Anti-TRPC3 항체 (CAB7742)

Antibody Name:	Anti-TRPC3 Antibody
Antibody SKU:	CAB7742
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide of human TRPC3

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	BT-474, HeLa, 22Rv1, 293T, Mouse brain, Rat spinal cord

Immunogen:	A synthetic peptide of human TRPC3
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	7222
Uniprot:	Q13507
Cellular Location:	Membrane, Multi-pass membrane protein
Calculated MW:	96kDa/97kDa/105kDa
Observed MW:	96kDa

Synonyms:

TRPC3, SCA41, TRP3

Background:

The protein encoded by this gene is a membrane protein that can form a non-selective channel permeable to calcium and other cations. The encoded protein appears to be induced to form channels by a receptor tyrosine kinase-activated phosphatidylinositol second messenger system and also by depletion of intracellular calcium stores. Two transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	TRPC3 iso3: Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Activated by diacylglycerol (DAG) in a membrane-delimited fashion, independently of protein kinase C, and by inositol 1,4,5- triphosphate receptors (ITPR) with bound IP3. May also be activated by internal calcium store depletion. Belongs to the transient receptor (TC 1.A.4) family. STrpC subfamily. TRPC3 sub-subfamily. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Membrane protein, integral; Membrane protein, multi-pass; Channel, cation Chromosomal Location of Human Ortholog: 4q27 Cellular Component: integral to plasma membrane; plasma membrane Molecular Function:protein binding; store-operated calcium channel activity; calcium channel activity Biological Process: axon guidance; platelet activation; calcium ion transport; phototransduction; response to calcium ion; blood coagulation; transmembrane transport; response to ATP Disease: Spinocerebellar Ataxia 41
NCBI Summary:	The protein encoded by this gene is a membrane protein that can form a non-selective channel permeable to calcium and other cations. The encoded protein appears to be induced to form channels by a receptor tyrosine kinase-activated phosphatidylinositol second messenger system and also by depletion of intracellular calcium stores. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
UniProt Code:	Q13507
NCBI GenInfo Identifier:	4507687
NCBI Gene ID:	7222
NCBI Accession:	NP_003296.1
UniProt Secondary Accession:	Q13507,O00593, Q15660, Q52M35, Q5G1L5, A7VJS1, E9PCJ9
UniProt Related Accession:	Q13507
Molecular Weight:	97,355 Da
NCBI Full Name:	short transient receptor potential channel 3 isoform b
NCBI Synonym Full Names:	transient receptor potential cation channel, subfamily C, member 3
NCBI Official Symbol:	TRPC3
NCBI Official Synonym Symbols:	TRP3
NCBI Protein Information:	short transient receptor potential channel 3; hTrp-3; transient receptor protein 3; transient receptor potential cation channel subfamily C member 3 variant c
UniProt Protein Name:	Short transient receptor potential channel 3
UniProt Synonym Protein Names:	Transient receptor protein 3; TRP-3; hTrp-3; hTrp3
Protein Family:	Short transient receptor potential channel
UniProt Gene Name:	TRPC3
UniProt Entry Name:	TRPC3_HUMAN

	Western blot analysis of extracts of various cell lines, using TRPC3 antibody (CAB7742) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded mouse brain using TRPC3 antibody (CAB7742) (40x lens).
	Immunohistochemistry of paraffin-embedded rat brain using TRPC3 antibody (CAB7742) (40x lens).
	Immunohistochemistry of paraffin-embedded human stomach using TRPC3 antibody (CAB7742) (40x lens).

Antibodies

TRPC3 Antibody (PACO02188)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)