Anti-TRPC5 항체 (CAB10089)

Antibody Name:	Anti-TRPC5 Antibody
Antibody SKU:	CAB10089
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 764-973 of human TRPC5 (NP_036603.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Rat
Positive Samples:	SH-SY5Y

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 764-973 of human TRPC5 (NP_036603.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	RKHP RSFS TSST ELSQ RDDN NDGS GGAR AKSK SVSF NLGC KKKT CHGP PLIR TMPR SSGA QGKS KAES SSKR SFMG PSLK KLGL LFSK FNGH MSEP SSEP MYTI SDGI VQQH CMWQ DIRY SQME KGKA EACS QSEI NLSE VELG EVQG AAQS SECP LACS SSLH CASS ICSS NSKL LDSS EDVF ETWG EACD LLMH KWGD GQEE QVTT RL
Gene ID:	7224
Uniprot:	Q9UL62
Cellular Location:	Cell membrane, Multi-pass membrane protein
Calculated MW:	111kDa
Observed MW:	111kDa

Synonyms:

TRPC5, PPP1R159, TRP5

Background:

This gene belongs to the transient receptor family. It encodes one of the seven mammalian TRPC (transient receptor potential channel) proteins. The encoded protein is a multi-pass membrane protein and is thought to form a receptor-activated non-selective calcium permeant cation channel. The protein is active alone or as a heteromultimeric assembly with TRPC1, TRPC3, and TRPC4. It also interacts with multiple proteins including calmodulin, CABP1, enkurin, Na(+)-H+ exchange regulatory factor (NHERF ), interferon-induced GTP-binding protein (MX1), ring finger protein 24 (RNF24), and SEC14 domain and spectrin repeat-containing protein 1 (SESTD1).

UniProt Protein Function:	TRPC5: Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Has also been shown to be calcium-selective. May also be activated by intracellular calcium store depletion. Belongs to the transient receptor (TC 1.A.4) family. STrpC subfamily. TRPC5 sub-subfamily.
UniProt Protein Details:	Protein type:Channel, cation; Membrane protein, integral; Membrane protein, multi-pass Chromosomal Location of Human Ortholog: Xq23 Cellular Component: integral to plasma membrane; plasma membrane Molecular Function:calcium channel activity; protein binding; store-operated calcium channel activity Biological Process: calcium ion transport; cytosolic calcium ion homeostasis; manganese ion transport; nervous system development
NCBI Summary:	This gene belongs to the transient receptor family. It encodes one of the seven mammalian TRPC (transient receptor potential channel) proteins. The encoded protein is a multi-pass membrane protein and is thought to form a receptor-activated non-selective calcium permeant cation channel. The protein is active alone or as a heteromultimeric assembly with TRPC1, TRPC3, and TRPC4. It also interacts with multiple proteins including calmodulin, CABP1, enkurin, Na(+)-H+ exchange regulatory factor (NHERF ), interferon-induced GTP-binding protein (MX1), ring finger protein 24 (RNF24), and SEC14 domain and spectrin repeat-containing protein 1 (SESTD1). [provided by RefSeq, May 2010]
UniProt Code:	Q9UL62
NCBI GenInfo Identifier:	10720321
NCBI Gene ID:	7224
NCBI Accession:	Q9UL62.1
UniProt Secondary Accession:	Q9UL62,O75233, Q5JXY8, Q9Y514, B2RP53,
UniProt Related Accession:	Q9UL62
Molecular Weight:	111,412 Da
NCBI Full Name:	Short transient receptor potential channel 5
NCBI Synonym Full Names:	transient receptor potential cation channel subfamily C member 5
NCBI Official Symbol:	TRPC5
NCBI Official Synonym Symbols:	TRP5; PPP1R159
NCBI Protein Information:	short transient receptor potential channel 5
UniProt Protein Name:	Short transient receptor potential channel 5
UniProt Synonym Protein Names:	Transient receptor protein 5; TRP-5; hTRP-5; hTRP5
Protein Family:	Short transient receptor potential channel
UniProt Gene Name:	TRPC5
UniProt Entry Name:	TRPC5_HUMAN

Western blot analysis of extracts of SH-SY5Y cells, using TRPC5 antibody (CAB10089) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)