Anti-STAMBP 항체 (CAB7065)

Antibody Name:	Anti-STAMBP Antibody
Antibody SKU:	CAB7065
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF IP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 100-270 of human STAMBP (NP_998787.1).

Application:	WB IHC IF IP
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100 IP 1:20 - 1:50
Reactivity:	Human, Mouse, Rat
Positive Samples:	MCF7, SW480, HepG2, HeLa, 293T, Mouse brain, Rat lung, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 100-270 of human STAMBP (NP_998787.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	FPKA EELK AELL KRYT KEYT EYNE EKKK EAEE LARN MAIQ QELE KEKQ RVAQ QKQQ QLEQ EQFH AFEE MIRN QELE KERL KIVQ EFGK VDPG LGGP LVPD LEKP SLDV FPTL TVSS IQPS DCHT TVRP AKPP VVDR SLKP GALS NSES IPTI DGLR HVVV PGRL CPQF LQL
Gene ID:	10617
Uniprot:	O95630
Cellular Location:	Cytoplasm, Early endosome, Membrane, Nucleus, Peripheral membrane protein
Calculated MW:	39kDa/48kDa
Observed MW:	48kDa

Synonyms:

STAMBP, AMSH, MICCAP

Background:

Cytokine-mediated signal transduction in the JAK-STAT cascade requires the involvement of adaptor molecules. One such signal-transducing adaptor molecule contains an SH3 domain that is required for induction of MYC and cell growth. The protein encoded by this gene binds to the SH3 domain of the signal-transducing adaptor molecule, and plays a critical role in cytokine-mediated signaling for MYC induction and cell cycle progression. Multiple alternatively spliced transcript variants encoding the same protein isoform have been found for this gene.

UniProt Protein Function:	AMSH: an adaptor protein involved in cytokine signal transduction the JAK-STAT cascade. Binds to the SH3 domain of the signal-transducing adaptor molecule STAM (signal transducing adaptor molecule). May play a critical role in cytokine-mediated signaling for MYC induction and cell cycle progression.
UniProt Protein Details:	Protein type:EC 3.1.2.15; Protease; Ubiquitin-specific protease; EC 3.4.19.- Chromosomal Location of Human Ortholog: 2p13.1 Cellular Component: cleavage furrow; cytoplasm; nucleoplasm; nucleus; plasma membrane Molecular Function:protein binding; protein domain specific binding; ubiquitin-specific protease activity Biological Process: cytokinesis after mitosis; JAK-STAT cascade; negative regulation of phosphoinositide 3-kinase cascade; negative regulation of Ras protein signal transduction; positive regulation of cell proliferation; protein deubiquitination Disease: Microcephaly-capillary Malformation Syndrome
NCBI Summary:	Cytokine-mediated signal transduction in the JAK-STAT cascade requires the involvement of adaptor molecules. One such signal-transducing adaptor molecule contains an SH3 domain that is required for induction of MYC and cell growth. The protein encoded by this gene binds to the SH3 domain of the signal-transducing adaptor molecule, and plays a critical role in cytokine-mediated signaling for MYC induction and cell cycle progression. Multiple alternatively spliced transcript variants encoding the same protein isoform have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:	O95630
NCBI GenInfo Identifier:	71153538
NCBI Gene ID:	10617
NCBI Accession:	O95630.1
UniProt Secondary Accession:	O95630,Q3MJE7, B5M0B6, D6W5H7,
UniProt Related Accession:	O95630
Molecular Weight:	39,596 Da
NCBI Full Name:	STAM-binding protein
NCBI Synonym Full Names:	STAM binding protein
NCBI Official Symbol:	STAMBP
NCBI Official Synonym Symbols:	AMSH; MICCAP
NCBI Protein Information:	STAM-binding protein
UniProt Protein Name:	STAM-binding protein
UniProt Synonym Protein Names:	Associated molecule with the SH3 domain of STAM; Endosome-associated ubiquitin isopeptidase
Protein Family:	STAM-binding protein
UniProt Gene Name:	STAMBP
UniProt Entry Name:	STABP_HUMAN

	Western blot analysis of extracts of various cell lines, using STAMBP antibody (CAB7065) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded rat spleen using STAMBP antibody (CAB7065) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human stomach using STAMBP antibody (CAB7065) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U2OS cells using STAMBP antibody (CAB7065). Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
STAMBP Antibody (PACO12503)	Human alpha-MSH ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)