Anti-SPINT1 Antibody (CAB6411)
- SKU:
- CAB6411
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-SPINT1 Antibody |
| Antibody SKU: | CAB6411 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 234-513 of human SPINT1 (NP_003701.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Mouse liver, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 234-513 of human SPINT1 (NP_003701.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | ANVT VTVL STKQ TEDY CLAS NKVG RCRG SFPR WYYD PTEQ ICKS FVYG GCLG NKNN YLRE EECI LACR GVQG PSME RRHP VCSG TCQP TQFR CSNG CCID SFLE CDDT PNCP DASD EAAC EKYT SGFD ELQR IHFP SDKG HCVD LPDT GLCK ESIP RWYY NPFS EHCA RFTY GGCY GNKN NFEE EQQC LESC RGIS KKDV FGLR REIP IPST GSVE MAVA VFLV ICIV VVVA ILGY CFFK NQRK DFHG HHHH PPPT PASS TVST TEDT EHLV YNHT TRPL |
| Gene ID: | 6692 |
| Uniprot: | O43278 |
| Cellular Location: | Secreted |
| Calculated MW: | 56kDa/58kDa |
| Observed MW: | 70kDa |
| Synonyms: | SPINT1, HAI, HAI1, MANSC2 |
| Background: | The protein encoded by this gene is a member of the Kunitz family of serine protease inhibitors. The protein is a potent inhibitor specific for HGF activator and is thought to be involved in the regulation of the proteolytic activation of HGF in injured tissues. Alternative splicing results in multiple variants encoding different isoforms. |
| UniProt Protein Function: | SPINT1: Inhibitor of HGF activator. Also acts as an inhibitor of matriptase (ST14). 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Secreted; Secreted, signal peptide Chromosomal Location of Human Ortholog: 15q15.1 Cellular Component: extracellular space; membrane; extracellular region Molecular Function:serine-type endopeptidase inhibitor activity Biological Process: extracellular matrix organization and biogenesis |
| NCBI Summary: | The protein encoded by this gene is a member of the Kunitz family of serine protease inhibitors. The protein is a potent inhibitor specific for HGF activator and is thought to be involved in the regulation of the proteolytic activation of HGF in injured tissues. Alternative splicing results in multiple variants encoding different isoforms. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O43278 |
| NCBI GenInfo Identifier: | 61252335 |
| NCBI Gene ID: | 6692 |
| NCBI Accession: | O43278.2 |
| UniProt Secondary Accession: | O43278,Q7Z7D2, |
| UniProt Related Accession: | O43278 |
| Molecular Weight: | 529 |
| NCBI Full Name: | Kunitz-type protease inhibitor 1 |
| NCBI Synonym Full Names: | serine peptidase inhibitor, Kunitz type 1 |
| NCBI Official Symbol: | SPINT1 |
| NCBI Official Synonym Symbols: | HAI; HAI1; MANSC2 |
| NCBI Protein Information: | kunitz-type protease inhibitor 1; HAI-1; serine protease inhibitor, Kunitz type 1; hepatocyte growth factor activator inhibitor type 1 |
| UniProt Protein Name: | Kunitz-type protease inhibitor 1 |
| UniProt Synonym Protein Names: | Hepatocyte growth factor activator inhibitor type 1; HAI-1 |
| Protein Family: | Kunitz-type protease inhibitor |
| UniProt Gene Name: | SPINT1 |
| UniProt Entry Name: | SPIT1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using SPINT1 antibody (CAB6411) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded human prostate using SPINT1 antibody (CAB6411) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse esophageal cancer using SPINT1 antibody (CAB6411) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of HeLa cells using SPINT1 antibody (CAB6411). Blue: DAPI for nuclear staining. |
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