Anti-PRMT3 Antibody (CAB13068)[KO Validated]

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SKU:
CAB13068
Product type:
Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
Frequently bought together:

Description

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Antibody Name:Anti-PRMT3 Antibody [KO Validated]
Antibody SKU:CAB13068
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human PRMT3 (NP_005779.1).
Application:WB
Recommended Dilution:WB 1:1000 - 1:2000
Reactivity:Human, Mouse
Positive Samples:Mouse brain, 293T
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human PRMT3 (NP_005779.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MCSL ASGA TGGR GAVE NEED LPEL SDSG DEAA WEDE DDAD LPHG KQQT PCLF CNRL FTSA EETF SHCK SEHQ FNID SMVH KHGL EFYG YIKL INFI RLKN PTVE YMNS IYNP VPWE KEEY LKPV LEDD LLLQ FDVE DLYE PVSV PFSY PNGL SENT SVVE KLKH MEAR ALSA EAAL ARAR EDLQ KMKQ FAQD FVMH TDVR TCSS STSV IADL QEDE DGVY
Gene ID:10196
Uniprot:O60678
Cellular Location:Cytoplasm
Calculated MW:52kDa/59kDa
Observed MW:60kDa
Synonyms:PRMT3, HRMT1L3
Background:This gene belongs to the protein arginine methyltransferase (PRMT) family. The encoded enzyme catalyzes the methylation of guanidino nitrogens of arginyl residues of proteins. The enzyme acts on 40S ribosomal protein S2 (rpS2), which is its major in-vivo substrate, and is involved in the proper maturation of the 80S ribosome. Alternative splicing results in multiple transcript variants.
UniProt Protein Function:PRMT3: Methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in some proteins. Belongs to the protein arginine N-methyltransferase family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:C2H2-type zinc finger protein; EC 2.1.1.-; Methyltransferase; Methyltransferase, protein arginine

Chromosomal Location of Human Ortholog: 11p15.1

Cellular Component: cytosol

Molecular Function:histone-arginine N-methyltransferase activity; methyltransferase activity; protein binding; protein-arginine omega-N asymmetric methyltransferase activity

Biological Process: negative regulation of protein ubiquitination; regulation of transcription, DNA-dependent

NCBI Summary:This gene belongs to the protein arginine methyltransferase (PRMT) family. The encoded enzyme catalyzes the methylation of guanidino nitrogens of arginyl residues of proteins. The enzyme acts on 40S ribosomal protein S2 (rpS2), which is its major in-vivo substrate, and is involved in the proper maturation of the 80S ribosome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]
UniProt Code:O60678
NCBI GenInfo Identifier:44771198
NCBI Gene ID:10196
NCBI Accession:NP_005779.1
UniProt Related Accession:O60678
Molecular Weight:52kDa; 59kDa
NCBI Full Name:protein arginine N-methyltransferase 3 isoform 1
NCBI Synonym Full Names:protein arginine methyltransferase 3
NCBI Official Symbol:PRMT3  
NCBI Official Synonym Symbols:HRMT1L3  
NCBI Protein Information:protein arginine N-methyltransferase 3
UniProt Protein Name:Protein arginine N-methyltransferase 3
UniProt Synonym Protein Names:Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 3
Protein Family:Protein arginine N-methyltransferase
UniProt Gene Name:PRMT3  
UniProt Entry Name:ANM3_HUMAN
Anti-PRMT3 Antibody [KO Validated] (CAB13068)Western blot analysis of extracts of mouse brain, using PRMT3 antibody (CAB13068) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-PRMT3 Antibody [KO Validated] (CAB13068)Western blot analysis of extracts from normal (control) and PRMT3 Rabbit pAb knockout (KO) 293T cells, using PRMT3 Rabbit pAb antibody (CAB13068) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.

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