |
NCBI GenInfo Identifier: | 296439447 |
NCBI Gene ID: | 238 |
NCBI Accession: | Q9UM73.3 |
UniProt Secondary Accession: | Q9UM73,Q4ZFX9, Q53QQ6, Q53RZ4, Q59FI3, Q9Y4K6, |
UniProt Related Accession: | Q9UM73 |
Molecular Weight: | 176,442 Da |
NCBI Full Name: | ALK tyrosine kinase receptor |
NCBI Synonym Full Names: | anaplastic lymphoma receptor tyrosine kinase |
NCBI Official Symbol: | ALK |
NCBI Official Synonym Symbols: | CD246; NBLST3 |
NCBI Protein Information: | ALK tyrosine kinase receptor; CD246 antigen; mutant anaplastic lymphoma kinase |
UniProt Protein Name: | ALK tyrosine kinase receptor |
UniProt Synonym Protein Names: | Anaplastic lymphoma kinase |
Protein Family: | ALK tyrosine kinase receptor |
UniProt Gene Name: | ALK |
UniProt Entry Name: | ALK_HUMAN |
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