Anti-UBE2R2 항체 (CAB7373)

Antibody Name:	Anti-UBE2R2 Antibody
Antibody SKU:	CAB7373
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-238 of human UBE2R2 (NP_060281.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Jurkat, MCF7, Mouse liver, Mouse kidney, Mouse heart, Rat brain, Rat lung

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-238 of human UBE2R2 (NP_060281.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAQQ QMTS SQKA LMLE LKSL QEEP VEGF RITL VDES DLYN WEVA IFGP PNTL YEGG YFKA HIKF PIDY PYSP PTFR FLTK MWHP NIYE NGDV CISI LHPP VDDP QSGE LPSE RWNP TQNV RTIL LSVI SLLN EPNT FSPA NVDA SVMF RKWR DSKG KDKE YAEI IRKQ VSAT KAEA EKDG VKVP TTLA EYCI KTKV PSND NSSD LLYD DLYD DDID DEDE EEED ADCY DDDD SGNE ES
Gene ID:	54926
Uniprot:	Q712K3
Cellular Location:
Calculated MW:	27kDa
Observed MW:	27kDa

Synonyms:

UBE2R2, CDC34B, E2-CDC34B, UBC3B

Background:

Protein kinase CK2 is a ubiquitous and pleiotropic Ser/Thr protein kinase involved in cell growth and transformation. This gene encodes a protein similar to the E2 ubiquitin conjugating enzyme UBC3/CDC34. Studies suggest that CK2-dependent phosphorylation of this ubiquitin-conjugating enzyme functions by regulating beta-TrCP substrate recognition and induces its interaction with beta-TrCP, enhancing beta-catenin degradation.

UniProt Protein Function:	UBC3B: an E2 ubiquitin conjugating enzyme similar to UBC3/CDC34. Phosphorylation of UBC3B by CK2 induces its interaction with beta-TrCP, regulating beta-TrCP substrate recognition and enhancing beta-catenin degradation.
UniProt Protein Details:	Protein type:EC 6.3.2.19; Ubiquitin ligase; Ligase; Ubiquitin conjugating system Chromosomal Location of Human Ortholog: 9p13.3 Cellular Component: cytoplasm Molecular Function:small conjugating protein ligase activity; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; ATP binding; ligase activity Biological Process: protein monoubiquitination; proteasomal ubiquitin-dependent protein catabolic process
NCBI Summary:	Protein kinase CK2 is a ubiquitous and pleiotropic Ser/Thr protein kinase involved in cell growth and transformation. This gene encodes a protein similar to the E2 ubiquitin conjugating enzyme UBC3/CDC34. Studies suggest that CK2-dependent phosphorylation of this ubiquitin-conjugating enzyme functions by regulating beta-TrCP substrate recognition and induces its interaction with beta-TrCP, enhancing beta-catenin degradation. [provided by RefSeq, Jul 2008]
UniProt Code:	Q712K3
NCBI GenInfo Identifier:	74749761
NCBI Gene ID:	54926
NCBI Accession:	Q712K3.1
UniProt Secondary Accession:	Q712K3,Q9NX64, D3DRL5,
UniProt Related Accession:	Q712K3
Molecular Weight:	238
NCBI Full Name:	Ubiquitin-conjugating enzyme E2 R2
NCBI Synonym Full Names:	ubiquitin-conjugating enzyme E2R 2
NCBI Official Symbol:	UBE2R2
NCBI Official Synonym Symbols:	UBC3B; CDC34B; E2-CDC34B
NCBI Protein Information:	ubiquitin-conjugating enzyme E2 R2; ubiquitin-protein ligase R2; ubiquitin carrier protein R2; ubiquitin-conjugating enzyme UBC3B; ubiquitin-conjugating enzyme E2-CDC34B
UniProt Protein Name:	Ubiquitin-conjugating enzyme E2 R2
UniProt Synonym Protein Names:	Ubiquitin carrier protein R2; Ubiquitin-conjugating enzyme E2-CDC34B; Ubiquitin-protein ligase R2
Protein Family:	Ubiquitin-conjugating enzyme
UniProt Gene Name:	UBE2R2
UniProt Entry Name:	UB2R2_HUMAN

	Western blot analysis of extracts of various cell lines, using UBE2R2 antibody (CAB7373) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded mouse cancer using UBE2R2 antibody (CAB7373) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of A549 cells using UBE2R2 antibody (CAB7373).

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)