Anti-SARDH Antibody (CAB9541)
- SKU:
- CAB9541
- Product type:
- Antibody
- Application:
- WB
- Reactivity:
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-SARDH Antibody |
| Antibody SKU: | CAB9541 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 300-560 of human SARDH (NP_009032.2). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:100 |
| Reactivity: | Mouse, Rat |
| Positive Samples: | Mouse brain, Mouse liver, Rat liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 300-560 of human SARDH (NP_009032.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | RIEG IQNM PNVR DHDA SVYL RLQG DALS VGGY EANP IFWE EVSD KFAF GLFD LDWE VFTQ HIEG AINR VPVL EKTG IKST VCGP ESFT PDHK PLMG EAPE LRGF FLGC GFNS AGMM LGGG CGQE LAHW IIHG RPEK DMHG YDIR RFHH SLTD HPRW IRER SHES YAKN YSVV FPHD EPLA GRNM RRDP LHEE LLGQ GCVF QERH GWER PGWF HPRG PAPV LEYD YYGA YGSR AHED YAYR RLLA DEYT FAFP PHHD TIKK E |
| Gene ID: | 1757 |
| Uniprot: | Q9UL12 |
| Cellular Location: | Mitochondrion matrix |
| Calculated MW: | 82kDa/101kDa |
| Observed MW: | 101kDa |
| Synonyms: | SARDH, BPR-2, DMGDHL1, SAR, SARD, SDH |
| Background: | This gene encodes an enzyme localized to the mitochondrial matrix which catalyzes the oxidative demethylation of sarcosine. This enzyme is distinct from another mitochondrial matrix enzyme, dimethylglycine dehydrogenase, which catalyzes a reaction resulting in the formation of sarcosine. Mutations in this gene are associated with sarcosinemia. Alternatively spliced transcript variants have been described. |
| UniProt Protein Function: | SARDH: Defects in SARDH are a cause of sarcosinemia (SAR). Sarcosinemia is a metabolic disorder characterized by an increased concentration of sarcosine in plasma and urine due to sarcosine dehydrogenase deficiency. Prevalence has been estimated at 1:28'000 to 1:350'000 in newborn screening programs. Sarcosinemia is most probably a benign condition without significant clinical problems. It is transmitted in an autosomal recessive manner. Belongs to the GcvT family. |
| UniProt Protein Details: | Protein type:Amino Acid Metabolism - glycine, serine and threonine; Oxidoreductase; EC 1.5.8.3; Mitochondrial Chromosomal Location of Human Ortholog: 9q33-q34 Cellular Component: mitochondrion; mitochondrial matrix Molecular Function:sarcosine dehydrogenase activity Disease: Sarcosinemia |
| NCBI Summary: | This gene encodes an enzyme localized to the mitochondrial matrix which catalyzes the oxidative demethylation of sarcosine. This enzyme is distinct from another mitochondrial matrix enzyme, dimethylglycine dehydrogenase, which catalyzes a reaction resulting in the formation of sarcosine. Mutations in this gene are associated with sarcosinemia. Alternatively spliced transcript variants have been described. [provided by RefSeq, Oct 2008] |
| UniProt Code: | Q9UL12 |
| NCBI GenInfo Identifier: | 21361378 |
| NCBI Gene ID: | 1757 |
| NCBI Accession: | NP_009032.2 |
| UniProt Related Accession: | Q9UL12 |
| Molecular Weight: | 105kDa |
| NCBI Full Name: | sarcosine dehydrogenase, mitochondrial |
| NCBI Synonym Full Names: | sarcosine dehydrogenase |
| NCBI Official Symbol: | SARDH |
| NCBI Official Synonym Symbols: | SAR; SDH; SARD; BPR-2; DMGDHL1 |
| NCBI Protein Information: | sarcosine dehydrogenase, mitochondrial |
| UniProt Protein Name: | Sarcosine dehydrogenase, mitochondrial |
| UniProt Synonym Protein Names: | BPR-2 |
| Protein Family: | Sarcosine dehydrogenase |
| UniProt Gene Name: | SARDH |
| UniProt Entry Name: | SARDH_HUMAN |
 | Western blot analysis of extracts of various cell lines, using SARDH Antibody (CAB9541) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunofluorescence analysis of L929 cells using Anti-SARDH Polyclonal Antibody (CAB9541) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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