Anti-NUCB1 Antibody (CAB3994)[KO Validated]

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SKU:
CAB3994
Product type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
Frequently bought together:

Description

system_update_alt데이터시트system_update_alt안전 데이터시트
Antibody Name:Anti-NUCB1 Antibody [KO Validated]
Antibody SKU:CAB3994
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 302-461 of human NUCB1 (NP_006175.2).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:A-549, HepG2, 293T, BxPC-3
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 302-461 of human NUCB1 (NP_006175.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:KNVD TNQD RLVT LEEF LAST QRKE FGDT GEGW ETVE MHPA YTEE ELRR FEEE LAAR EAEL NAKA QRLS QETE ALGR SQGR LEAQ KREL QQAV LHME QRKQ QQQQ QQGH KAPA AHPE GQLK FHPD TDDV PVPA PAGD QKEV DTSE KKLL ERLP EVEV PQHL
Gene ID:4924
Uniprot:Q02818
Cellular Location:Cytoplasm, Golgi apparatus, Lumenal side, Peripheral membrane protein, cis-Golgi network membrane
Calculated MW:53kDa
Observed MW:60kDa
Synonyms:NUCB1, CALNUC, NUC
Background:This gene encodes a member of a small calcium-binding EF-hand protein family. The encoded protein is thought to have a key role in Golgi calcium homeostasis and Ca(2+)-regulated signal transduction events.
UniProt Protein Function:NUCB1: Major calcium-binding protein of the Golgi. May have a role in calcium homeostasis. Belongs to the nucleobindin family.
UniProt Protein Details:

Protein type:DNA-binding

Chromosomal Location of Human Ortholog: 19q13.33

Cellular Component: microtubule cytoskeleton; Golgi apparatus; extracellular space; membrane; ER-Golgi intermediate compartment

Molecular Function:protein binding; DNA binding; calcium ion binding

NCBI Summary:This gene encodes a member of a small calcium-binding EF-hand protein family. The encoded protein is thought to have a key role in Golgi calcium homeostasis and Ca(2+)-regulated signal transduction events. [provided by RefSeq, Jun 2010]
UniProt Code:Q02818
NCBI GenInfo Identifier:90110780
NCBI Gene ID:4924
NCBI Accession:Q02818.4
UniProt Secondary Accession:Q02818,Q15838, Q7Z4J7, Q9BUR1, B2RD64,
UniProt Related Accession:Q02818
Molecular Weight:461
NCBI Full Name:Nucleobindin-1
NCBI Synonym Full Names:nucleobindin 1
NCBI Official Symbol:NUCB1  
NCBI Official Synonym Symbols:NUC; CALNUC  
NCBI Protein Information:nucleobindin-1
UniProt Protein Name:Nucleobindin-1
UniProt Synonym Protein Names:CALNUC
Protein Family:Nucleobindin
UniProt Gene Name:NUCB1  
UniProt Entry Name:NUCB1_HUMAN
Anti-NUCB1 Antibody [KO Validated] (CAB3994)Western blot analysis of extracts of various cell lines, using NUCB1 antibody (CAB3994) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-NUCB1 Antibody [KO Validated] (CAB3994)Western blot analysis of extracts from normal (control) and NUCB1 knockout (KO) 293T cells, using NUCB1 antibody (CAB3994) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s.
Anti-NUCB1 Antibody [KO Validated] (CAB3994)Immunofluorescence analysis of L929 cells using NUCB1 Polyclonal Antibody (CAB3994) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Anti-NUCB1 Antibody [KO Validated] (CAB3994)Immunofluorescence analysis of U-2 OS cells using NUCB1 Polyclonal Antibody (CAB3994) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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